journal article·2025-05-06Open access

Bacillus sp. GLN Laccase Characterization: Industry and Biotechnology Implications

Asemahle Gogotya, Nonso E. Nnolim, Uchechukwu U. Nwodo

Applied Sciences· Volume 15· Issue 9

Abstract

Laccases are multicopper oxidases that exhibit a broad substrate spectrum, making them excellent biocatalysts for clean technological processes. The study isolated novel laccase-producing bacteria from decomposed wood samples and characterized the enzyme for potential industrial and biotechnological applications. The results showed that three bacteria, SP-2, SP-1, and WP-2, out of the eight isolated bacteria, oxidized both guaiacol and α-naphthol in the plate assay and exhibited extracellular laccase activity of 7.0 ± 0.01, 6.67 ± 0.02, and 7.40 ± 0.04 (U/mL), respectively. WP-2 was selected for further study and was identified as Bacillus sp. GLN (accessioned number: MK290989). Strain GLN maximally secreted laccase 48 h post-fermentation, with an enzyme yield of 36.83 ± 2.47 U/mL in optimized conditions. The crude laccase was optimally active at pH 9.0 and 90 °C and showed excellent pH and thermal stability, retaining approximately 65% residual activity at 100 °C for 270 min. GLN laccase demonstrated remarkable stability after treatment with organic solvents and metal ions, retaining more than 50% of its original activity in the presence of 100 mM inhibitors. The data from this study highlight the relevance of Bacillus sp. GLN and its laccase in promoting clean technology.
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Research context

OpenAlex topics: Enzyme-mediated dye degradation · Microbial Metabolism and Applications · bioluminescence and chemiluminescence research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Patho-Biocatalysis Group (PBG), Department of Biotechnology and Biological Sciences, University of Fort Hare, Private Bag X1314, Alice 5700, Eastern Cape, South Africa

Funding

Department of Science and Innovation and the Technology Innovation Agency

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