Functional Evaluation of Fucus vesiculosus Extract: Bioactivity Retention After In Vitro Digestion and Anti-Inflammatory Effects on Murine Peritoneal Macrophages
Source abstract
Abstract
Background: Nowadays, to improve animal production sustainably, the zootechnical sector is exploring novel, functional ingredients, such as seaweed. This study investigated the functional properties of Fucus vesiculosus and their persistence after simulated digestion. Methods: F. vesiculosus was nutritionally characterized (AOAC methods) and digested in vitro through the INFOGEST protocol. The polyphenol, flavonoid, and phlorotannin contents of the samples were analyzed through colorimetric assays. The antioxidant properties were evaluated using ABTS assay and the growth inhibition capacity against Escherichia coli using the microdilution method. The cytotoxic activity and anti-inflammatory properties were evaluated on mouse peritoneal macrophages using crystal violet assay and the gene expression of IL-1β, IL-6, TNF-α, and iNOS. Results: F. vesiculosus demonstrated high levels of dietary fiber (47.36%) and protein (13.99%). Significant levels of polyphenols (6428.98 µg TAE/g), flavonoids (5171.31 µg CE/g), and phlorotannins (2.10 mg PGE/g) were detected. These bioactive compounds allowed for strong antioxidant activity (85.96% ABTS+ scavenging) and E. coli growth inhibition (17%). Simulated digestion minimally impacted the content of bioactive compounds and their associated functional properties. F. vesiculosus exhibited a protective effect against oxidative stress in macrophages, downregulating pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α). Conclusions: These findings support the potential of F. vesiculosus as a functional feed ingredient for livestock, maintaining its beneficial properties even after digestion.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Seaweed-derived Bioactive Compounds · Microbial Metabolites in Food Biotechnology · Proteins in Food Systems
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Veterinary Medicine and Animal Sciences—DIVAS, University of Milan, Via dell’Università 6, 26900 Lodi, Italy
Animal Care Sector—Research Services Division, University of Milan, Via dell’Università 6, 26900 Lodi, Italy
Biotecnologie BT, Frazione Pantalla, 06059 Todi, Italy
Department of Agricultural and Environmental Sciences-Production Landscape and Agroenergy, University of Milano, Via Celoria 2, 20133 Milan, Italy
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