The Ameliorating Effect of Lizhong-Tang on Antibiotic-Associated Imbalance in the Gut Microbiota in Mouse
Source abstract
Abstract
Some herbal medicines have anti-inflammatory and anti-diarrheal effects. This study analyzed the modulating effect of gut microbiota of anti-inflammatory herbal medicines on antibiotic-associated diarrhea (AAD). The anti-inflammatory effects of 10 herbal medicines and Lizhong-tang active compounds were studied by measuring the nitric oxide production in an in vitro experiment. This was followed by an in vivo experiment in which the anti-diarrheal effects of Lizhong-tang and Magnolia officinalis in a lincomycin-induced AAD mouse model were measured. Changes in the intestinal microflora were observed using terminal restriction fragment length polymorphism analysis. Both Lizhong-tang and M. officinalis were effective against AAD, with Lizhong-tang’s anti-diarrheal effects being particularly effective. In addition, the active compounds of Lizhong-tang, liquiritin and 6-gingerol, inhibited the expression of inducible nitric oxide synthase and cyclooxygenase-2, thus showing an anti-inflammatory effect. Gut microbiota analysis showed that Lizhong-tang could alter the composition of the gut microbiota and ameliorated imbalance in the gut microbiota in a lincomycin-induced AAD mouse model.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Gut microbiota and health · Pharmacological Effects of Natural Compounds · Ginger and Zingiberaceae research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pediatrics, College of Korean Medicine, Daejeon University, Daejeon 34520, Korea
College of Korean Medicine, Gachon University, Seongnam 13120, Korea
Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Korea
College of Pharmacy, Wonkwang University, Iksan 54538, Korea
Funding
the Daejeon University fund (2021) · the Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Education
References (51)
Show source-provided references
1. The NIH human microbiome project10.1101/gr.096651.109 ↗
2. The intestinal microbiota and human disease10.4166/kjg.2013.62.2.85 ↗
3. Development of the gut microbiota in infancy and its impact on health in later life10.1016/j.alit.2017.07.010 ↗
4. Succession of microbial consortia in the developing infant gut microbiome10.1073/pnas.1000081107 ↗
5. Prenatal exposure to antibiotics, cesarean section and risk of childhood obesity10.1038/ijo.2014.180 ↗
6. How nutrition and the maternal microbiota shape the neonatal immune system10.1038/nri.2017.58 ↗
7. The relationship between children’s temperament and character to functional gastrointestinal disorders10.7778/jpkm.2015.29.1.060 ↗
8. The role of microbiota and probiotics in stress-induced gastrointestinal damage10.2174/156652408784533779 ↗
9. A systematic review and meta-analysis of misuse of antibiotic therapies in the community10.1016/j.ijantimicag.2005.04.017 ↗
10. Implications of the changing face of Clostridium difficile disease for health care practitioners10.1016/j.ajic.2006.06.004 ↗
11. The epidemic of antibiotic-resistant infections: A call to action for the medical community from the Infectious Diseases Society of America10.1086/524891 ↗
12. OECD (2021, March 01). OECD Health Data. Available online: https://stats.oecd.org/Index.aspx?ThemeTreeId=9.
13. The infant microbiome development: Mom matters10.1016/j.molmed.2014.12.002 ↗
14. The interaction between the gut microbiota and herbal medicines10.1016/j.biopha.2019.109252 ↗
15. Early development of the gut microbiota and immune health10.3390/pathogens3030769 ↗
16. The gut microbiota as an environmental factor that regulates fat storage10.1073/pnas.0407076101 ↗
17. Structural modulation of gut microbiota during alleviation of suckling piglets diarrhoea with herbal formula10.1155/2017/8358151 ↗
18. Effect of Debaryomyces hansenii combined with Qiweibaizhu powder extract on the gut microbiota of antibiotic-treated mice with diarrhea10.1007/s13205-020-2121-x ↗
19. Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrhea10.1016/j.jep.2020.113594 ↗
20. Incomplete recovery and individualized responses of the human distal gut microbiota to repeated antibiotic perturbation10.1073/pnas.1000087107 ↗
21. A Comparative Analysis about Various Editions of Donguibogam
22. Quantification Analysis and Antioxidant Activity of Leejung-tang10.14374/HFS.2013.21.1.177 ↗
23. The impact of the gut microbiota on human health: An integrative view10.1016/j.cell.2012.01.035 ↗
24. A Review of the Experimental Studies on the Modulatory Effect Herbal Medicine on Gut Microbiota
25. Current trends and future directions of gut microbiota and their-derived metabolite study in the pediatric perspective of korean medicine
26. Han, L., Li, T., Du, M., Chang, R., Zhan, B., and Mao, X. (2019). Beneficial effects of potentilla discolor bunge water extract on inflammatory cytokines release and gut microbiota in high-fat diet and streptozotocin-induced type 2 diabetic mice. Nutrients, 11.10.3390/nu11030670 ↗
27. JinQi Jiangtang tablet regulates gut microbiota and improve insulin sensitivity in type 2 diabetes mice10.1155/2019/1872134 ↗
28. Huang-Lian-Jie-Du-decoction ameliorates hyperglycemia and insulin resistant in association with gut microbiota modulation10.3389/fmicb.2018.02380 ↗
29. Gu, W., Yang, M., Bi, Q., Zeng, L.-X., Wang, X., Dong, J.-C., Li, F.-J., Yang, X.-X., Li, J.-P., and Yu, J. (2020). Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats. BMC Complementary Med. Ther., 20.10.1186/s12906-020-02897-5 ↗
30. Traditional Chinese medicine formula Kang Shuai Lao Pian improves obesity, gut dysbiosis, and fecal metabolic disorders in high-fat diet-fed mice10.3389/fphar.2020.00297 ↗
31. Zhang, C., Liu, J., He, X., Sheng, Y., Yang, C., Li, H., Xu, J., Xu, W., and Huang, K. (2019). Caulis spatholobi ameliorates obesity through activating brown adipose tissue and modulating the composition of gut microbiota. Int. J. Mol. Sci., 20.10.3390/ijms20205150 ↗
32. The role of gut microbiota in obesity and utilization of fermented herbal extracts
33. Hussain, A., Yadav, M.K., Bose, S., Wang, J.-H., Lim, D., Song, Y.-K., Ko, S.-G., and Kim, H. (2016). Daesiho-Tang is an effective herbal formulation in attenuation of obesity in mice through alteration of gene expression and modulation of intestinal microbiota. PLoS ONE, 11.10.1371/journal.pone.0165483 ↗
34. Wuji Wan formula ameliorates diarrhea and disordered colonic motility in post-inflammation irritable bowel syndrome rats by modulating the gut microbiota10.3389/fmicb.2017.02307 ↗
35. Tong-Xie-Yao-Fang regulates 5-HT level in diarrhea predominant irritable bowel syndrome through gut microbiota modulation10.3389/fphar.2018.01110 ↗
36. Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition10.1016/j.jep.2020.112715 ↗
37. Sanhuang Shu’ai decoction alleviates DSS-induced ulcerative colitis via regulation of gut microbiota, inflammatory mediators and cytokines10.1016/j.biopha.2020.109934 ↗
38. Effect of long-term intake of ginseng extracts on gut microbiota in rats
39. Probiotics, gut inflammation and barrier function10.1016/j.gtc.2005.05.010 ↗
40. The rise of the mouse, biomedicine’s model mammal10.1126/science.288.5464.248 ↗
41. How many ways can mouse behavioral experiments go wrong? Confounding variables in mouse models of neurodegenerative diseases and how to control them10.1016/S0065-3454(10)41007-4 ↗
42. Normal flora: Diversity and functions
43. Nitric oxide: A physiologic messenger10.7326/0003-4819-120-3-199402010-00009 ↗
44. Nitric oxide: Physiology, pathophysiology and pharmacology
45. LPS induction of gene expression in human monocytes10.1016/S0898-6568(00)00149-2 ↗
46. Gα12 specifically regulates COX-2 induction by sphingosine 1-phosphate: Role for JNK-dependent ubiquitination and degradation of IκBα10.1074/jbc.M606080200 ↗
47. MAP kinases in the immune response10.1146/annurev.immunol.20.091301.131133 ↗
48. The calm mouse: An animal model of stress reduction10.2119/molmed.2012.00053 ↗
49. Immune adaptations that maintain homeostasis with the intestinal microbiota10.1038/nri2710 ↗
50. Intestinal microbiome is related to lifetime antibiotic use in Finnish pre-school children10.1038/ncomms10410 ↗
51. Efficacy of a low-cost, heat-stable oral rotavirus vaccine in Niger10.1056/NEJMoa1609462 ↗