Combinations of Echinacea (Echinacea purpurea) and Rue (Ruta gravolens) Plant Extracts with Lytic Phages: A Study on Interactions
Source abstract
Abstract
The use of combined biocontrol strategies to combat bacterial-related issues is an increasingly popular approach. Therefore, a novel investigation was performed, where interactions of lytic bacteriophages (MS2, T4 and phi6) and methanolic plant extracts (Echinacea purpurea (EP) and Ruta graveolens (RG)) in the bacterial environment have been examined to understand their application potential and limitations. Due to the complexity of these interactions, many up-to-date techniques were used (microdilution method, phage extract coincubation assay, static interactions synographies and dynamic growth profile experiments in a bioreactor). As a result of our study, antagonism interactions were observed: EP and RG extracts showed antiphage and bacterial stimulating activity. Effects caused by low extract concentrations on microorganisms depended on the species of phage and bacteria, while high concentrations suppressed bacterial lysis in general. Moreover, interactions observed in the static environment differed from those performed in a dynamic environment, showing the importance of performing multiple analyses when investigating such complex mixtures.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Herbal Medicine Research Studies · Transgenic Plants and Applications · Toxin Mechanisms and Immunotoxins
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology in Szczecin, Piastów Avenue 45, 70-311 Szczecin, Poland
Center of Bioimmobilisation and Innovative Packaging Materials, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology in Szczecin, Janickiego 35, 71-270 Szczecin, Poland
References (47)
Show source-provided references
1. Silver nanoparticle production by Ruta graveolens and testing its safety, bioactivity, immune modulation, anticancer, and insecticidal potentials
2. Coelho, J., Barros, L., Dias, M.I., Finimundy, T.C., Amaral, J.S., Alves, M.J., Calhelha, R.C., Santos, P.F., and Ferreira, I.C.F.R. (2020). Echinacea purpurea (L.) Moench: Chemical characterization and bioactivity of its extracts and fractions. Pharmaceuticals, 13.10.3390/ph13060125 ↗
3. Chemical composition, antibacterial and antifungal activities of Ruta graveolens L. volatile oils10.1080/23312009.2016.1220055 ↗
4. Chemical composition and FTIR of ethane extracts of Larrea tridentata, Origanum vulgare, Artemisa ludoviciana and Ruta graveolens
5. Echinacea purpurea supplementation stimulates select groups of human gastrointestinal tract microbiota10.1111/j.1365-2710.2006.00781.x ↗
6. Echinacea extracts contain significant and selective activities against human pathogenic bacteria10.1080/13880200701734919 ↗
7. In vitro activity of a combination of bacteriophages and antimicrobial plant extracts10.1111/lam.12838 ↗
8. Editorial: Phage therapy: Past, present and future10.3389/fmicb.2017.00981 ↗
9. Bacteria and bacteriophage inactivation by silver and zinc oxide nanoparticles10.1016/j.colsurfb.2011.02.023 ↗
10. Stachurska, X., Cendrowski, K., Pachnowska, K., Piegat, A., Mijowska, E., and Nawrotek, P. (2022). Nanoparticles influence lytic phage T4-like performance in vitro. Int. J. Mol. Sci., 23.10.3390/ijms23137179 ↗
11. Antiphage activity in extracts of plants growing in Greece10.1016/S0944-7113(97)80055-4 ↗
12. Antiphage activity of natural and synthetic substances: A new age for antivirals?10.2217/fmb-2019-0320 ↗
13. Survival of the enveloped bacteriophage Phi6 (a surrogate for SARS-CoV-2) in evaporated saliva microdroplets deposited on glass surfaces10.1038/s41598-020-79625-z ↗
14. Persistence of bacteriophage Phi6 on porous and nonporous surfaces and the potential for its use as an Ebola virus or coronavirus surrogate10.1128/AEM.01482-20 ↗
15. Stachurska, X., Roszak, M., Jabłońska, J., Mizielińska, M., and Nawrotek, P. (2021). Double-layer agar (DLA) modifications for the first step of the phage-antibiotic synergy (PAS) identification. Antibiotics, 10.10.3390/antibiotics10111306 ↗
16. Enumeration of bacteriophages by double agar overlayplaque assay10.1007/978-1-60327-164-6_7 ↗
17. Salachna, P., Grzeszczuk, M., Meller, E., and Mizielińska, M. (2019). Effects of gellan oligosaccharide and NaCl stress on growth, photosynthetic pigments, mineral composition, antioxidant capacity and antimicrobial activity in red perilla. Molecules, 24.10.3390/molecules24213925 ↗
18. Ordon, M., Nawrotek, P., Stachurska, X., Schmidt, A., and Mizielińska, M. (2021). Mixtures of Scutellaria baicalensis and Glycyrrhiza L. extracts as antibacterial and antiviral agents in active coatings. Coatings, 11.10.3390/coatings11121438 ↗
19. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances10.1038/nprot.2007.521 ↗
20. Phage-antibiotic synergy is driven by a unique combination of antibacterial mechanism of action and stoichiometry10.1128/mBio.01462-20 ↗
21. Echinacea purpurea: Pharmacology, phytochemistry and analysis methods10.4103/0973-7847.156353 ↗
22. Echinacea species (Echinacea angustifolia (DC.) Hell., Echinacea pallida (Nutt.) Nutt., Echinacea purpurea (L.) Moench): A review of their chemistry, pharmacology and clinical properties10.1211/0022357056127 ↗
23. Phytochemistry and pharmacological properties of Ruta graveolens L.
24. Ruta graveolens: From traditional system of medicine to modern pharmacology: An overview
25. Efficiency of Echinacea purpurea on performance and immune status in pigs10.1111/j.1439-0396.2005.00501.x ↗
26. Efficiency of using Echinacea purpurea in feeding laying hens of a parent flock
27. Dietary supplementation effects with Ruta graveolens on performance, carcass traits and meat quality on rabbits
28. Prophylactic delivery of a bacteriophage cocktail in feed significantly reduces Salmonella colonization in pigs10.1128/spectrum.00422-22 ↗
29. Echinacea plants as antioxidant and antibacterial agents: From traditional medicine to biotechnological applications10.1002/ptr.6101 ↗
30. Antioxidant and antimicrobial activities of Echinacea (Echinacea purpurea L.) extracts obtained by classical and ultrasound extraction10.1016/S1004-9541(08)60234-7 ↗
31. Studies on phytochemical, antioxidant, anti-inflammatory, hypoglycaemic and antiproliferative activities of Echinacea purpurea and Echinacea angustifolia extracts10.1080/13880209.2016.1265989 ↗
32. High-potency anti-influenza therapy by a combination of Echinacea purpurea fresh herb and root tinctures
33. Applications of the phytomedicine Echinacea purpurea (Purple Coneflower) in infectious diseases10.1155/2012/769896 ↗
34. Anti-viral properties and mode of action of standardized Echinacea purpurea extract against highly pathogenic avian influenza virus (H5N1, H7N7) and swine-origin H1N1 (S-OIV)10.1186/1743-422X-6-197 ↗
35. Phytochemical screening and evaluation of antibacterial activity of Ruta graveolens L.-a medicinal plant grown around Mekelle, Tigray, Ethiopia10.4172/2329-6836.1000195 ↗
36. Antifungal and antibacterial effects of Ruta graveolens extracts
37. Phytochemical investigation, GC-MS profile and antimicrobial activity of a medicinal plant Ruta graveolens L. from Ethiopia10.22159/ijpps.2017v9i6.16812 ↗
38. Antimicrobial and cytotoxic activity of Ruta graveolens10.1016/j.fitote.2005.02.008 ↗
39. Study on antibacterial effect of Ruta graveolens extracts on pathogenic bacteria
40. Natural fungicides from Ruta graveolens L. leaves, including a new quinolone alkaloid10.1021/jf0259361 ↗
41. Antiviral effects of Aloe vera (L.) Burm. f. and Ruta graveolens L. extract on acyclovir-resistant herpes simplex virus type 1
42. Effect of root extract from Boerhaavia diffusa L., containing an antiviral principle upon plaque formation of RNA bacteriophages10.1016/S0232-4393(86)80019-1 ↗
43. Antiviral effects of persimmon extract on human norovirus and its surrogate, bacteriophage MS210.1111/1750-3841.12462 ↗
44. A modified MS2 bacteriophage plaque reduction assay for the rapid screening of antiviral plant extracts10.4103/0974-8490.69108 ↗
45. In vitro evaluation of the antiviral activity of an extract of date palm (Phoenix dactylifera L.) pits on a Pseudomonas phage
46. Antiviral activity of borage (Echium amoenum)10.5114/aoms.2010.14256 ↗
47. Investigation of different interactions between Staphylococcus aureus phages and pomegranate peel, grape seed, and black cumin extracts10.1111/jfs.12679 ↗