Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment
Source abstract
Abstract
In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.
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Research context
OpenAlex topics: Plasma Applications and Diagnostics · Electrospun Nanofibers in Biomedical Applications · Laser Applications in Dentistry and Medicine
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pathophysiology, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia
Institute of Physics, University of Belgrade, 11000 Belgrade, Serbia
Department of Oral Surgery, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia
Department of Surgery, Orthopedy and Ophthalmology, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Serbia
Department of General and Oral Histology, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia
Group for Molecular Oncology, Institute for Medical Research, University of Belgrade, 11000 Belgrade, Serbia
Group for Hematology and Stem Cells, Institute for Medical Research, University of Belgrade, 11000 Belgrade, Serbia
Funding
Ministry of Science, Technological Development and Innovations of the Republic of Serbia
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