Riceberry Rice Bran Protein Hydrolyzed Fractions Induced Apoptosis, Senescence and G1/S Cell Cycle Arrest in Human Colon Cancer Cell Lines
Source abstract
Abstract
Riceberry rice bran is the part of rice that has been scrubbed out during the coloring process. There are various health benefits to be gained from foods with a high protein content. This work aimed to study the effect of hydrolyzed riceberry rice bran extracts on colon cancer cell lines compared to normal cells. The MTT assay result showed that our extract has lower cytotoxicity effects on normal cells (PCS-201-010, IC50 = 6745 µg/mL) compared to colon cancer cell lines and has a greater effect on metastatic cancer cell lines (SW-620, IC50 = 5468 µg/mL) than non-metastatic cancer cell lines (HT-29, IC50 = 6054 µg/mL). The apoptotic inductive effects of HRBE on SW-620 were observed after 72 h at a maximum rate of 76% and at maximum concentration. According to the result of the cell senescence analysis after 24 h of HRBE treatment, the percentage of HT-29 (86%) expressing SA-β-gal was much higher than that of SW-620 (32%). Consequently, the decrease in the cell population in the S and M/G2 phases indicated cell cycle arrest in HT-29 cells after being treated with HRBE. Focusing on the peptide fraction size of HRBE, the largest (>50 kDa) fraction showed the highest anticancer activity compared to other fractions. In conclusion, the hydrolyzed riceberry rice bran extract induced the apoptosis process in the metastatic cancer cells and induced the senescence process in the non-metastatic cancer cells. This observed information will be useful and applicable for medical research and colon cancer treatment in the future.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Protein Hydrolysis and Bioactive Peptides · Cancer Research and Treatments · Microbial Metabolism and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
Omics Center for Agriculture, Bio Resources, Food and Health, Kasetsart University (OmiKU), Bangkok 10900, Thailand
Funding
Graduate Program Scholarship from the graduate school and Department of Zoology, Faculty of Science, Kasetsart University
References (37)
Show source-provided references
1. DZNep inhibits the proliferation of colon cancer HCT116 cells by inducing senescence and apoptosis10.1016/j.apsb.2015.01.011 ↗
2. Antioxidant activity of gamma-oryzanol: Mechanism of action and its effect on oxidative stability of pharmaceutical oils10.1016/j.ijpharm.2005.05.018 ↗
3. Chemopreventive properties of the bran extracted from a newly-developed Thai rice: The Riceberry10.1016/j.foodchem.2010.09.093 ↗
4. Isolation and characterization of protein fractions from deoiled rice bran10.1007/s00217-008-0945-4 ↗
5. Functional properties of dietary fibre prepared from defatted rice bran10.1016/S0308-8146(99)00145-4 ↗
6. Protein concentrates from unstabilized and stabilized rice bran: Preparation and properties10.1111/j.1365-2621.1995.tb06293.x ↗
7. Enzyme-assisted water-extraction of oil and protein from rice bran
8. Characterization and bioactivities of young rice protein hydrolysates10.1016/j.jcs.2020.103049 ↗
9. Identification of peptide molecular weight from rice bran protein hydrolysate with high antioxidant activity10.1016/j.jcs.2016.04.011 ↗
10. Rodriguez-Saona, L.E., and Wrolstad, R.E. (2001). Extraction, isolation, and purification of anthocyanins. Curr. Protoc. Food Anal. Chem., F1.1.1–F1.1.11.10.1002/0471142913.faf0101s00 ↗
11. Meerloo, J.V., Kaspers, G.J., and Cloos, J. (2011). Cell sensitivity assays: The MTT assay. Cancer Cell Culture, Springer.10.1007/978-1-61779-080-5_20 ↗
12. Fluorescent dyes for cell viability: An application on prefixed conditions10.1007/s004180100249 ↗
13. Bognar, Z., Cseh, A.M., Fekete, K., Antus, C., Bognar, R., Tapodi, A., Ramadan, F.H., Sumegi, B., and Gallyas, F. (2020). Amiodarone’s major metabolite, desethylamiodarone inhibits proliferation of B16-F10 melanoma cells and limits lung metastasis formation in an in vivo experimental model. PLoS ONE, 15.10.1371/journal.pone.0239088 ↗
14. Beyond annexin V: Fluorescence response of cellular membranes to apoptosis10.1007/s10616-012-9481-y ↗
15. Annona muricata leaves induced apoptosis in A549 cells through mitochondrial-mediated pathway and involvement of NF-κB10.1186/1472-6882-14-299 ↗
16. In vitro DNA adduct profiling to mechanistically link red meat consumption to colon cancer promotion10.1039/C6TX00079G ↗
17. Lichota, A., and Gwozdzinski, K. (2018). Anticancer activity of natural compounds from plant and marine environment. Int. J. Mol. Sci., 19.10.3390/ijms19113533 ↗
18. Natural compounds as modulators of cell cycle arrest: Application for anticancer chemotherapies10.2174/1389202917666160808125645 ↗
19. Natural compounds as anticancer agents: Experimental evidence10.5493/wjem.v2.i3.45 ↗
20. Food-derived anticancer peptides: A review10.1007/s10989-020-10063-1 ↗
21. Immunomodulatory and anticancer protein hydrolysates (peptides) from food proteins: A review10.1016/j.foodchem.2017.10.087 ↗
22. Preclinical evidence on the anticancer properties of food peptides10.2174/0929866523666160816152755 ↗
23. Anticancer activity of hydrophobic peptides from soy proteins10.1002/biof.5520120124 ↗
24. p53 in signaling checkpoint arrest or apoptosis10.1016/S0959-437X(96)90004-0 ↗
25. Kannan, A., Hettiarachchy, N., and Narayan, S. (2009). Colon and breast anti-cancer effects of peptide hydrolysates derived from rice bran. Open Bioact. Compd. J., 2.10.2174/1874847300902010017 ↗
26. The use of therapeutic peptides to target and to kill cancer cells10.2174/092986712801661004 ↗
27. A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry10.1007/s10616-016-0015-x ↗
28. Kntayya, S.B., Ibrahim, M.D., Mohd Ain, N., Iori, R., Ioannides, C., and Abdull Razis, A.F. (2018). Induction of apoptosis and cytotoxicity by isothiocyanate sulforaphene in human hepatocarcinoma HepG2 cells. Nutrients, 10.10.3390/nu10060718 ↗
29. Goel, A., and Arnold, C. (2012). Biology and Genetics of Colorectal Cancer and Polyps and Polyposis. Textb. Clin. Gastroenterol. Hepatol., 428–437.10.1002/9781118321386.ch57 ↗
30. Tan, B., Norhaizan, M., Hazilawati, H., and Roselina, K. (2016). Brewers’ rice, a by-product from rice processing, has antiproliferative activity on human colorectal cancer (HT-29) cell line. Int. Food Res. J., 23.
31. Water extract of brewers’ rice induces apoptosis in human colorectal cancer cells via activation of caspase-3 and caspase-8 and downregulates the Wnt/β-catenin downstream signaling pathway in brewers’ rice-treated rats with azoxymethane-induced colon carcinogenesis10.1186/s12906-015-0730-4 ↗
32. Cytotoxic and apoptotic-inducing effects of purple rice extracts and chemotherapeutic drugs on human cancer cell lines10.7314/APJCP.2013.14.11.6541 ↗
33. Behzadi, P. (2020). IL-21 Signaling and Induction of Cytokine Expression in Human Leukemia Cells and Monocytes, IntechOpen.
34. Qian, Y., and Chen, X. (2013). Senescence regulation by the p53 protein family. Cell Senescence, 37–61.10.1007/978-1-62703-239-1_3 ↗
35. Biosynthesis of storage proteins in developing rice seeds10.1104/pp.70.4.1094 ↗
36. Antioxidative properties of protein hydrolysate from defatted peanut kernels treated with esperase
37. Chemopreventive properties of dietary rice bran: Current status and future prospects10.3945/an.112.002303 ↗