Molecular Insights into the Interactions Between Human Serum Albumin and Phospholipid Membranes
Source abstract
Abstract
In this study, molecular dynamics simulations were employed to analyze interactions between phospholipid membranes and human serum albumin (HSA) in the presence of mono- and divalent cations. Two types of membranes, composed of dipalmitoyl phosphatidylcholine (DPPC) and dipalmitoyl phosphatidylethanolamine (DPPE), were utilized. The results revealed that both systems exhibited high stability. The DPPE complexes displayed a greater affinity for albumin compared to DPPC. The high stability of the complexes was attributed to a high number of ionic contacts and hydrogen bonds. The presence of mono- and divalent metal cations significantly influenced the membrane’s capacity to bind proteins. However, these effects varied depending on the phospholipid composition of the bilayer. The studies confirmed the relatively low ability of DPPC to bind potassium ions, as previously observed by others. Consequently, the DPPC/HSA/K+ complex was found to be the least stable among the systems studied. While DPPC interactions were limited to HSA domains I and II, DPPE was able to interact with all domains of the protein. Both lipid bilayers exhibited substantial structural changes and characteristic curvature induced by interactions with HSA, which confirms the formation of relatively strong interactions capable of influencing the arrangement of the phospholipids.
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Research context
OpenAlex topics: Protein Interaction Studies and Fluorescence Analysis · Lipid Membrane Structure and Behavior · Surfactants and Colloidal Systems
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Physical Chemistry, Pharmacy Faculty, Collegium Medicum of Bydgoszcz, Nicolaus Copernicus University in Toruń, Kurpińskiego 5 Street, PL-85096 Bydgoszcz, Poland
Institute of Computer Science, Kazimierz Wielki University in Bydgoszcz, Kopernika 1, PL-85074 Bydgoszcz, Poland
Faculty of Telecommunications, Computer Science and Electrical Engineering, Bydgoszcz University of Science and Technology, Kaliskiego 7 Street, PL-85796 Bydgoszcz, Poland
Faculty of Mechanical Engineering, Bydgoszcz University of Science and Technology, Kaliskiego 7 Street, PL-85796 Bydgoszcz, Poland
Faculty of Science and Technology, University of Silesia in Katowice, 75 Pułku Piechoty 1A, PL-41500 Chorzów, Poland
School of Engineering Sciences in Chemistry, Biotechnology and Health, Engineering Pedagogics, KTH Royal Institute of Technology, SE-10044 Stockholm, Sweden
Division of Surface and Corrosion Science, Department of Chemistry, KTH Royal Institute of Technology, SE-10044 Stockholm, Sweden
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