DataVXApplied SciencesPaper
journal article·2016-10-26Open access

The Development of Piezo-Driven Tools for Cellular Piercing

Changhai Ru, Peng Pan, Ruihua Chen

Applied Sciences· Volume 6· Issue 11
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Source abstract

Abstract

Conventionally, intracytoplasmic sperm injection (ICSI) in the mouse is conducted with piezo-drills that use a droplet of mercury for damping. The use of mercury causes concerns of toxicity and contamination. Although Fluorinert can be used as a substitute for mercury to reduce piezo-drill’s lateral vibration, the damping effect is not as satisfactory as mercury. In this work, a modified piezo-drill without using mercury was developed for the cellular piercing of mouse oocytes. Experimentally, appropriate parameters of driving voltage and frequency were obtained for the penetration of the zona pellucida of mouse oocytes. Furthermore, the lateral vibration of the injection pipette is lower than 1 μm in deionized water, which is not observable at 400 magnificence. With the piezo-drill without using mercury, the system performs the cellular piercing of mouse oocytes with a maximum cleavage rate of 94.7% (n = 117).
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Research context

OpenAlex topics: Reproductive Biology and Fertility · Microfluidic and Bio-sensing Technologies · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Jiangsu Provincial Key Laboratory of Advanced Robotics, Soochow University, Suzhou 215021, China

Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215021, China

Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China

Jiangsu Province Key Laboratory of Stem Cell Research, Soochow University, Suzhou 215006, China

Funding

Instrument Development Major Program of National Nature Science of China · Marie Curie Actions-International Research Staff Exchange Scheme “Biomedical Robotics and Applications” · Nanometer Technology Program of Science and Technology Development Plan of Suzhou

References (20)

Show source-provided references
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