Molecular Characterization of Isolates of the Banana Bunchy Top Virus (BBTV) from the District of Chókwè, Mozambique
Source abstract
Abstract
Banana bunchy top virus (BBTV) was recently detected in Mozambique and appears to be limited to the provinces of Gaza, Maputo and Zambezia, but it has great potential to spread to other provinces. Despite its importance, nothing is known about the BBTV isolates that occur in Mozambique. In this study, the sequences of the S and R genes of forty isolates chosen as representatives of samples collected previously from eleven farms of the four administrative posts of the district of Chóckwè, province of Gaza, were sequenced and analyzed. The S-DNA nucleotide sequences of the analyzed isolates were highly conserved, with identity ranging from 97% to 100%. The same was observed for the R-DNA sequences, with most identities ranging between 98% and 100% among the isolates from Chókwè and above 90% when compared to the isolates from GenBank. The phylogenetic analysis showed that the Mozambican BBTV isolates belong to the Pacific–Indian Oceans (PIO) group, showing greater proximity to the isolate JQ820453 from Malawi than to the isolates from sub-Saharan countries, which were grouped in a distinct subclade. This is the first study conducted to determine the molecular characteristics of BBTV isolates present in Mozambique.
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Research context
OpenAlex topics: Plant Virus Research Studies · Transgenic Plants and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Research Department in Agricultural Sciences, Pedagogical University of Maputo (UP-Maputo), João Carlos Raposo Beirão Street, Number 135, Maputo P.O. Box 3276, Mozambique
Department of Plant Pathology, Federal University of Lavras, P.O. Box 3037, Lavras CEP 37203-202, MG, Brazil
Funding
National Council for Scientific and Technological Development (CNPq) · Coordination for the Improvement of Higher Education Personnel (CAPES) · Foundation for Research Support of the State of Minas Gerais (FAPEMIG)
References (49)
Show source-provided references
1. Potential of cooking bananas in addressing food security in East Africa
2. Multidisciplinary perspectives on banana (Musa spp.) domestication10.1073/pnas.1102001108 ↗
3. Sharrock, S., and Frison, E.A. (1998). Networking Banana and Plantain: INIBAP Annual Report, Network for the Improvement of Banana and Plantain.
4. Overview of the Current Status of Uganda’s Banana Sector: Formalizing the Matooke Sector may not be the Best Policy Option10.2174/0118743315252945231106071452 ↗
5. Pro-vitamin A carotenoids in East African highland banana and other Musa cultivars grown in Uganda10.1002/fsn3.1308 ↗
6. Chabi, M.C., Dassou, A.G., Dossou-Aminon, I., Ogouchoro, D., Aman, B.O., and Dansi, A. (2018). Banana and plantain production systems in Benin: Ethnobotanical investigation, varietal diversity, pests, and implications for better production. J. Ethnobiol. Ethnomed., 14.10.1186/s13002-018-0280-1 ↗
7. MADER—Ministério de Agricultura e Desenvolvimento (2018). Rural Indústria da Banana em Moçambique—Situação de Foc TR4 e BBTV.
8. Banana bunchy top virus in sub-Saharan Africa: Investigations on virus distribution and diversity10.1016/j.virusres.2011.04.021 ↗
9. First Report of Banana bunchy top virus in Banana (Musa spp.) from South Africa10.1094/PDIS-12-15-1422-PDN ↗
10. (2022, July 19). ICTV—International Committee on Taxonomy of Viruses (2021) International Committee on Taxonomy of Viruses 9th Report. Available online: https://ictv.global/report_9th/ssDNA/Nanoviridae.
11. Characterisation of Rep-encoding components associated with banana bunchy top nanovirus in Vietnam10.1007/s007050200019 ↗
12. Use of polymerase chain reaction (PCR) to study transmission of banana bunchy top virus by the banana aphid (Pentalonia nigronervosa)10.1111/j.1744-7348.1996.tb07089.x ↗
13. The genome organization of banana bunchy top virus: Analysis of six ssDNA components10.1099/0022-1317-76-6-1471 ↗
14. An iterated sequence in the genome of Banana bunchy top virus is essential for efficient replication10.1099/vir.0.82166-0 ↗
15. Additional Rep-encoding DNAs associated with banana bunchy top virus10.1007/s007050170192 ↗
16. Ji, X., Yu, N., Qu, L., Li, B., and Liu, Z. (2019). Banana bunchy top virus (BBTV) nuclear shuttle protein interacts and re-distributes BBTV coat protein in Nicotiana benthamiana. 3 Biotech, 9.10.1007/s13205-019-1656-1 ↗
17. Identification of a major pathogenicity determinant and suppressors of RNA silencing encoded by a South Pacific isolate of Banana bunchy top virus originating from Pakistan10.1007/s11262-010-0559-3 ↗
18. Yu, N.T., Xie, H.M., Zhang, Y.L., Wang, J.H., Xiong, Z., and Liu, Z.X. (2019). Independent modulation of individual genomic component transcription and a cis-acting element related to high transcriptional activity in a multipartite DNA virus. BMC Genom., 20.10.1186/s12864-019-5901-0 ↗
19. Cleavage of the Babuvirus Movement Protein B4 into Functional Peptides Capable of Host Factor Conjugation is Required for Virulence10.1007/s12250-019-00094-4 ↗
20. Cloning and sequence analysis of two Banana bunchy top virus genomes in Hainan10.1007/s11262-012-0718-9 ↗
21. Evidence for two groups of Banana bunchy top virus isolates10.1099/0022-1317-75-12-3541 ↗
22. UNDP—United Nations Development Programme (2007). MDG Achievement Fund—Environment and Climate Change Thematic Window, MDG-F. Available online: https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.462.1402&rep=rep1&type=pdf.
23. A simple and efficient method for DNA extraction from grapevine cultivars andVitis species10.1007/BF02668658 ↗
24. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets10.1093/molbev/msw054 ↗
25. Characterization and genetic status of Banana bunchy top virus isolated from Okinawa, Japan10.1007/s10327-004-0162-3 ↗
26. Genetic diversity and possible evidence of recombination among Banana Bunchy Top Virus (BBTV) isolates
27. James, A.P. (2011). Viruses of Banana in East Africa. [Ph.D. Thesis, Queensland University of Technology].
28. Molecular characterisation of Banana bunchy top virus (BBTV) from Pakistan10.1007/s11262-007-0168-y ↗
29. The global distribution of Banana bunchy top virus reveals little evidence for frequent recent, human-mediated long distance dispersal events10.1093/ve/vev009 ↗
30. Abacá bunchy top virus, a new member of the genus Babuvirus (family Nanoviridae)10.1007/s00705-007-1077-z ↗
31. James, A.P., Soko, M.M., Mugini, J.A., Geijskes, R.J., Dale, J.L., and Harding, R.M. (2021, July 15). Molecular Characterisation of Banana Bunchy Top Virus Isolates from Malawi and Rwanda. Centre for Tropical Crops and Biocommodities, Available online: https://www.ncbi.nlm.nih.gov/nuccore/394326320.
32. Cloning and sequencing of DNA component 1 of two BBTV strains
33. Kawabe, K., and Onuki, M. (2023, January 10). Banana Bunchy Top Virus Coat Protein (CP) Gene, Available online: https://www.ncbi.nlm.nih.gov/nuccore/AB078023.1/.
34. Recent distribution and diversity analysis on banana bunchy top virus of banana and alternative host in Indonesia10.1007/s40858-021-00443-3 ↗
35. Furuya, N., and Natsuaki, K.T. (2023, January 21). Identification of Banana Viruses in Myanmar, Available online: https://www.ncbi.nlm.nih.gov/nuccore/AB252644.
36. Bashir, S., Naqvi, S.M., Muhammad, A., Hussain, I., Ali, K., Khan, M.R., Farrakh, S., Yasmin, T., and Hyder, M.Z. (2022). Banana bunchy top virus genetic diversity in Pakistan and association of diversity with recombination in its genomes. PLoS ONE, 17.10.1371/journal.pone.0263875 ↗
37. First Report of Banana bunchy top virus in Banana and Plantain (Musa spp.) in Nigeria10.1094/PDIS-08-12-0745-PDN ↗
38. Occurrence of Banana Bunchy Top Disease Caused by the Banana bunchy top virus on Banana and Plantain (Musa sp.) in Cameroon10.1094/PDIS-93-10-1076C ↗
39. Karan, M. (1995). Sequence Diversity of DNA Fragments Associated with Banana Bunchy Top Virus. [Ph.D. Thesis, Queensland University of Technology].
40. Molecular comparative analysis of component 1 (DNA-R) of an Egyptian isolate of banana bunchy top nanovirus isolated from banana aphid (Pentalonia nigronervosa)10.1016/j.jgeb.2012.05.003 ↗
41. Karan, M., Harding, R.M., and Dale, J.L. (1997). Association of Banana bunchy top virus DNA components 2 to 6 with bunchy top disease. Mol. Plant Pathol., 1–7.
42. Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa10.5897/AJAR2014.8862 ↗
43. Sequence variability in the coat protein gene of two groups of banana bunchy top isolates10.1007/s007050050049 ↗
44. Banana Bunchy Top: An Economically Important Tropical Plant Virus Disease10.1016/S0065-3527(08)60321-8 ↗
45. Note sur l’apparition au Gabon d’une grave maladie virale des bananiers et plantains: Le bunchy top
46. Wardlaw, C.W. (1961). Banana Diseases Including Plantain and Abaca, Longmans, Green.
47. Gowen, S. (1995). Bananas and Plantains, Chapman and Hall.10.1007/978-94-011-0737-2 ↗
48. EPPO—European and Mediterranean Plant Protection Organization (2021, July 15). Global Database. Banana Bunchy Top Vírus (BBTV00). Available online: https://gd.eppo.int/taxon/BBTV00/distribution.
49. Monitoring the distribution of Banana bunchy top virus in South Africa: A country-wide survey10.1007/s00705-022-05451-5 ↗