journal article·2025-02-01Open access

Application of the Allium Test in Toxicity Studies of Lead and Copper: A Cytological Perspective

Emilian Onisan, Ioan Sarac, Cerasela Petolescu, Marinel Nicolae Horablaga, Cristian Mate, Alina Simina, Dorin Camen, Mariana Ganea, Daniela Rebeca Ardelean, Lucian Cãlugar, Irina Petrescu, Ramona Ștef

Applied Sciences· Volume 15· Issue 3

Abstract

The Allium test is a cytological method used to monitor the impact of heavy metals. It can be used to evaluate meristematic tissues and highlight abnormalities occurring during mitotic division, with the advantage of being both rapid and economical. Copper and lead are among the most widespread metals in everyday life, mainly due to the worldwide expansion of industrialization, and are present in soil, water, and air. Using Allium sativum as a bioindicator for this study, statistical analysis confirmed significant differences in genotoxicity between the two metals, reflected by the inhibition of mitotic activity (MI) or increased indices of cellular abnormalities (AI). Toxicity was dose- and time-dependent for both metals, with copper exhibiting greater genotoxic effects than lead. Copper caused a significant reduction in MI, even at relatively low concentrations, with the IC50 observed at 0.50 mM after 72 h of exposure. In contrast, for lead, the IC50 was recorded from 0.75 mM after 72 h exposure. The advantages of the Allium test were demonstrated by its simplicity and high sensitivity in detecting abnormalities. In our experiment, chromosome abnormalities such as chromosome bridges, as well as isolated, delayed, or sticky chromosomes, were observed. In addition, at a concentration of 0.25 mM for copper (72 h exposure) and 0.50 mM for lead (72 h exposure), cellular abnormalities, including giant cells and binucleated cells, were identified.
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Research context

OpenAlex topics: Heavy Metal Exposure and Toxicity · Aluminum toxicity and tolerance in plants and animals · Carcinogens and Genotoxicity Assessment

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Horticulture, Faculty of Engineering and Applied Technologies, University of Life Sciences “King Mihai I” from Timisoara, 119 Calea Aradului Street, 300645 Timisoara, Romania

Faculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, Romania

Faculty of Agriculture, University of Life Sciences “King Mihai I” from Timisoara, 300645 Timisoara, Romania

Funding

“Increasing the impact of excellence research on innovation and technology transfer capacity at USV Timisoara”

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