Production of Alternative Fat from Adipose-Derived Stem Cell from Bovine in 3D Culture
Source abstract
Abstract
Cultivated meat, developed through cell culture technology, is emerging as a promising solution that closely mimics both the flavor and nutrient profiles of conventional meat. One key component that contributes to the flavor of meat is fat content. In this study, bovine adipose-derived stem cells (bADSCs) were cultured for the production of alternative fat in vitro. The expression of mesenchymal stem cell (MSC) markers (CD29, CD73, and CD105) and colony forming efficiency were assessed to characterize bADSCs. bADSCs were differentiated into adipocytes to produce cultivated fat in 2D or 3D culture. The cultivated fat was analyzed by gas chromatography to verify the similarity of the fatty acids of animal-derived fat. Our results show that bADSCs have characteristics of MSC and could differentiate into adipocyte. The ratio of unsaturated fatty acids and saturated fatty acids in cultivated fat and adipose tissue was similar. Adipogenic differentiation of ADSCs using a textured vegetable protein (TVP) scaffold could form the lipid droplets in the TVP. This study demonstrated the establishment of a culture system for the fat production from bADSCs in vitro. The fat produced through bADSCs shows the potential to be used in the composition of hybrid-cultivated meat.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Agriculture Sustainability and Environmental Impact
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Research Institute, Simple Planet Co., Ltd., Seoul 04790, Republic of Korea
Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea
Digital OMICs Research Center, Korea Basic Science Institute (KBSI), Chungbuk 28119, Republic of Korea
Funding
Simple Planet · Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) · Korea Institute for Advancement of Technology (KIAT) · Ministry of SMEs and Startups · Korea Basic Science Institute
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