journal article·2020-04-19Open access

Chemical Composition and Antimicrobial Activity of the Essential Oils of Three Closely Related Hypericum Species Growing Wild on the Island of Crete, Greece

Maria-Eleni Grafakou, Aggeliki Diamanti, Eleftheria Antaloudaki, Zacharias Kypriotakis, Ana Ćirić, Marina Soković, Helen Skaltsa

Applied Sciences· Volume 10· Issue 8

Abstract

The volatile compositions of three closely related Hypericum species growing wild on the island of Crete were studied, all belonging to the section Coridium. Hydro-distillation in a modified Clevenger-type apparatus was performed according to the Hellenic Pharmacopoeia in order to obtain the essential oils, which were analyzed by GC-MS. Identification of the compounds was carried out by comparison of MS spectra and retention indices with literature data, as well as by co-chromatography with authentic samples. In total, 123 different compounds were identified and the main compounds were by order of their abundance as follows: H. empetrifolium: α-pinene, germacrene D, β-pinene, E-caryophyllene; H. amblycalyx: β-elemene, β-selinene, α-pinene, E-caryophyllene, α-selinene; H. jovis: trans-calamenene, α-selinene, β-elemene. The chemical results revealed the differences and similarities (qualitative and quantitative) between the studied oils, supporting the hypothesis that essential oils from Hypericum spp. do not serve as chemotaxonomic markers. Moreover, the essential oils were subjected to antimicrobial screening. According to the given results, the essential oils possessed better antifungal and anticandidal activities than antibacterial activities.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Natural Compound Pharmacology Studies · Phytochemistry and Biological Activities · Plant Parasitism and Resistance

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Laboratory of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece

Division of Plant Biology, Department of Biology, University of Crete, GR-70013 Heraklion, Greece

Laboratory of Taxonomy and Management of Wild Flora, Department of Agriculture, School of Agricultural Sciences, Technological Educational Institute of Crete, Stavromenos P.O. Box 140, 71110 Crete, Greece

Institute for Biological Research ‘‘Siniša Stanković’’—National Institute of Republic of Serbia, University of Belgrade, Blvd. Despot Stefan 142, 10060 Belgrade, Serbia

Funding

State Scholarships Foundation

References (42)

Show source-provided references
1. Taxonomy and chemotaxonomy of the genus Hypericum
2. Totelin, L.M.V. (2009). Hippocratic Recipes: Oral and Written Transmission of Pharmacological Knowledge in Fifth- and Fourth-Century Greece. Studies in Ancient Medicine, Brill.10.1163/ej.9789004171541.i-366 ↗
3. Dioscorides, P. (1902). Des Pedanios Dioskurides aus Anazarbos Arzneimittellehre in Fünf Büchern: Übersetzt und Mit Erklärungen Versehen von Prof. Dr. J. Berendes, Ferdinand Enke.
4. Ethnopharmacological approach to the herbal medicines of the “Antidotes ” in Nikolaos Myrepsos’ Dynameron10.1016/j.jep.2015.01.005 ↗
5. Ethnopharmacological approach to the herbal medicines of the “Elements Alpha to Delta” in Nikolaos Myrepsos’ Dynameron. Part II10.1016/j.jep.2017.04.021 ↗
6. HMPC (2019, March 20). Community Herbal Monograph On Hypericum perforatum L., Herba (Well-Established Medicinal Use). Available online: https://www.ema.europa.eu/en/documents/herbal-monograph/final-community-herbal-monograph-hypericum-perforatum-l-herba-well-established-medicinal-use_en.pdf.
7. European Commission (2019, February 20). Opinion of the Scientific Committee on Food on the Presence of Hypericin and Extracts of Hypericum sp. in Flavourings and Other Food Ingredients with Flavouring Properties. Available online: https://ec.europa.eu/food/sites/food/files/safety/docs/fs_food-improvement-agents_flavourings-out113.pdf.
8. A Survey on the Hypericum Genus: Secondary Metabolites and Bioactivity10.1016/S1572-5995(05)80043-2 ↗
9. Essential Oil and volatile components of the genus Hypericum (Hypericaceae)
10. Hypericum species as sources of valuable essential oils
11. Hypericum sp.: Essential oil composition and biological activities10.1007/s11101-012-9223-y ↗
12. Chemical composition and larvicidal activity of several essential oils from Hypericum Species from Tunisia10.1007/s00436-012-3189-y ↗
13. Rouis, Z., Abid, N., Koudja, S., Yangui, T., Elaissi, A., Cioni, P.L., and Aouni, M. (2013). Evaluation of the cytotoxic effect and antibacterial, antifungal, and antiviral activities of Hypericum triquetrifolium Turra essential oils from Tunisia. BMC Complement. Altern. Med., 13.10.1186/1472-6882-13-24 ↗
14. Repellency of three plant essential oils against Red Flour Beetle Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae)
15. Studies in the Genus Hypericum L (Hypericaceae) 5 (2). Sections 17. Hirtella to 1910.11646/phytotaxa.4.1.3 ↗
16. Scientific Committee of the Hellenic Pharmacopoeia (2002). Hellenic Pharmacopoeia.
17. A generalization of the retention index system including linear temperature programmed gas—Liquid partition chromatography10.1016/S0021-9673(01)80947-X ↗
18. Adams, R.P.M. (2017). Identification Of Essential Oil Components By Gas Chromatography/Mass Spectrometry, Allured Publishing. [4.1 ed.].
19. A comparison of the E-test with the NCCLS M38-P method for antifungal susceptibility testing of common and emerging pathogenic filamentous fungi10.1128/JCM.39.4.1360-1367.2001 ↗
20. Clinical and Laboratory Standards Institute (2009). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Clinical and Laboratory Standards Institute. [8th ed.]. CLSI publication M07-A8.
21. Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drugre sistant bacteria10.1016/j.mimet.2012.05.001 ↗
22. Essential Oil Composition of Hypericum perfoliatum L. Growing in Different Locations in Greece10.1002/ffj.979 ↗
23. Composition and antimicrobial activity of the essential oil of Hypericum rumeliacum subsp. apollinis (Boiss. & Heldr.)10.1002/ptr.1093 ↗
24. A method for detecting the biosystematic significance of the essential oil composition: The Case of Five Hellenic Hypericum L. Species10.1016/j.bse.2005.02.002 ↗
25. The Essential Oil of Hypericum Perforatum L. Hypericum tetrapterum Fries and Hypericum olympicum L. growing in Greece10.1002/ffj.1521 ↗
26. Hippocratic Medicinal Flora on the Greek Island of Kos: Spatial distribution, assessment of soil conditions, essential oil content and chemotype analysis
27. Étude chimio-taxonomique du genre Hypericum: II. Identification de constituants de diverses huiles essentielles d’Hypericum10.1016/S0031-9422(00)84003-0 ↗
28. Étude chimio-taxonomique du genre Hypericum: III. Répartition des carbures saturés et des monoterpènes dans les huiles essentielles d’Hypericum10.1016/S0031-9422(00)84004-2 ↗
29. Chemotaxonomy of Hypericum genus from Portugal: Geographical distribution and essential oils composition of Hypericum perfoliatum, Hypericum humifusum, Hypericum linarifolium and Hypericum pulchrum10.1016/j.bse.2007.07.004 ↗
30. Essential oil composition of Hypericum L. species from southeastern serbia and their chemotaxonomy10.1016/j.bse.2006.09.012 ↗
31. Antibacterial and cytotoxic activity of prenylated bicyclic acylphloroglucinol derivatives from Hypericum amblycalyx10.1515/znc-2003-7-814 ↗
32. Hyperjovinols A and B: Two New Phloroglucinol Derivatives from Hypericum jovis with antioxidant activity in cell cultures10.1021/np034051w ↗
33. Strid, A. (2016). Atlas of the Aegean Flora, Botanic Garden and Botanical Museum Berlin, Freie Universität Berlin.
34. Essential Oil composition of six Hypericum species from Southern Brazil10.1002/ffj.1435 ↗
35. Composition and Antimicrobial Activity of the Essential Oil of Hypericum coris10.1016/S0367-326X(02)00171-5 ↗
36. Waxes and Volatile Oils in Hypericum ericoides (Guttiferae)10.1007/BF02535432 ↗
37. Volatile Constituents of two Hypericum species from Tunisia
38. Volatile Components of Hypericum humifusum, Hypericum perforatum and Hypericum ericoides by HS-SPME-GC and HS-SPME-GCMS Using Nano Scale Injection Techniques
39. Mycotoxin review—1. Aspergillus and Penicillium
40. Ochratoxin A Producing Species in the Genus Penicillium10.3390/toxins2051111 ↗
41. The pathobiology of Aspergillus fumigatus10.1016/S0966-842X(01)02104-7 ↗
42. Potential of Cameroonian plants and derived products against microbial infections: A review10.1055/s-0030-1250027 ↗