Versatile Mass Spectrometry-Based Intraoperative Diagnosis of Liver Tumor in a Multiethnic Cohort
Source abstract
Abstract
Currently used techniques for intraoperative assessment of tumor resection margins are time-consuming and laborious and, more importantly, lack specificity. Moreover, pathological diagnosis during surgery does not often give a clear outcome. Recent advances in mass spectrometry (MS) and instrumentation have made it possible to obtain detailed molecular information from tissue specimens in real-time, with minimal sample pre-treatment. Probe Electro Spray Ionization MS (PESI-MS), combined with artificial intelligence (AI), has demonstrated its effectiveness in distinguishing liver cancer tissues from healthy tissues in a large Italian population group. As the MS profile can reflect the patient’s ethnicity, dietary habits, or particular operating room procedures, the AI algorithm must be well trained to distinguish different groups. We used a large dataset composed of liver tumor and healthy specimens, from the Italian and Japanese populations, to develop a versatile algorithm free from ethnic bias. The system can classify tissues with discrepancies <5% from the pathologist’s diagnosis. These results demonstrate the potential of the PESI-MS system to distinguish tumor from surrounding non-tumor tissues in patients, with minimal bias from race/ethnicity or etiological characteristics or operating room procedures.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Mass Spectrometry Techniques and Applications · Advanced Proteomics Techniques and Applications · Laser-induced spectroscopy and plasma
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Shimadzu Italia S.r.l., Via G.B. Cassinis, 7, 20139 Milano, Italy
Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Via Mario Negri 2, 20156 Milan, Italy
Department of Biomedical Science, Humanitas University, Via Rita Levi Montalcini 4, 20090 Pieve Emanuele, Italy
Department of Hepatobiliary and General Surgery, IRCCS Humanitas Research Hospital, Via Manzoni 56, 20089 Rozzano, Italy
Laboratory of Hepatobiliary Immunopathology, IRCCS Humanitas Research Hospital, Via Manzoni 56, 20089 Rozzano, Italy
Internal Medicine and Hepatology Unit, Department of Gastroenterology, Humanitas Clinical and Research Hospital—IRCCS, Via Manzoni 56, 20089 Rozzano, Italy
Department of Pathology, Humanitas University, Humanitas Clinical and Research Hospital—IRCCS, Via Manzoni 56, 20089 Rozzano, Italy
Shimadzu Corporation, Kyoto 604-8511, Japan
Department of Anatomy, Teikyo University School of Medicine, 2-11-1, Kaga, Itabashi, Tokyo 173-8605, Japan
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Yamanashi, 1110, Chuo, Yamanashi 409-3898, Japan
References (33)
Show source-provided references
1. Safe Hepatectomy Selection Criteria for Hepatocellular Carcinoma Patients: A Validation of 336 Consecutive Hepatectomies. The BILCHE Score10.1007/s00268-014-2786-6 ↗
2. A Gas-Liquid-Chromatographic Procedure for Separating a Wide Range of Metabolites Occurring in Urine or Tissue Extracts10.1042/bj1010792 ↗
3. Mass Spectrometry Applied to Clinical Practice and Research10.1136/bmj.1.5029.1205 ↗
4. Mass Spectrometry Technologies to Advance Care for Cancer Patients in Clinical and Intraoperative Use10.1002/mas.21664 ↗
5. Intraoperative Tissue Identification Using Rapid Evaporative Ionization Mass Spectrometry10.1126/scitranslmed.3005623 ↗
6. Eberlin, L.S., Margulis, K., Planell-Mendez, I., Zare, R.N., Tibshirani, R., Longacre, T.A., Jalali, M., Norton, J.A., and Poultsides, G.A. (2016). Pancreatic Cancer Surgical Resection Margins: Molecular Assessment by Mass Spectrometry Imaging. PLoS Med., 13.10.1371/journal.pmed.1002108 ↗
7. Mass Spectrometry Imaging as a Tool for Surgical Decision-making10.1002/jms.3295 ↗
8. Intraoperative Mass Spectrometry Mapping of an Onco-Metabolite to Guide Brain Tumor Surgery10.1073/pnas.1404724111 ↗
9. Intraoperative Assessment of Tumor Margins during Glioma Resection by Desorption Electrospray Ionization-Mass Spectrometry10.1073/pnas.1706459114 ↗
10. Jarmusch, A.K., Alfaro, C.M., Pirro, V., Hattab, E.M., Cohen-Gadol, A.A., and Cooks, R.G. (2016). Differential Lipid Profiles of Normal Human Brain Matter and Gliomas by Positive and Negative Mode Desorption Electrospray Ionization–Mass Spectrometry Imaging. PLoS ONE, 11.10.1371/journal.pone.0163180 ↗
11. A Novel Methodology for in Vivo Endoscopic Phenotyping of Colorectal Cancer Based on Real-Time Analysis of the Mucosal Lipidome: A Prospective Observational Study of the IKnife10.1007/s00464-016-5121-5 ↗
12. MALDI Imaging Mass Spectrometry of Human Tissue: Method Challenges and Clinical Perspectives10.1016/j.tibtech.2010.12.002 ↗
13. A Concise Review of Mass Spectrometry Imaging10.1016/j.chroma.2010.01.033 ↗
14. Heterogeneity of Paclitaxel Distribution in Different Tumor Models Assessed by MALDI Mass Spectrometry Imaging10.1038/srep39284 ↗
15. Ambient Mass Spectrometry10.1126/science.1119426 ↗
16. Mass Spectrometry: Recent Advances in Direct Open Air Surface Sampling/Ionization10.1021/cr300309q ↗
17. Mass Spectrometry Sampling under Ambient Conditions with Desorption Electrospray Ionization10.1126/science.1104404 ↗
18. In Vivo, in Situ Tissue Analysis Using Rapid Evaporative Ionization Mass Spectrometry10.1002/anie.200902546 ↗
19. Rapid Evaporative Ionisation Mass Spectrometry of Electrosurgical Vapours for the Identification of Breast Pathology: Towards an Intelligent Knife for Breast Cancer Surgery10.1186/s13058-017-0845-2 ↗
20. Rapid Diagnosis and Tumor Margin Assessment during Pancreatic Cancer Surgery with the MasSpec Pen Technology10.1073/pnas.2104411118 ↗
21. Probe Electrospray Ionization (PESI) and Its Modified Versions: Dipping PESI (DPESI), Sheath-Flow PESI (SfPESI) and Adjustable SfPESI (Ad-SfPESI)10.5702/massspectrometry.A0092 ↗
22. Physical Properties of the Probe Electrospray Ionization (PESI) Needle Applied to the Biological Samples10.1002/jms.1576 ↗
23. Real-Time Diagnosis of Chemically Induced Hepatocellular Carcinoma Using a Novel Mass Spectrometry-Based Technique10.1016/j.ab.2013.06.017 ↗
24. Rapid Automated Diagnosis of Primary Hepatic Tumour by Mass Spectrometry and Artificial Intelligence10.1111/liv.14604 ↗
25. Prediction of Pathological and Radiological Nature of Glioma by Mass Spectrometry Combined With Machine Learning10.1093/neuopn/okaa026 ↗
26. Innovations in Analytical Oncology—Status Quo of Mass Spectrometry-Based Diagnostics for Malignant Tumor10.6000/1927-7229.2012.01.01.11 ↗
27. Breast Cancer Diagnosis Based on Lipid Profiling by Probe Electrospray Ionization Mass Spectrometry10.1002/bjs.11613 ↗
28. Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
29. WHO Classification of Tumours Editorial Board (2019). Digestive System Tumours, WHO Classification of Tumours Editorial Board.
30. International Consensus Group for Hepatocellular Neoplasia (2009). Pathologic Diagnosis of Early Hepatocellular Carcinoma: A Report of the International Consensus Group for Hepatocellular Neoplasia. Hepatology, 49, 658–664.10.1002/hep.22709 ↗
31. Diagnostic Value of HSP70, Glypican 3, and Glutamine Synthetase in Hepatocellular Nodules in Cirrhosis10.1002/hep.21531 ↗
32. Agreement and Error Rates Using Blinded Review to Evaluate Surgical Pathology of Biopsy Material10.1309/DCXAXFVCCHVHYU41 ↗
33. Measuring Errors in Surgical Pathology in Real-Life Practice: Defining What Does and Does Not Matter10.1309/5KF89P63F4F6EUHB ↗