Identification of Previously Unrecorded Bacills, Serratia, and Mucor Strains Isolated from Yogurt
Source abstract
Abstract
(1) Background: Foodborne illnesses are recognized as a significant threat to public health and the economy in both developed and developing nations. The safety of foods containing microorganisms has consequently become a major worldwide concern. One of the most frequent causes of food deterioration in the world is microbial contamination. (2) Methods: Yogurt containers that were bought commercially in Riyadh, Saudi Arabia during their validity period contained four different species of bacteria and one type of fungus. Using nutritional agar and Czapek-Dox agar medium, the bacteria and fungi were isolated. The isolates of the fungi and bacteria were identified using a scanning microscope. The isolates were further identified and classified for molecular evolutionary analyses using the 16S rRNA and ITS sequences from the bacteria and fungi, respectively, in conjunction with the universal primers 27F, 1492R, ITS1F, and ITS4R. (3) Results: A total of 131 separate strains were identified from 12 yogurt samples based on their phenotypic characteristics. In total, 79 isolates (60.3%) consisted of Serratia marcescens, Bacillus subtilis, and Mucor circinelloides, with 52 isolates (39.7%) being Bacillus cereus. While the cells of Bacillus and Serratia are shaped like rods, the sporangia of Mucor are large, round, and black. Each strain was identified by its accession number, which were as follows: MK590996.1: B. cereus; MK591144.1: B. subtilis; MK591002.1 and MK591014.1: S. marcescens; and MK559692.1: M. circinelloides. The maximum identification was found to be between 98.64 and 100% when BLAST was used to compare the sequences to the NCBI GeneBank database. (4) Conclusions: Genus and species identification was performed using the similarity score values. Yogurt products containing high concentrations (39.7%) of Bacillus cereus isolates carry a significant risk of health hazards due to the potential for spreading pathogenic bacteria to humans.
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Research context
OpenAlex topics: Probiotics and Fermented Foods · Identification and Quantification in Food · Enzyme Production and Characterization
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biology, College of Sciences, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
References (58)
Show source-provided references
1. Spoilage of Dairy Products Due to Fungi
2. Toxinogenic and Spoilage Potential of Aerobic Spore-Formers Isolated from Raw Milk10.1016/j.ijfoodmicro.2009.11.007 ↗
3. Microbiological Quality of Soft, Semi-Hard and Hard Cheeses during the Shelf-Life10.1515/macvetrev-2015-0068 ↗
4. Evaluation of Microbial Load and Quality of Milk & Milk Based Dairy Products
5. The Complex Microbiota of Raw Milk10.1111/1574-6976.12030 ↗
6. The Members of the Bacillus Cereus Group Are Commonly Present Contaminants of Fresh and Heat-Treated Milk10.1016/j.fm.2008.02.001 ↗
7. A Risk Assessment Study ofBacillus Cereuspresent in Pasteurized Milk10.1006/fmic.1996.0076 ↗
8. Occurrence of Emetic Toxin Producing Bacillus Cereus in the Dairy Production Chain10.1016/j.idairyj.2005.07.002 ↗
9. Occurrence and Behavior of Bacillus Cereus in Naturally Contaminated Ricotta Salata Cheese during Refrigerated Storage10.1016/j.fm.2016.05.002 ↗
10. Muehlhoff, E., and Bennett, A. (2013). Milk and Dairy Products in Human Nutrition, Food and Agriculture Organization of the United Nations.
11. Tamime, A.Y., Tamine, A.Y., Robinson, R.K., and Robinson, R.K. (2007). Tamime and Robinson’s Yoghurt Science and Technology, Taylor & Francis. [3rd ed.].10.1533/9781845692612 ↗
12. Microbiological Quality of Yoghurt Commercialized in Viçosa, Minas Gerais, Brazil
13. Hygienic and Microbial Quality of Yoghurt
14. Tracking Spore-Forming Bacteria in Food: From Natural Biodiversity to Selection by Processes10.1016/j.ijfoodmicro.2012.03.004 ↗
15. Vos, P., Garrity, G., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K.-H., and Whitman, W.B. (2011). Bergey’s Manual of Systematic Bacteriology: Volume 3: The Firmicutes, Springer Science & Business Media.
16. Detection of Toxigenic Strains of Bacillus Cereus and Other Bacillus Spp. with an Improved Cytotoxicity Assay10.1046/j.1365-2672.1999.00498.x ↗
17. Toxin-Producing Ability among Bacillus Spp. Outside the Bacillus Cereus Group10.1128/AEM.71.3.1178-1183.2005 ↗
18. Studies on Enterotoxigenic Bacillus Cereus in Raw Milk and Some Dairy Products10.1111/j.1745-4565.2010.00226.x ↗
19. Prevalence and Characterization of Bacillus Cereus Group from Various Marketed Dairy Products in India10.1007/s13594-014-0174-5 ↗
20. Serratia Infections: From Military Experiments to Current Practice10.1128/CMR.00017-11 ↗
21. Cleto, S., Matos, S., Kluskens, L., and Vieira, M.J. (2012). Characterization of Contaminants from a Sanitized Milk Processing Plant. PLoS ONE, 7.10.1371/journal.pone.0040189 ↗
22. Characterization of Gram-Negative Psychrotrophic Bacteria Isolated from Italian Bulk Tank Milk10.1111/1750-3841.12645 ↗
23. Screening of Bacteria Responsible for the Spoilage of Milk
24. Garnier, L., Valence, F., and Mounier, J. (2017). Diversity and Control of Spoilage Fungi in Dairy Products: An Update. Microorganisms, 5.10.3390/microorganisms5030042 ↗
25. Microbial and Biochemical Spoilage of Foods: An Overview10.1016/0168-1605(96)01139-7 ↗
26. Pitt, J.I., and Hocking, A.D. (2009). Fungi and Food Spoilage, Springer.10.1007/978-0-387-92207-2 ↗
27. Epidemiology and Clinical Manifestations of Mucormycosis10.1093/cid/cir866 ↗
28. Zygomycosis in Solid Organ Transplant Recipients: A Prospective, Matched Case-Control Study to Assess Risks for Disease and Outcome10.1086/605445 ↗
29. Characterization and Control of Mucor Circinelloides Spoilage in Yogurt10.1016/j.ijfoodmicro.2016.04.008 ↗
30. Clark, S., Jung, S., and Lamsal, B. (2014). Food Processing: Principles and Applications, John Wiley & Sons.10.1002/9781118846315 ↗
31. MacBean, R.D. (2010). Food Packaging and Shelf Life, Taylor and Francis Group, LLC.10.1201/9781420078459-c8 ↗
32. A Method for Counting the Number of Fungi in the Soil10.1128/jb.7.3.339-341.1922 ↗
33. Microbial Diversity in Water and Sediment of Lake Chaka, an Athalassohaline Lake in Northwestern China10.1128/AEM.02869-05 ↗
34. Geneious Basic: An Integrated and Extendable Desktop Software Platform for the Organization and Analysis of Sequence Data10.1093/bioinformatics/bts199 ↗
35. Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol. Biol. Evol., 35.10.1093/molbev/msy096 ↗
36. MEGA11: Molecular Evolutionary Genetics Analysis Version 1110.1093/molbev/msab120 ↗
37. PAST: Paleontological Statistics Software Package for Education and Data Analysis
38. Microbial Assessment of Some Commercially Prepared Yoghurt Retailed in Minna, Niger State
39. Asafo-Agyei, K.O., and Samant, H. (2023). Hepatocellular Carcinoma, StatPearls Publishing.
40. Microbiological Assessment of Commercial Yogurt Sold in Ota Metropolis, Ogun State, Nigeria10.1088/1755-1315/210/1/012019 ↗
41. Isolation and Genotypic Identification of Some Spoilage and Pathogenic Microbes from Yogurt10.21608/bvmj.2022.147816.1539 ↗
42. Prevalence of Enterobacteriaceae in Raw Milk and Some Dairy Products10.21608/kvmj.2020.39992.1009 ↗
43. Purification and Characterization of a Phytoalexin Elicitor from Spores of the Saprobe Mucor Ramosissimus10.1590/S0100-84042005000400008 ↗
44. A Biotechnology Perspective of Fungal Proteases10.1590/S1517-838246220140359 ↗
45. Isolation and Characterization of Four Unrecorded Mucor Species in Korea10.1080/12298093.2019.1703373 ↗
46. Mycological Evaluation of Milk and Some Milk Products in Beni-Suef City10.21608/avmj.2018.168711 ↗
47. Evaluation of Fungi Species from Commercial Yoghurt in Birnin Kebbi, Kebbi State Nigeria
48. Fungal Contamination of Some Local Dairy Products and Extent Production of Aflatoxins
49. Reducing Food Losses by Intelligent Food Logistics10.1098/rsta.2013.0302 ↗
50. The Shelf Life of Yogurt Starter and Its Derivatives Based on the Microbiological, Physical and Sensory Aspects10.1088/1755-1315/462/1/012014 ↗
51. Survival of Bifidobacteria during Refrigerated Storage in the Presence of Acid and Hydrogen Peroxide
52. Nuclear Ribosomal Internal Transcribed Spacer (ITS) Region as a Universal DNA Barcode Marker for Fungi10.1073/pnas.1117018109 ↗
53. Dentinger, B.T., Didukh, M.Y., and Moncalvo, J.-M. (2011). Comparing COI and ITS as DNA Barcode Markers for Mushrooms and Allies (Agaricomycotina). PLoS ONE, 6.10.1371/journal.pone.0025081 ↗
54. DNA Barcoding of Lichenized Fungi Demonstrates High Identification Success in a Floristic Context10.1111/j.1469-8137.2011.03677.x ↗
55. DNA Barcoding of Fungi: A Case Study Using ITS Sequences for Identifying Aquatic Hyphomycete Species10.1007/s13225-010-0056-y ↗
56. Diaporthe: A Genus of Endophytic, Saprobic and Plant Pathogenic Fungi10.3767/003158513X666844 ↗
57. Highly Efficient Concerted Evolution in the Ribosomal DNA Repeats: Total rDNA Repeat Variation Revealed by Whole-Genome Shotgun Sequence Data10.1101/gr.5457707 ↗
58. Monitoring Microbial Quality of Commercial Dairy Products in West Azerbaijan Province, Northwest of Iran10.1016/S2222-1808(14)60735-0 ↗