Optimization of Impedance-Based Real-Time Assay in xCELLigence RTCA SP16 Device for the Analysis of Fully Differentiated Caco-2 Cells
Source abstract
Abstract
Impedance-based cellular assays allow determination of biological functions of cell populations in real-time by measuring electrical impedance. As compared to end-point assays, such as trans-epithelial electrical resistance assays, for example, they enable fast, non-invasive, and easy detection of cell kinetics—their growth, attachment, and interaction can be monitored over time. In our experiment, Caco-2 cells were cultured on E-plates 16. Next, fully differentiated cells were treated with either TNF-α or 3,4-dihydroxy-L-phenylalanine (L-DOPA). We aimed to verify the possibility of real-time testing of the viability, monolayer formation, and integrity (i.e., the presence of a functional and polarized monolayer) of Caco-2 cells by the xCELLigence real-time cell analyzer (RTCA) S16 system (Agilent Technologies).
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Research context
OpenAlex topics: 3D Printing in Biomedical Research · Microfluidic and Bio-sensing Technologies · Cell Image Analysis Techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Doctoral School of Medical and Health Sciences, Jagiellonian University Medical College, św. Łazarza 16, 31-530 Cracow, Poland
Department of Pathophysiology, Faculty of Medicine, Jagiellonian University Medical College, Czysta 18, 31-121 Cracow, Poland
Center for the Development of Therapies for Civilization and Age-Related Diseases, Jagiellonian University Medical College, Medyczna 9, 31-066 Cracow, Poland
Faculty of Pharmacy, Department of Technology and Biotechnology of Drugs, Jagiellonian University Medical College, Medyczna 9, 31-008 Cracow, Poland
Funding
Priority Research Area qLife under the program “Excellence Initiative—Research University” at the Jagiellonian University in Krakow · Jagiellonian University Medical College
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