Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering
Source abstract
Abstract
Inspired by the aligned extracellular matrix and bioceramics in human bone tissue, we investigated the relative contributions of nanotopography and equine bone powders (EBPs) with human dental pulp stem cells (DPSCs) to the osteogenesis. Both nanotopography and EBPs independently promoted the osteogenesis of DPSCs, osteogenesis was further promoted by the two factors in combination, indicating the importance of synergistic design factor of guided bone regeneration (GBR) membrane. The osteogenesis of DPSCs was affected by the polycaprolactone-based nanotopography of parallel nanogrooves as well as EBPs coating. Interestingly, both nanopattern and EBPs affected the DPSCs morphologies; nanopattern led to cell elongation and EBPs led to cell spreading and clustering. Analysis of the DPSCs-substrate interaction, DPSCs-EBPs interaction suggests that the combined environment of both factors play a crucial role in mediating osteogenic phenotype. This simple method to achieve a suitable environment for osteogenesis via nanotopography and EBPs coating modulation may be regarded as a promising technique for GBR/GTR membranes, which widely used dental and maxillofacial surgery applications.
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Research context
OpenAlex topics: Bone Tissue Engineering Materials · 3D Printing in Biomedical Research · Bone and Dental Protein Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Korea
Department of Rural and Biosystems Engineering, Chonnam National University, Gwangju 61186, Korea
Department of Biosystems Engineering, College of Agricultural and Life Sciences, Kangwon National University, Chuncheon 24341, Korea
Department of Industrial Mechanical Engineering, College of Life Science and Natural Resources, Sunchon National University, Sunchon 57922, Korea
Funding
National Research Foundation of Korea · Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET)
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