Effect of Semi-Continuous Anaerobic Digestion on the Substrate Solubilisation of Lignin-Rich Steam-Exploded Ludwigia grandiflora

In this study, semi-continuous anaerobic digestion of lignin-rich steam-exploded Ludwigia grandiflora (Lignin = 25.22% ± 4.6% total solids) was performed to understand better the effect of steam explosion on the substrate solubilisation and inhibitors formation during the process. Steam explosion pretreatment was performed at 180 °C for 30 min at a severity factor of 3.8 to enhance the biogas yield of the lignocellulosic biomass. The semi-continuous anaerobic digestion was performed in a continuously stirred tank reactor for 98 days at an initial hydraulic retention time of 30 days and an organic loading rate of 0.9 g-VS L−1 day−1. The performed steam explosion pretreatment caused biomass solubilisation, resulting in enhanced biogas production during the process. During the anaerobic digestion process, the average biogas yield was 265 mL g-VS−1, and the pH throughout the operation was in the optimum range of 6.5–8.2. Due to fluctuations in the biogas yield, the hydraulic retention time and organic loading rate were changed on day 42 (50 days and 0.5 g-VS L−1 day−1) and on day 49 (40 days and 0.7 g-VS L−1 day−1), and 1 M of NaOH was added to the liquid fraction of the steam-exploded L. grandiflora during the latter part of the operation to maintain the stability in the reactor. Therefore, the steam explosion pretreatment helped in the degradation of L. grandiflora by breaking the lignocellulose structure. In addition, changes in the operating conditions of the anaerobic digestion led to an increase in the biogas production towards the end of the process, leading to the stability in the CSTR.