Enrichment of Z-DNA-Forming Sequences Within Super-Enhancers: A Computational and Population-Based Study
Source abstract
Abstract
Super-enhancers (SEs) orchestrate high-level transcription by integrating multiple regulatory elements and signals. Although chromatin accessibility and transcription factor binding within SEs are extensively studied, the role of non-canonical DNA structures, particularly Z-DNA, remains underexplored. In this study, genome-wide predictions of Z-DNA-forming sequences (generated by the Z-DNA-BERT model) were applied to systematically investigate their distribution within typical enhancers and SEs across multiple human cancer cell lines. Statistically significant enrichment of Z-DNA sequences within SE regions, compared to random genomic controls, was observed. Furthermore, genetic variants overlapping these Z-DNA regions, identified using data from the 1000 Genomes Project, were found to alter binding motifs of the SP/KLF transcription factor family. These mutations exhibited population-specific clustering and overlapped previously reported pathogenic copy-number variations (CNVs) associated with neurodevelopmental disorders, potentially affecting transcription factor binding motifs related to neuronal growth and differentiation pathways. Population-level phylogenetic analysis revealed distinct clustering patterns of these variants, suggesting frequency-specific genetic architecture. Overall, the computational findings indicate that Z-DNA structures within super-enhancers might play regulatory roles and potentially influence population-specific genetic variation, highlighting specific genomic targets and providing new avenues for future experimental research.
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Research context
OpenAlex topics: Genomics and Chromatin Dynamics · DNA and Nucleic Acid Chemistry · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., 119991 Moscow, Russia
Faculty of Computer Science, Higher School of Economics, Myasnitskaya St. 20, 101000 Moscow, Russia
Institute for Information Transmission Problems RAS, 127051 Moscow, Russia
Funding
Russian Science Foundation
References (66)
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