journal article·2023-07-27Open access

EEM Fluorescence Spectroscopy Coupled with HPLC-DAD Analysis for the Characterization of Bud Derivative Dietary Supplements: A Preliminary Introduction to GEMMAPP, the Free Data-Repository from the FINNOVER Project

Federica Turrini, Dario Donno, Federica Grasso, Eleonora Mustorgi, Gabriele Loris Beccaro, Maddalena Guido, Teresa Fior, Massimo Grilli, Anna Pittaluga, Raffaella Boggia

Applied Sciences· Volume 13· Issue 15

Abstract

Bud derivatives (BDs) represent a category of botanicals obtained by macerating the meristematic tissues (buds or young sprouts) of plants; widely used since ancient times in complementary medicine, they remain poorly investigated to date. In this study, a contribution is made toward the identification of the correct “a posteriori” attribution of the botanical species in commercial BDs, which is very important for ensuring the quality and safety of these natural products. Excitation–emission matrix (EEM) fluorescence spectroscopy proved to be a rapid, non-destructive and low-cost analytical instrument for providing a preliminary qualitative characterization of the BDs, allowing for the identification of specific spectral regions related to flavonol compounds and cinnamic and benzoic acids, in agreement with the targeted chromatographic analysis (HPLC-DAD), which completely defined a phytochemical profile for each BD considered. This information will be implemented in the first web application for the recognition of vegetable buds, named GEMMAPP, which was designed by the Authors and is proposed as a tool and future scientific data repository for providing information about the main, typical BDs of the France–Italy Alcotra territory.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Phytochemicals and Antioxidant Activities · Fermentation and Sensory Analysis · Phytochemistry and Biological Activities

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Pharmacy, University of Genoa, Viale Cembrano 4, 16148 Genoa, Italy

National Center for the Development of New Technologies in Agriculture (Agritech), 80121 Napoli, Italy

Department of Agriculture, Forestry and Food Science, University of Torino, Largo Braccini 2, Grugliasco, 10095 Turin, Italy

Geal Pharma, Strada Rivà 20, Bricherasio, 10060 Turin, Italy

ERREEFFE, Via Acquarone, 16125 Genoa, Italy

National Biodiversity Future Center (NBFC), 90133 Palermo, Italy

Funding

European Union · Project National Center for the Development of New Technologies in Agriculture (Agritech) · Italian Ministry of University and Research

References (38)

Show source-provided references
1. (2021, April 02). FINNOVER Interreg Alcotra Project 2017–2020. Available online: http://www.interreg-finnover.com/.
2. Turrini, F., Donno, D., Beccaro, G.L., Pittaluga, A., Grilli, M., Zunin, P., and Boggia, R. (2020). Bud-derivatives, a novel source of polyphenols and how different extraction processes affect their composition. Foods, 9.10.3390/foods9101343 ↗
3. Pharmacopée Française (2023, March 21). Available online: http://ansm.sante.fr/Mediatheque/Publications/Pharmacopee-francaise-Plan-Preambule-index.
4. Donno, D., Mellano, M.G., Cerutti, A.K., and Beccaro, G.L. (2016). Biomolecules and Natural Medicine Preparations: Analysis of New Sources of Bioactive Compounds from Ribes and Rubus spp. Buds. Pharmaceuticals, 9.10.3390/ph9010007 ↗
5. Rao, A.V., and Rao, L.G. (2015). Phytochemicals—Isolation, Characterisation and Role in Human Health, InTech. [1st ed.].10.5772/58648 ↗
6. An innovative green extraction and re-use strategy to valorize food supplement by-products: Castanea sativa bud preparations as case study10.1016/j.foodres.2018.12.018 ↗
7. Bud extracts from Tilia tomentosa Moench inhibit hippocampal neuronal firing through GABAA and benzodiazepine receptors activation10.1016/j.jep.2015.06.016 ↗
8. Bud extracts from Salix caprea L. inhibit voltage gated calcium channels and catecholamines secretion in mouse chromaffin cells10.1016/j.phymed.2017.09.006 ↗
9. Turrini, F., Vallarino, G., Cisani, F., Donno, D., Beccaro, G.L., Zunin, P., Boggia, R., Pittaluga, A., and Grilli, M. (2020). Use of an Animal Model to Evaluate Anxiolytic Effects of Dietary Supplementation with Tilia tomentosa Moench Bud Extracts. Nutrients, 12.10.3390/nu12113328 ↗
10. Lakowicz, J.R. (2007). Principles of Fluorescence Spectroscopy, Kluwer Academic/Plenum. [3rd ed.].10.1007/978-0-387-46312-4 ↗
11. Barbieri, D., Gabriele, M., Summa, M., Colosimo, R., Leonardi, D., Domenici, V., and Pucci, L. (2020). Antioxidant, Nutraceutical Properties, and Fluorescence Spectral Profiles of Bee Pollen Samples from Different Botanical Origins. Antioxidants, 9.10.3390/antiox9101001 ↗
12. Varietal classification of white wines by fluorescence spectroscopy10.1007/s13197-020-04291-y ↗
13. Sikorska, E., Nowak, P., Pawlak-Lemańska, K., and Sikorski, M. (2022). Characterization and Classification of Direct and Commercial Strawberry Beverages Using Absorbance–Transmission and Fluorescence Excitation–Emission Matrix Technique. Foods, 11.10.3390/foods11142143 ↗
14. (2023, March 20). GEMMAPP. Available online: http://www.gemmapp.it/.
15. Donno, D., Turrini, F., Boggia, R., Guido, M., Gamba, G., Mellano, M.G., Riondato, I., and Beccaro, G.L. (2020). Sustainable Extraction and Use of Natural Bioactive Compounds from the Waste Management Process of Castanea spp. Bud-Derivatives: The FINNOVER Project. Sustainability, 12.10.3390/su122410640 ↗
16. Pulsed Ultrasound-Assisted Extraction as an Alternative Method to Conventional Maceration for the Extraction of the Polyphenolic Fraction of Ribes nigrum Buds: A New Category of Food Supplements Proposed by The FINNOVER Project10.3390/foods8100466 ↗
17. Common ash (Fraxinus excelsior L.) seeds as a new vegetable oil source10.1016/j.lwt.2020.109811 ↗
18. Fraxinus excelsior seed extract FraxiPureTM limits weight gains and hyperglycemia in high-fat diet-induced obese mice10.1016/j.phymed.2010.09.010 ↗
19. Acute effects of Fraxinus excelsior L. seed extract on postprandial glycemia and insulin secretion on healthy volunteers10.1016/j.jep.2009.08.039 ↗
20. Eaton, E.G.S.D.J., Caudullo, G., Oliveira, S., and De Rigo, D. (2016). European Atlas of Forest Tree Species, Public Office of the European Union.
21. Metabolite profiling of ‘green’ extracts of Corylus avellana leaves by 1H NMR spectroscopy and multivariate statistical analysis10.1016/j.jpba.2018.07.046 ↗
22. Cyclic Diarylheptanoids from Corylus avellana Green Leafy Covers: Determination of Their Absolute Configurations and Evaluation of Their Antioxidant and Antimicrobial Activities10.1021/acs.jnatprod.6b00703 ↗
23. Castanea spp. buds as a phytochemical source for herbal preparations: Botanical fingerprint for nutraceutical identification and functional food standardization10.1002/jsfa.6627 ↗
24. Ficus carica L. (Moraceae): An ancient source of food and health10.1016/j.foodchem.2014.04.112 ↗
25. Characterization of steam volatiles in the essential oil of black currant buds and the antioxidant properties of different bud extracts10.1021/jf7037168 ↗
26. Antioxidant and anti-inflammatory activities of Ribes nigrum extracts10.1016/j.foodchem.2011.09.076 ↗
27. Botanicals in Ribes nigrum bud-preparations: An analytical fingerprinting to evaluate the bioactive contribution to total phytocomplex10.3109/13880209.2013.786101 ↗
28. Chemical information of Chinese medicines: A challenge to chemist10.1016/j.chemolab.2005.05.006 ↗
29. Donno, D., Mellano, M.G., Gamba, G., Riondato, I., and Beccaro, G.L. (2020). Analytical strategies for fingerprinting of antioxidants, nutritional substances, and bioactive compounds in foodstuffs based on high performance liquid chromatography–mass spectrometry: An overview. Foods, 9.10.3390/foods9121734 ↗
30. Multiple chromatographic fingerprinting and its application to the quality control of herbal medicines10.1016/j.aca.2005.09.037 ↗
31. Hplc fingerprint analysis combined with chemometrics for pattern recognition of ginger10.3109/13880209.2013.837493 ↗
32. Analytical fingerprint and chemometrics as phytochemical composition control tools in food supplement analysis: Characterization of raspberry bud-preparations of different cultivars10.1002/jsfa.7494 ↗
33. Feasibility of UV–VIS-Fluorescence Spectroscopy combined with pattern recognition techniques to authenticate a new category of plant food supplements10.1007/s13197-017-2684-7 ↗
34. Fluorescence spectroscopy and multy-way techniques. PARAFAC10.1039/c3ay41160e ↗
35. Explorative study of apple juice fluorescence in relation to antioxidant properties10.1016/j.foodchem.2016.05.007 ↗
36. Separation of phenolic compounds by high-performance liquid chromatography with absorbance and fluorimetric detection10.1016/S0021-9673(01)00598-2 ↗
37. Combining excitation-emission matrix fluorescence spectroscopy, parallel factor analysis, cyclodextrin-modified micellar electrokinetic chromatography and partial least squares class-modelling for green tea characterization10.1016/j.jpba.2018.07.001 ↗
38. Phenolic profiling of 12 strawberry cultivars using different spectroscopic methods10.1021/acs.jafc.9b07331 ↗