Preliminary Insights into 3D Cheiloscopy for Forensic Applications: A Pilot Study

Background: Cheiloscopy, a forensic technique based on the uniqueness of labial traces, has traditionally relied on analog methods. While simple, these methods present significant limitations in terms of precision and reproducibility. The introduction of 3D technology, specifically high-resolution optical scanners, represents a pivotal advancement in overcoming these challenges. Objective: This pilot study aimed to explore the feasibility and potential advantages of 3D cheiloscopy in forensic science by analyzing its precision and repeatability compared to traditional analog methods. Methods: Two participants were selected as a pilot sample, and their lip impressions were captured using both analog techniques and a high-resolution intraoral scanner (Medit i700). A total of 40 samples were collected, equally distributed among operators with varying levels of experience. Surface deviation analysis was performed to compare the methods. Results: The 3D models demonstrated greater uniformity and resolution compared to the analog impressions, which were more prone to errors caused by material quality or operator pressure. Surface deviation analysis showed an average similarity of 70%, with a standard deviation of 0.229 mm. The digital methods also significantly reduced operator-dependent variability. Conclusions: This pilot study highlights the potential of 3D cheiloscopy to improve precision and standardization in forensic applications. While the small sample size limits the generalizability of the findings, the results provide a foundation for further research with larger and more diverse datasets. Future studies should explore the capability of 3D cheiloscopy to accurately match individuals and further validate its applicability in forensic and clinical contexts.