The Antifungal Effect of Weissella confusa WIKIM51 (Wilac D001) on Vaginal Epithelial Cells Infected by Candida albicans
Source abstract
Abstract
Vulvovaginal candidiasis (VVC) is a genital infection caused by Candida albicans (C. albicans). Weissella confusa WIKIM51 (Wilac D001) is known to be detected in dandelion kimchi, produce lactic acid, and have an anti−inflammatory ability; however, its diverse antifungal effects have not been studied. Here, we investigated the antifungal effect of Wilac D001 in C. albicans compared to Lactobacillus species on vaginal epithelial cells (VECs). To test the antifungal ability of Wilac D001 against C. albicans on VECs, an adhesion test, pro-inflammatory cytokines (interleukin (IL)-6 and IL-8) analysis, and a disk diffusion test were performed. The acid tolerance test was conducted to investigate the viability of Wilac D001 in various acidic conditions. Lactobacillus reuteri (L. reuteri) and L. rhamnosus were used as positive controls. Wilac D001 showed the capacity to inhibit the colonization of C. albicans by adhering to VECs, with an inhibitory effect similar to that of positive controls. Both pro−inflammatory cytokines including IL−6 and IL−8 concentrations were significantly decreased when Wilac D001 was treated on C. albicans-infected VECs, respectively (p < 0.001). The result of the disk diffusion test indicates that the inhibitory ability of Wilac D001 is comparable to L. reuteri and L. rhamnosus on agar plates infected with C. albicans. Our results demonstrate that Weissella confusa WIKIM51 has antifungal effects against VECs infected by C. albicans.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Reproductive tract infections research · Probiotics and Fermented Foods · Urinary Tract Infections Management
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Graduate Program in System Health Science and Engineering, Ewha Womans University, Seoul 03760, Republic of Korea
Department of Obstetrics and Gynecology, Ewha Medical Research Institute, College of Medicine, Ewha Womans University, Seoul 07984, Republic of Korea
Pharmsville Co., Ltd., Seoul 06642, Republic of Korea
Funding
National Research Foundation of Korea · Ministry of Education (MOE, Korea), and the National Research Foundation of Korea · Ministry of Health & Welfare
References (45)
Show source-provided references
1. Yano, J., Sobel, J.D., Nyirjesy, P., Sobel, R., Williams, V.L., Yu, Q., Noverr, M.C., and Fidel, P.L. (2019). Current patient perspectives of vulvovaginal candidiasis: Incidence, symptoms, management and post-treatment outcomes. BMC Womens Health, 19.10.1186/s12905-019-0748-8 ↗
2. Vulvovaginal candidiasis: Epidemiologic, diagnostic, and therapeutic considerations10.1016/S0002-9378(98)80001-X ↗
3. Biofilms and vulvovaginal candidiasis10.1016/j.colsurfb.2018.11.011 ↗
4. Lactobacillus species as biomarkers and agents that can promote various aspects of vaginal health10.3389/fphys.2015.00081 ↗
5. O’Hanlon, D.E., Come, R.A., and Moench, T.R. (2019). Vaginal pH measured in vivo: Lactobacilli determine pH and lactic acid concentration. BMC Microbiol., 19.10.1186/s12866-019-1388-8 ↗
6. Antibiotic-associated vulvovaginal candidiasis10.1007/s11908-003-0091-x ↗
7. Risk factors for Candida vulvovaginitis10.1097/00006254-199208000-00015 ↗
8. Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion10.1186/s12934-020-01390-5 ↗
9. Efficacy and safety of Lactobacillus plantarum P 17630 strain soft vaginal capsule in vaginal candidiasis: A randomized non-inferiority clinical trial
10. Primary metabolite kinetics of bacteriocin biosynthesis by Lactobacillus amylovorus and evidence for stimulation of bacteriocin production under unfavourable growth conditions10.1099/00221287-142-4-817 ↗
11. The vaginal microenvironment: The physiologic role of lactobacilli10.3389/fmed.2018.00181 ↗
12. Enhancement of Microbiota in Healthy Macaques Results in Beneficial Modulation of Mucosal and Systemic Immune Function10.4049/jimmunol.1502470 ↗
13. Improved treatment of vulvovaginal candidiasis with fluconazole plus probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-1410.1111/j.1472-765X.2008.02477.x ↗
14. Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates10.1111/jam.12772 ↗
15. Probiotic Interference of Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 with the Opportunistic Fungal Pathogen Candida albicans
16. Taxonomic studies on some leuconostoc-like organisms from fermented sausages: Description of a new genus Weissella for the Leuconostoc paramesenteroides group of species10.1111/j.1365-2672.1993.tb01600.x ↗
17. Novel Leuconostoc citreum starter culture system for the fermentation of kimchi, a fermented cabbage product10.1023/A:1026050410724 ↗
18. Park, S.M., Mok, J.Y., Cho, H.-R., Song, I.-b., Shin, Y.J., Lee, K.b., Lee, B.W., and Bae, M.-J. (2021). Effects of Weissella confusa Wilac D001 Isolated from Dandelion Kimchi on Dextran Sulphate Sodium-Induced Colitis in Mice. Food Suppl. Biomater. Health, 1.10.52361/fsbh.2021.1.e25 ↗
19. Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children’s feces10.1186/s12934-019-1158-1 ↗
20. Genomics of Weissella cibaria with an examination of its metabolic traits10.1099/mic.0.000053 ↗
21. Vaginal microbiome profiles of pregnant women in Korea using a 16S metagenomics approach10.1111/aji.13124 ↗
22. Polysaccharides of Weissella cibaria Act as a Prebiotic to Enhance the Probiotic Potential of Lactobacillus rhamnosus10.1007/s12010-022-04104-2 ↗
23. Weissella cibaria WIKIM28 ameliorates atopic dermatitis-like skin lesions by inducing tolerogenic dendritic cells and regulatory T cells in BALB/c mice10.1038/srep40040 ↗
24. Defined anaerobic growth medium for studying Candida albicans basic biology and resistance to eight antifungal drugs10.1128/AAC.48.7.2350-2354.2004 ↗
25. Assessment of the capacity of Lactobacillus to inhibit the growth of uropathogens and block their adhesion to vaginal epithelial cells10.1086/318070 ↗
26. Activated lactoferrin’s ability to inhibit Candida growth and block yeast adhesion to the vaginal epithelial monolayer
27. Influence of probiotic vaginal lactobacilli on in vitro adhesion of urogenital pathogens to vaginal epithelial cells10.1111/j.1472-765X.2006.01934.x ↗
28. Lactobacillus crispatus Modulates Vaginal Epithelial Cell Innate Response to Candida albicans10.4103/0366-6999.198927 ↗
29. Matevosyan, L., Bazukyan, I., and Trchounian, A. (2019). Antifungal and antibacterial effects of newly created lactic acid bacteria associations depending on cultivation media and duration of cultivation. BMC Microbiol., 19.10.1186/s12866-019-1475-x ↗
30. Vaginal microbiome signature is associated with spontaneous preterm delivery10.3389/fmed.2019.00201 ↗
31. Elizaldi, S.R., Verma, A., Walter, K.A., Rolston, M., Dinasarapu, A.R., Durbin-Johnson, B.P., Settles, M., Kozlowski, P.A., Raeman, R., and Iyer, S.S. (2019). Rectal microbiome composition correlates with humoral immunity to HIV-1 in vaccinated rhesus macaques. Msphere, 4.10.1128/mSphere.00824-19 ↗
32. Altered host-microbe interaction in HIV: A target for intervention with pro- and prebiotics10.3109/08830185.2010.505310 ↗
33. Meningitis due to Weissella confusa10.1016/j.ajem.2020.02.004 ↗
34. Safety assessment of Weissella confusa–A direct-fed microbial candidate10.1016/j.yrtph.2019.104414 ↗
35. Ahmed, S., Singh, S., Singh, V., Roberts, K.D., Zaidi, A., and Rodriguez-Palacios, A. (2022). The Weissella genus: Clinically treatable bacteria with antimicrobial/probiotic effects on inflammation and cancer. Microorganisms, 10.10.3390/microorganisms10122427 ↗
36. Fatmawati, N.N.D., Gotoh, K., Mayura, I.P.B., Nocianitri, K.A., Suwardana, G.N.R., Komalasari, N.L.G.Y., Ramona, Y., Sakaguchi, M., Matsushita, O., and Sujaya, I.N. (2020). Enhancement of intestinal epithelial barrier function by Weissella confusa F213 and Lactobacillus rhamnosus FBB81 probiotic candidates in an in vitro model of hydrogen peroxide-induced inflammatory bowel disease. BMC Res. Notes, 13.10.1186/s13104-020-05338-1 ↗
37. Mixed biofilms of pathogenic Candida-bacteria: Regulation mechanisms and treatment strategies10.1080/1040841X.2021.1921696 ↗
38. A review of cell adhesion studies for biomedical and biological applications10.3390/ijms160818149 ↗
39. Adhesion of human probiotic Lactobacillus rhamnosus to cervical and vaginal cells and interaction with vaginosis-associated pathogens10.1155/2008/549640 ↗
40. Cytokine and chemokine production by human oral and vaginal epithelial cells in response to Candida albicans10.1128/IAI.70.2.577-583.2002 ↗
41. Vaginal IL-8 levels are positively associated with Candida albicans and inversely with lactobacilli in HIV-infected women10.1016/j.jri.2007.11.001 ↗
42. Weakly acidic pH reduces inflammatory cytokine expression in airway epithelial cells10.1186/s12931-016-0399-3 ↗
43. Methods for in vitro evaluating antimicrobial activity: A review10.1016/j.jpha.2015.11.005 ↗
44. Effect of Antibiotics on Vulvovaginal Candidiasis: A MetroNet Study10.3122/jabfm.2008.04.070169 ↗
45. Investigation of antibacterial, acid and bile tolerance properties of lactobacilli isolated from Koozeh cheese