journal article·2024-05-10Open access

GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

Ramon Dolcet-Sanjuan, Maria Casanovas, Sandra Franquesa, Eva Alsina, Francisca Carrasco-Cuello, Estanis Torres, Josep Rufat, Cristina Solsona, Neus Teixido

Applied Sciences· Volume 14· Issue 10

Abstract

The temporary immersion system bioreactor named GreenTray® presents advantages over the existing ones. Firstly, there is no need to use forceps to move the shoots or plantlets in or out of the recipient, nor is the use of a scalpel necessary to divide them. Secondly, the basis of the shoot can remain in the GreenTray® and can sprout again over several cycles of growth. These two characteristics reduce the costs in the process of micropropagation. In addition, shoot or plantlet development is enhanced by the aeration of the recipient, thus enriching the recipient’s atmospheric CO2. This article describes and provides images and values of interest in (1) the micropropagation process of some commercial Prunus or Pyrus rootstocks; (2) the plantlet growth under autotrophic conditions; and (3) the inoculation of plants with pathogens that reproduce in vitro the symptoms observed in vivo, using the GreenTray® bioreactor.
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Research context

OpenAlex topics: Plant tissue culture and regeneration · Plant Pathogens and Fungal Diseases · Plant Pathogens and Resistance

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Plant in Vitro Culture Laboratory, Fruticulture Program, IRTA, Fruitcentre, Parc AgroBiotech, 25003 Lleida, Spain

Fruticulture Program, IRTA, Fruitcentre, Parc AgroBiotech, 25003 Lleida, Spain

Efficient Use of Water in Agriculture Program, IRTA, Fruitcentre, Parc AgroBiotech, 25003 Lleida, Spain

Postharvest Program, IRTA, Fruitcentre, Parc AgroBiotech, 25003 Lleida, Spain

Funding

CERCA Programme/Generalitat de Catalunya · DACAAR · Ministerio de Ciencia e Innovación

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