journal article·2021-03-01Open access

Sea Bass Side Streams Valorization Assisted by Ultrasound. LC-MS/MS-IT Determination of Mycotoxins and Evaluation of Protein Yield, Molecular Size Distribution and Antioxidant Recovery

Fadila Al Khawli, Noelia Pallarés, Francisco J. Martí-Quijal, Emilia Ferrer, Francisco J. Barba

Applied Sciences· Volume 11· Issue 5

Abstract

Sea bass side streams obtained from the fish industry can be a good source of nutrients such as high-quality protein, lipids, and antioxidants. In this context, it is interesting to develop innovative approaches to extract the added-value compounds from fish side streams. In this study, a strategy to obtain valuable compounds and to minimize the presence of toxins from fish side streams assisted by ultrasound technology is presented. For this purpose, ultrasound-assisted extraction (UAE) conditions have been optimized based on a response surface methodology (RSM) with the dependent variables: time (0.5–30 min), pH (5.5–8.5), and temperature (20–50 °C). After the treatment, protein extraction and antioxidant activity were evaluated in the extracts obtained from sea bass side streams using some spectrophotometric and fluorometric methods. Furthermore, mycotoxin presence was evaluated by LC-MS/MS-QTRAP. The results obtained revealed a high recovery percentage of proteins and antioxidant activity in the UAE extracts, especially those obtained from viscera, when the time and temperature increased to 30 min and 50 °C. Furthermore, none of the analyzed mycotoxins were detected in the sea bass side streams extracts under the studied variables. The experimental values obtained were close to the expected values, confirming the validity of the model employed to establish the optimal UAE conditions.
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Research context

OpenAlex topics: Mycotoxins in Agriculture and Food · Meat and Animal Product Quality · Bee Products Chemical Analysis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, 46100 València, Spain

Funding

Bio-Based Industries Joint Undertaking

References (41)

Show source-provided references
1. (2018). The State of World Fisheries and Aquaculture 2018-Meeting the Sustainable Development Goals, FAO.
2. Fish residue valorisation by the production of value- added compounds towards a sustainable zero waste industry: A critical review
3. Marine peptides: Bioactivities and applications
4. Biochemical and functional properties of sardinella (Sardinetta aurita) by-product hydrolysates
5. Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis10.1016/j.procbio.2007.02.006 ↗
6. Protein hydrolysates from bluefin tuna (Thunnus thynnus) heads as influenced by the extent of enzymatic hydrolysis10.1007/s12257-012-0053-y ↗
7. Marine collagen peptide isolated from Chum Salmon (Oncorhynchus keta) skin facilitates learning and memory in aged C57BL/6J mice10.1016/j.foodchem.2009.04.120 ↗
8. Antioxidant and functional properties of collagen hydrolysates from Spanish mackerel skin as influenced by average molecular weight10.3390/molecules190811211 ↗
9. Antioxidant, immunomodulatory and antiproliferative effects of gelatin hydrolysates from seabass (Lates calcarifer) skins10.1111/ijfs.13123 ↗
10. Supatra, T.S., Yvonne, K., Kee, M.B.O., Fitzgerald, R.J., Brien, N.M.O., and Benjakul, S. (2017). Purification and identification of antioxidant peptides from gelatin hydrolysate of seabass skin. J. Food Biochem., 1–11.
11. Optimised protein recovery from mackerel whole fish by using sequential acid/alkaline isoelectric solubilization precipitation (ISP) extraction assisted by ultrasound10.1016/j.lwt.2017.09.045 ↗
12. Al Khawli, F., Pateiro, M., Domínguez, R., Lorenzo, J.M., Gullón, P., Kousoulaki, K., Ferrer, E., Berrada, H., and Barba, F.J. (2019). Innovative green technologies of intensification for valorization of seafood and their by-products. Mar. Drugs, 17.10.3390/md17120689 ↗
13. Ivanović, M.I., Islamčević Razboršek, M., and Kolar, M. (2020). Innovative Extraction techniques using deep eutectic solvents and analytical methods for the isolation and characterization of natural bioactive compounds from plant material. Plants, 9.10.3390/plants9111428 ↗
14. Jayasinghe, G.D.T.M., Domínguez-González, R., Bermejo-Barrera, P., and Moreda-Piñeiro, A. (2020). Combining ultrasound-assisted extraction and vortex-assisted liquid–liquid microextraction for the sensitive assessment of aflatoxins in aquaculture fish species. J. Sep. Sci., 43.10.1002/jssc.201901129 ↗
15. Biological detoxification of mycotoxins: A review10.1007/s13213-014-0899-7 ↗
16. Mycotoxins: Occurrence, toxicology, and exposure assessment10.1016/j.fct.2013.07.047 ↗
17. (2006). The Commission of the European Communities Commission Regulation
18. (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Off. J. Eur. Union, L 364/5, 5-24.
19. Huang, Y., Han, D., Zhu, X., Yang, Y., Jin, J., Chen, Y., and Xie, S. (2011). Response and recovery of gibel carp from subchronic oral administration of aflatoxin B1. Aquaculture, 319.10.1016/j.aquaculture.2011.06.024 ↗
20. Nomura, H., Ogiso, M., Yamashita, M., Takaku, H., Kimura, A., Chikasou, M., Nakamura, Y., Fujii, S., Watai, M., and Yamada, H. (2011). Uptake by dietary exposure and elimination of aflatoxins in muscle and liver of rainbow trout (Oncorhynchus mykiss). J. Agric. Food Chem., 59.10.1021/jf1047354 ↗
21. Tolosa, J., Barba, F.J., Font, G., and Ferrer, E. (2019). Mycotoxin incidence in some fish products: QuEChERS methodology and liquid chromatography linear ion trap tandem mass spectrometry approach. Molecules, 24.10.3390/molecules24030527 ↗
22. Tolosa, J., Font, G., Mañes, J., and Ferrer, E. (2014). Natural occurrence of emerging Fusarium mycotoxins in feed and fish from aquaculture. J. Agric. Food Chem., 62.10.1021/jf5036838 ↗
23. An overview of conventional and emerging analytical methods for the determination of mycotoxins10.3390/ijms10010062 ↗
24. Kong, W.J., Liu, S.Y., Qiu, F., Xiao, X.H., and Yang, M.H. (2013). Simultaneous multi-mycotoxin determination in nutmeg by ultrasound-assisted solid-liquid extraction and immunoaffinity column clean-up coupled with liquid chromatography and on-line post-column photochemical derivatization-fluorescence detection. Analyst, 138.10.1039/c3an00059a ↗
25. Mycotoxins and their consequences in aquaculture: A review10.1016/j.aquaculture.2015.08.022 ↗
26. Natural occurrence of fusarium mycotoxins in aquaculture fish food
27. Munekata, P.E.S., Pateiro, M., Domínguez, R., Zhou, J., Barba, F.J., and Lorenzo, J.M. (2020). Nutritional characterization of sea bass processing by-products. Biomolecules, 10.10.3390/biom10020232 ↗
28. Martí-Quijal, F.J., Tornos, A., Príncep, A., Luz, C., Meca, G., Tedeschi, P., Ruiz, M.-J., and Barba, F.J. (2020). Impact of fermentation on the recovery of antioxidant bioactive compounds from sea bass byproducts. Antioxidants, 9.10.3390/antiox9030239 ↗
29. De la Fuente, B., López-García, G., Máñez, V., Alegría, A., Barberá, R., and Cilla, A. (2019). Evaluation of the bioaccessibility of antioxidant bioactive compounds and minerals of four genotypes of Brassicaceae microgreens. Foods, 8.10.3390/foods8070250 ↗
30. Multimycotoxin LC-MS/MS Analysis in tea beverages after dispersive liquid-liquid microextraction (DLLME)10.1021/acs.jafc.7b03507 ↗
31. (2002). European Commission Decision 2002/657/EC. Off. J. Eur. Communities, L 221/8, 8–36.
32. Recovery of tilapia (Oreochromis niloticus) protein isolate by high-intensity ultrasound-aided alkaline isoelectric solubilization/precipitation process10.1007/s11947-014-1431-6 ↗
33. Functional and nutritional characteristics of proteins and lipids recovered by isoelectric processing of fish by-products and low-value fish: A review10.1016/j.foodchem.2010.06.078 ↗
34. Franco, D., Munekata, P.E.S., Agregán, R., Bermúdez, R., López-Pedrouso, M., Pateiro, M., and Lorenzo, J.M. (2020). Application of pulsed electric fields for obtaining antioxidant extracts from fish residues. Antioxidants, 9.10.3390/antiox9020090 ↗
35. Effect of time of low-frequency ultrasound treatment on antioxidant activity and physicochemical properties of silver carp myofibrillar protein
36. Effect of mechanical separation process on lipid oxidation in European aquacultured sea bass, gilthead sea bream, and rainbow trout products10.1016/j.foodcont.2016.02.033 ↗
37. Advances in ultrasound assisted extraction of bioactive compounds from cash crops—A review10.1016/j.ultsonch.2018.07.018 ↗
38. Antioxidant and cryoprotective effects of hydrolysate from gill protein of bighead carp (Hypophthalmichthys nobilis) in preventing denaturation of frozen surimi10.1016/j.foodchem.2019.05.142 ↗
39. Kim, H.K., Ho-Kim, Y., Yun, K., Kim, Y.-J., Park, J.H., and Lee, N.H. (2012). Effects of ultrasonic treatment on collagen extraction from skins of the sea bass Lateolabrax japonicus. Fish. Sci., 78.10.1007/s12562-012-0472-x ↗
40. Characterization of protein hydrolysates from blue whiting (Micromesistius poutassou) and their application in beverage fortification10.1016/j.foodchem.2017.10.107 ↗
41. Deng, Y., Wang, Y., Deng, Q., Sun, L., Wang, R., Wang, X., Liao, J., and Gooneratne, R. (2020). Simultaneous quantification of aflatoxin B1, T-2 toxin, ochratoxin A and deoxynivalenol in dried seafood products by LC-MS/MS. Toxins, 12.10.3390/toxins12080488 ↗