Protective Effect of Chemically Characterized Polyphenol-Rich Fraction from Apteranthes europaea (Guss.) Murb. subsp. maroccana (Hook.f.) Plowes on Carbon Tetrachloride-Induced Liver Injury in Mice
Source abstract
Abstract
Apteranthes europaea (Guss.) Murb. subsp. maroccana (Hook.f.) Plowes (A. europaea) is a medicinal plant widely used in traditional medicines to treat various diseases including hepatic pathogenesis. This study was conducted to evaluate the protective effect of chemically characterized polyphenol-rich fraction from A. europaea on carbon tetrachloride-induced liver injury in mice. The chemical characterization of A. europaea polyphenol-rich fraction was carried out using HPLC-DAD (high-performance liquid chromatography (HPLC) with a diode-array detector (DAD)). Carbon tetrachloride (CCl4) was used to induce liver injuries in mice as described in previous works. A polyphenol-rich fraction from A. europaea was used at a dose of 50 mg/Kg to study its hepatoprotective effect. Next, histopathological and biochemical alterations were investigated. The HPLC analysis revealed the presence of several phenolic compounds: gallic acid, methyl gallate, rutin, ferulic acid, and resorcinol. Regarding the mice treated with a polyphenol-rich fraction from A. europaea up to 50 mg/Kg and carbon tetrachloride, no significant biochemical nor histological alterations occurred in their liver; meanwhile, serious biochemical and histopathological changes were noted for liver recovered from the mice treated with carbon tetrachloride only. In conclusion, A. europaea extract is a promising source of hepatoprotective agents against toxic liver injury.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Drug-Induced Hepatotoxicity and Protection · Phytochemistry and Biological Activities · Genomics, phytochemicals, and oxidative stress
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Laboratory of Biotechnology, Health, Agrofood and Environment (LBEAS), Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco
Laboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy, University of Casablanca, B.P 5696, Casablanca 20250, Morocco
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Micro-Analytical Laboratory, Applied Organic Chemistry Department, National Research Center, Dokki, Cairo 12622, Egypt
Laboratory of Inorganic Chemistry, Department of Chemistry, Faculty of Sciences, 00100 Helsinki, Finland
References (29)
Show source-provided references
1. Grant, D.M. (1991). Detoxification Pathways in the Liver. J. Inherit. Metab. Dis., 421–430.10.1007/978-94-011-9749-6_2 ↗
2. Hepato-protective effect of rutin via IL-6/STAT3 pathway in CCl4-induced hepatotoxicity in rats10.1186/s40659-015-0022-y ↗
3. Review of natural products with hepatoprotective effects10.3748/wjg.v20.i40.14787 ↗
4. Antidiabetic potential of Caralluma europaea against alloxan-induced diabetes in mice10.1016/j.sjbs.2018.05.028 ↗
5. Repertory of standard herbal drugs in the Moroccan pharmacopoea10.1016/0378-8741(91)90064-K ↗
6. Dra, L.A., Aghraz, A., Boualy, B., Oubaassine, S., Barakate, M., Markouk, M., and Larhsini, M. (2018). Chemical Characterization and In vitro Antimicrobial Activity of Caralluma europaea Essential Oil and Its Synergistic Potential with Conventional Antibiotics. J. Adv. Med. Pharm. Sci., 1–11.10.9734/jamps/2018/v19i430094 ↗
7. Toxicological Evaluation of the Aqueous Extract of Caralluma europaea and Its Immunomodulatory and Inflammatory Activities10.4103/pr.pr_24_17 ↗
8. Bourhia, M., Slighoua, M., Ibnemoussa, S., Bari, A., Ullah, R., Amaghnouje, A., Di Cristo, F., El Mzibri, M., Calarco, A., and Benbacer, L. (2020). Phytochemical Study on Antioxidant and Antiproliferative Activities of Moroccan Caralluma europaea Extract and Its Bioactive Compound Classes. Evid.-Based Complement. Altern. Med., e8409718.10.1155/2020/8409718 ↗
9. A review on ethnobotany, phytochemistry and pharmacology of plant genus Caralluma R. Br10.1111/jphp.12265 ↗
10. Bellakhdar, J. (1997). La Pharmacopée Marocaine Traditionnelle: Médecine Arabe Ancienne Et Savoirs Populaires-Saint-Etienne, Ibis Press. Persée—Portail des revues scientifiques en SHS.
11. Dra, L.A., Rodrigues, M.J., Da Rosa Neng, N., Nogueira, J.M., Elamine, Y., Aghraz, A., Markouk, M., Larhsini, M., and Custódio, L. (2019). Exploring Caralluma europaea (Guss.) N.E.Br. as a Potential Source of Bioactive Molecules: In Vitro Antioxidant and Antidiabetic Properties, and Phenolic Profile of Crude Extracts and Fractions. Ind. Crops Prod., Available online: https://agris.fao.org/agris-search/search.do?recordID=US201900393756.10.1016/j.indcrop.2019.111527 ↗
12. Antioxidative effect of polyphenol extract prepared from various Chinese teas10.1016/0091-7435(92)90059-Q ↗
13. Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India10.7324/JAPS.2016.60225 ↗
14. Carbon tetrachloride-induced hepatotoxicity and its amelioration by Agaricus blazei Murrill extract in a mouse model
15. Acute toxicity of plants mixture used in traditional treatment of edema and colic renal in Morocco
16. Liver diseases in developing countries10.4254/wjh.v4.i3.66 ↗
17. Burden of liver diseases in the world10.1016/j.jhep.2018.09.014 ↗
18. Saha, P., Talukdar, A.D., Nath, R., Sarker, S.D., Nahar, L., Sahu, J., and Choudhury, M.D. (2019). Role of Natural Phenolics in Hepatoprotection: A Mechanistic Review and Analysis of Regulatory Network of Associated Genes. Front. Pharmacol., 10.10.3389/fphar.2019.00509 ↗
19. Xenobiotic-induced liver injury and fibrosis10.1517/17425255.2012.674511 ↗
20. Hepatoprotective Effect of Pinoresinol on Carbon Tetrachloride–Induced Hepatic Damage in Mice10.1254/jphs.09234FP ↗
21. Two mechanisms of CCl4-induced fatty liver: Lipid peroxidation or covalent binding studied in cultured rat hepatocytes10.3109/10715768709069797 ↗
22. Lin, S.Y., Xu, D., Du, X.X., Ran, C.L., Xu, L., Ren, S.J., Tang, Z.T., Yin, L.Z., He, C.L., and Yuan, Z.X. (2019). Protective Effects of Salidroside against Carbon Tetrachloride (CCl4)-Induced Liver Injury by Initiating Mitochondria to Resist Oxidative Stress in Mice. Int. J. Mol. Sci., 20.10.3390/ijms20133187 ↗
23. Hepatoprotective effect of Caralluma umbellate against acetaminophen induced oxidative stress and liver damage in rat
24. Hepatoprotective effect of gallic acid isolated from Peltiphyllum peltatum against sodium fluoride-induced oxidative stress10.1016/j.indcrop.2012.10.024 ↗
25. Investigation of polyphenol profile, antioxidant activity and hepatoprotective potential of Aconogonon alpinum (All.) Schur roots10.1515/chem-2020-0062 ↗
26. Protective effect of Acacia confusa bark extract and its active compound gallic acid against carbon tetrachloride-induced chronic liver injury in rats10.1016/j.fct.2009.03.021 ↗
27. Differential hepatoprotective mechanisms of rutin and quercetin in CCl4-intoxicated BALB/cN mice10.1038/aps.2012.62 ↗
28. Hepatoprotective Role of Ferulic Acid: A Dose-Dependent Study10.1089/jmf.2004.7.456 ↗
29. Ferulic acid protects against carbon tetrachloride-induced liver injury in mice10.1016/j.tox.2011.01.017 ↗