UHPLC-MS Method for the Analysis of the Molecular Adjuvant Sulfavant A
Source abstract
Abstract
A fast and sensitive method that is based on Ultra High Performance Liquid Chromatography coupled with High Resolution Mass Spectrometry (UHPLC-HRMS) for the measurement of Sulfavant A, a molecular adjuvant with a sulfolipid skeleton, is described. The method has been validated over the linearity range of 2.5–2000 ngmL−1 using a deuterated derivative (d70-Sulfavant A) as internal standard. Chromatographic separation is based on a UHPLC Kinetex® 2.6 µm PS C18 column and a gradient of methanol in 0.32 mM ammonium hydroxide solution buffered at pH 8. The lowest limit of quantification of Sulfavant A was 6.5 ngmL−1. The analytical procedure was tested on an extract of mice lung spiked with 30, 300, and 1500 ng of Sulfavant A. The analysis revealed a precision and accuracy value (as a mean value of all the quality control samples analyzed) of 4.7% and 96% in MeOH and 6.4% and 93.4% in the lung extracts, respectively.
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Research context
OpenAlex topics: Nitric Oxide and Endothelin Effects · Antibiotics Pharmacokinetics and Efficacy · Eicosanoids and Hypertension Pharmacology
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Bio-Organic Chemistry Unit, National Research Council-Institute of Bio-Molecular Chemistry (ICB-CNR), Via Campi Flegrei 34, 80078 Pozzuoli, Italy
BioSearch Srl., Villa Comunale c/o Stazione Zoologica “A.Dohrn”, 80121 Napoli, Italy
Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy
Department of Biology, University of Napoli “Federico II”, Via Cupa Nuova Cinthia 21, 80126 Napoli, Italy
Funding
POR Campania FESR 2014-2020
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