journal article·2025-02-10Open access

Origin and Variety Identification of Dried Kelp Based on Fluorescence Fingerprinting and Machine Learning Approaches

Kana Suzuki, Rikuto Akiyama, Yvan Llave, Takashi Matsumoto

Applied Sciences· Volume 15· Issue 4

Abstract

Accurate labeling of the origin of food ingredients is essential to ensure quality and safety; however, establishing a reliable identification method remains an urgent task. The origin and variety of dried kelp are generally identified based on their morphological characteristics; however, they are difficult to distinguish unless experts are involved. In addition, genetically close varieties have almost no differences in their base sequences; therefore, the accuracy of conventional identification methods using genetic analysis is limited. This study aimed to develop a system for identifying the origin and variety of dried kelp using fluorescence fingerprint data obtained by fluorescence spectroscopy and a convolutional neural network (CNN). The fluorescence characteristics of dried kelp were measured in the range between 250 and 550 nm. The obtained fluorescence fingerprint data were converted into image data and analyzed using a CNN model implemented in Python, TensorFlow, and Keras. Unlike conventional methods that rely on morphological characteristics and genetic analyses, by combining fluorescence spectroscopy and CNN, a high identification accuracy of 98.86% was achieved even for genetically close varieties. These results highlight the excellent potential of fluorescent fingerprints in identifying the origin and variety of food and are believed to contribute to preventing food fraud and quality control.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Bee Products Chemical Analysis · Meat and Animal Product Quality · Essential Oils and Antimicrobial Activity

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan

Funding

JSPS KAKENHI

References (24)

Show source-provided references
1. Ministry of Agriculture, Forestry and Fisheries (2024, December 25). About the System for Labeling the Origin of Ingredients in Processed Foods. (In Japanese).
2. (2024, December 25). European Union Laws. Available online: https://eur-lex.europa.eu/legal-content/EN/ALL/?uri=CELEX:32018R0775.
3. (2024, December 25). Code of Federal Regulations, Available online: https://www.ecfr.gov/current/title-19/chapter-I/part-134.
4. Determination of the geographic origin of dried kelp by inorganic analysis10.2331/suisan.75.77 ↗
5. Determination of the geographic origin of boiled and salted wakame Undaria pinnatifida products by element analysis10.2331/suisan.77.243 ↗
6. Shimizu, K., Kato, Y., Kato, S., Inoue, A., Ojima, T., and Yasokawa, D. (2010). Technology for Geographic Origin Identification of Edible Kelps, Hokkaido Industrial Technology Center. (In Japanese).
7. Current remarks of phylogeny and taxonomy on genus Laminaria
8. Yang, S., Li, C., Mei, Y., Liu, W., Liu, R., Chen, W., Han, D., and Xu, K. (2021). Determination of the geographical origin of coffee beans using terahertz spectroscopy combined with machine learning methods. Front. Nutr., 8.10.3389/fnut.2021.680627 ↗
9. Berghian-Grosan, C., and Magdas, D.A. (2020). Application of Raman spectroscopy and machine learning algorithms for fruit distillates discrimination. Sci. Rep., 10.10.1038/s41598-020-78159-8 ↗
10. Geographical origin identification of Chinese red wines using ultraviolet-visible spectroscopy coupled with machine learning techniques10.1016/j.jfca.2023.105265 ↗
11. Yan, X., Xie, Y., Chen, J., Yuan, T., Leng, T., Chen, Y., Xie, J., and Yu, Q. (2022). NIR spectrometric approach for geographical origin identification and taste related compounds content prediction of Lushan Yunwu tea. Foods, 11.10.3390/foods11192976 ↗
12. Rapid identification of rice geographical origin and adulteration by excitation-emission matrix fluorescence spectroscopy combined with chemometrics based on fluorescence probe10.1016/j.foodcont.2022.109547 ↗
13. Cultivar and geographical origin authentication of Italian extra virgin olive oil using front-face fluorescence spectroscopy and chemometrics10.1016/j.foodcont.2020.107604 ↗
14. The use of front face fluorescence spectroscopy to classify the botanical origin of honey samples produced in Switzerland10.1016/j.foodchem.2006.01.039 ↗
15. Spectrofluorimetric, spectrophotometric and chemometric analysis of wheat grains infested by Sitophilus granaries10.1016/j.jspr.2012.04.004 ↗
16. Identification of the geographic origin of peaches by VIS-NIR spectroscopy, fluorescence spectroscopy and image processing technology10.1016/j.jfca.2022.104843 ↗
17. Hou, Z., Jin, Y., Gu, Z., Zhang, R., Su, Z., and Liu, S. (2024). 1H NMR spectroscopy combined with machine-learning algorithm for origin recognition of Chinese famous green tea Longjing tea. Foods, 13.10.3390/foods13172702 ↗
18. Hyperspectral imaging combined with convolutional neural network for Pu’er ripe tea origin recognition10.1016/j.jfca.2024.107093 ↗
19. Intelligent analysis of excitation-emission matrix fluorescence fingerprint to identify and quantify adulteration in camellia oil based on machine learning10.1016/j.talanta.2022.123733 ↗
20. A rapid and low-cost method for detection of nine kinds of vegetable oil adulteration based on 3-D fluorescence spectroscopy10.1016/j.lwt.2023.115419 ↗
21. Hu, Y., Wei, C., Wang, X., Wang, W., and Jiao, Y. (2025). Using three-dimensional fluorescence spectroscopy and machine learning for rapid detection of adulteration in camellia oil. Spectrochim. Acta Part A Mol. Biomol. Spectrosc., 329.10.1016/j.saa.2024.125524 ↗
22. Lia, F., Formosa, J.P., Zammit-Mangion, M., and Farrugia, C. (2020). The first identification of the uniqueness and authentication of Maltese extra virgin olive oil using 3D-fluorescence spectroscopy coupled with multi-way data analysis. Foods, 9.10.3390/foods9040498 ↗
23. Front-face synchronous fluorescence spectroscopy: A rapid and non-destructive authentication method for Arabica coffee adulterated with maize and soybean flours10.1007/s00003-022-01396-8 ↗
24. Fluorescence fingerprints of Sidr honey in comparison with uni/polyfloral honey samples10.1007/s00217-020-03536-6 ↗