In Vitro Genotoxicity Assessment from the Glycyrrhiza New Variety Extract
Source abstract
Abstract
The various species that comprise the genus Glycyrrhiza (Licorice) have long been used as oriental herbal medicines in Asian countries. Wongam (WG), which is a new variety of Glycyrrhiza, was developed in Korea to overcome the limitations of low productivity, environmental restrictions, and an insufficient presence of glycyrrhizic acid and liquiritigenin. In this study, we evaluated WG extract’s genotoxicity through an in vitro bacterial reverse mutation (AMES) test, an in vitro chromosome aberration test, and an in vivo mouse bone marrow micronucleus test. In the AMES test, WG extract at concentrations of up to 5000 µg/plate showed no genotoxicity regardless of S9 mix. No chromosome aberrations appeared after 6 h in 1400 µg/mL WG extract regardless of S9 mix or in 1100 µg/mL WG extract after 24 h without S9 mix. Nor was there a significant increase in the number of micronucleated polychromatic erythrocytes to total erythrocytes up to 5000 mg/kg/day for 2 days detected in the micronucleus test. These results confirm that WG extract is safe for use as an herbal medicine, as it precipitates no detectable genotoxic effects.
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Research context
OpenAlex topics: Pharmacological Effects of Natural Compounds · Phytochemistry and biological activity of medicinal plants · Ginseng Biological Effects and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pharmacy, College of Pharmacy, Jeonbuk National University, 567 Baekje-daero, Jeonju-si 54896, Jeollabuk-do, Korea
Department of Oriental Medicine Resources, Jeonbuk National University, 79 Gobong-ro, Iksan 54596, Jeollabuk-do, Korea
Herbal Crop Research Division, National Institue of Horticultural and Herbal Science, Rural Development Administration, 92 Bisan-ro, Eumseong 27709, Chungbuk, Korea
Cnh Center for Cancer Research, 462 Bongeunsa-ro, Gangnam-gu, Seoul 06154, Korea
Department of Pharmacology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Gangwon-do, Korea
Department of Pharmacy, College of Pharmacy, Dongduk Women’s University, 60 Hwarang-ro 13-gil, Seongbuk-gu, Seoul 02748, Korea
Department of Korean Pharmacy, College of Pharmacy, Woosuk Uninversity, 443 Samrye-ro, Samrye-eup, Wanju-gun 55338, Jeollabuk-do, Korea
Crop Production & Physiology Division, National Institue of Crop Science, Rural Development Administration, 181 Hyeoksin-ro, Wanju 55365, Jeollabuk-do, Korea
Funding
Rural Development Administration
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