Biomass and Phenolic Acid Accumulation in Salvia austriaca Hairy Roots Grown in Temporary Immersion and Mist-Trickling Bioreactors
Source abstract
Abstract
Transformed roots of Salvia austriaca were cultivated for 45 days in various systems, including Erlenmeyer flasks, a temporary immersion system (TIS) bioreactor, and a mist-trickling bioreactor, under controlled light conditions. The mist-trickling bioreactor yielded the highest biomass, with fresh and dry weights of 155.4 g/L and 10.2 g/L, respectively. Quantitative UHPLC analysis of hydromethanolic extracts revealed the biosynthesis of significant phenolic acids: caffeic acid, rosmarinic acid, and salvianolic acid A. Among these, rosmarinic acid was the most abundant, with its concentration varying based on the cultivation system. The highest total phenolic acid content, 165 mg/L, was obtained in the mist-trickling bioreactor, demonstrating its superiority in both biomass production and phenolic acid biosynthesis. This study highlights the potential of mist-trickling bioreactors for optimizing growth and metabolite production in S. austriaca transformed root cultures.
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Research context
OpenAlex topics: Polysaccharides and Plant Cell Walls · Fungal Biology and Applications · Plant tissue culture and regeneration
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, 1 Muszyńskiego St., 90-151 Lodz, Poland
Department of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland
Department of Medical Rehabilitation, Faculty of Health Sciences, Medical University of Lodz, 251 Pomorska St., 92-213 Lodz, Poland
National Seminar for Older Candidates Ordination in Lodz, 14 Saint Stanislaw Kostka St., 90-457 Lodz, Poland
Department of Pharmacology and Therapeutics, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala P.O. Box 7062, Uganda
Funding
Medical University of Lodz
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