journal article·2022-03-16Open access

The Novel Strain of Gluconobacter oxydans H32 Isolated from Kombucha as a Proposition of a Starter Culture for Sour Ale Craft Beer Production

Katarzyna Neffe-Skocińska, Marcin Kruk, Iwona Ścibisz, Dorota Zielińska

Applied Sciences· Volume 12· Issue 6

Abstract

Acetic acid bacteria (AAB) has found applications in food technology, including beverages and vinegar. Generally, AAB shows several beneficial properties and has technological usefulness. Properly selected and tested strains of this group of bacteria may constitute a new and interesting solution among starter cultures for functional food. Therefore, the study aimed to develop a sour beer technology, based on the novel strain Gluconobacter oxydans H32. The microbiological, physical-chemical (HPLC method), and sensory (QDP method) quality were determined during 6 months of storage of dark and light beer samples. The AAB count at the beginning of storage was approximately 8 log CFU mL−1, and 6 log CFU mL−1 after 6 months of storage. As a result of the metabolic activity, acetic acid, gluconic acid, and ascorbic acid were detected in the samples. The light beer had a significantly better sensory quality, especially sample BPGL with the addition of G. oxydans H32 starter culture. It was found that it is possible to develop a functional beer with the novel strain Gluconobacter oxydans H32. These Sour Ale craft beers were not only a good source of H32 strain but also its pro-health metabolites.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Microbial metabolism and enzyme function · Fermentation and Sensory Analysis · Microbial Metabolic Engineering and Bioproduction

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Human Nutrition Sciences, Warsaw University of Life Sciences (WULS), Nowoursynowska St. 159C, 02-776 Warszawa, Poland

Institute of Food Sciences, Warsaw University of Life Sciences (WULS), Nowoursynowska St. 159C, 02-776 Warszawa, Poland

References (48)

Show source-provided references
1. Survival of Probiotic Strain Lactobacillus Paracasei L26 during Co-Fermentation with S. Cerevisiae for the Development of a Novel Beer Beverage10.1016/j.fm.2019.04.001 ↗
2. A Technological Solution to Modulate the Aroma Profile during Beer Fermentation10.1007/s11947-018-2099-0 ↗
3. Hindy, S. (2015). Craft Beer Revolution, The: How a Band of Microbrewers Is Transforming the World’s Favorite Drink, St. Martin’s Griffin.
4. Craft Beers Fermented by Potential Probiotic Yeast or Lacticaseibacilli Strains Promote Antidepressant-Like Behavior in Swiss Webster Mice10.1007/s12602-020-09736-6 ↗
5. (2015). Beer Judge Certification Program. Beer Judge Certyfication Program 2015 Style Guidelines—Beer Style Guidelines, BJCP Inc.
6. Suitability of Beer as an Alternative to Classical Fitness Drinks10.12944/CRNFSJ.4.Special-Issue-October.04 ↗
7. Effects of Moderate Beer Consumption on Health10.20960/nh.2286 ↗
8. Antioxidant Profile at the Different Stages of Craft Beers Production: The Role of Phenolic Compounds10.1007/s00217-020-03637-2 ↗
9. Craft Beer: An Overview10.1111/1541-4337.12693 ↗
10. Yeast: The Soul of Beer’s Aroma—A Review of Flavour-Active Esters and Higher Alcohols Produced by the Brewing Yeast10.1007/s00253-013-5470-0 ↗
11. da Chemical equilibrium and enzymatic kinetics of α-amylase interaction with phenolic compounds found in beer
12. An Overview of Selected Specialty Beers: Developments, Challenges and Prospects10.1111/ijfs.12488 ↗
13. Use of Probiotic Strains to Produce Beers by Axenic or Semi-Separated Co-Culture System10.1016/j.fbp.2020.10.001 ↗
14. Anticancer Impacts of Potentially Probiotic Acetic Acid Bacteria Isolated from Traditional Dairy Microbiota10.1016/j.lwt.2014.09.058 ↗
15. Potentially Probiotic Acetic Acid Bacteria Isolation and Identification from Traditional Dairies Microbiota10.1111/ijfs.12718 ↗
16. Influence of Starter Cultures on the Antioxidant Activity of Kombucha Beverage10.1016/j.foodchem.2011.02.048 ↗
17. Food and Agriculture Organization of the United Nations, and World Health Organization (2006). Probiotics in Food: Health and Nutritional Properties and Guidelines for Evaluation, World Health Organization. FAO food and nutrition paper; Food and Agriculture Organization of the United Nations.
18. The International Scientific Association for Probiotics and Prebiotics Consensus Statement on the Scope and Appropriate Use of the Term Probiotic10.1038/nrgastro.2014.66 ↗
19. European Food Safety Authority (2007). Introduction of a Qualified Presumption of Safety (QPS) Approach for Assessment of Selected Microorganisms Referred to EFSA—Opinion of the Scientific Committee. EFSA J., 5, 587.10.2903/j.efsa.2007.587 ↗
20. Physiology of Acetic Acid Bacteria and Their Role in Vinegar and Fermented Beverages10.1111/1541-4337.12440 ↗
21. European Food Safety Authority (2005). Opinion of the Scientific Committee on a Request from EFSA Related to a Generic Approach to the Safety Assessment by EFSA of Microorganisms Used in Food/Feed and the Production of Food/Feed Additives. EFSA J., 3, 226.10.2903/j.efsa.2005.226 ↗
22. Gluconic Acid: Properties, Applications and Microbial Production
23. Updated List of QPS-Recommended Biological Agents for Safety Risk Assessments Carried out by EFSA
24. Isolation and identification of acetic acid bacteria with potential prohealth properties
25. Determination of Organic Acids in Fruits and Vegetables by Liquid Chromatography with Tandem-Mass Spectrometry10.1016/j.foodchem.2011.10.064 ↗
26. Sugars, Organic Acids, Phenolic Composition and Antioxidant Activity of Sweet Cherry (Prunus avium L.)10.1016/j.foodchem.2007.08.004 ↗
27. Monitoring of Monosaccharides, Oligosaccharides, Ethanol and Glycerol during Wort Fermentation by Biosensors, HPLC and Spectrophotometry10.1016/j.foodchem.2012.10.039 ↗
28. An Improved Sample Preparation Method for Quantification of Ascorbic Acid and Dehydroascorbic Acid by HPLC10.1016/j.lwt.2012.02.004 ↗
29. (2016). 2016 Sensory Analysis-Methodology-General Guidance for Establishing a Sensory Profile (Standard No. ISO 13299).
30. (2012). 2012 Sensory Analysis—General Guidelines for the Selection, Training and Monitoring of Selected Assessors and Expert Sensory Assessors (Standard No. ISO 8586).
31. Ale Beer Containing Free and Immobilized Lactobacillus Brevis, a Potential Delivery System for Probiotics10.1186/s43014-021-00051-3 ↗
32. The Role of Interaction between Yeasts and Lactic Acid Bacteria in African Fermented Milks: A Review10.1016/S0168-1605(03)00247-2 ↗
33. Impact of Saccharomyces Cerevisiae on Viability of Probiotic Lactobacillus Rhamnosus in Fermented Milk under Ambient Conditions10.1111/j.1745-4549.2012.00780.x ↗
34. Probiotic Characterization of Indigenous Bacillus Velezensis Strain DU14 Isolated from Apong, a Traditionally Fermented Rice Beer of Assam10.1016/j.bcab.2019.01.046 ↗
35. Acetic acid bacteria—Taxonomy, ecology, and industrial application
36. Overview on Mechanisms of Acetic Acid Resistance in Acetic Acid Bacteria10.1007/s11274-015-1799-0 ↗
37. A Comparative Study of Stout Beer Batch Fermentation Using Free and Microencapsulated Yeasts10.1007/s11947-010-0379-4 ↗
38. Walker, G.M., and Stewart, G.G. (2016). Saccharomyces Cerevisiae in the Production of Fermented Beverages. Beverages, 2.10.3390/beverages2040030 ↗
39. Biotechnological Applications of Acetic Acid Bacteria10.1080/07388550802046749 ↗
40. Importance of Acetic Acid Bacteria in Food Industry10.1016/j.foodcont.2010.11.008 ↗
41. Complete Genome Sequence of the Acetic Acid Bacterium Gluconobacter Oxydans10.1038/nbt1062 ↗
42. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) (2013). Scientific Opinion on Dietary Reference Values for Vitamin C. EFSA J., 11, 3418.10.2903/j.efsa.2013.3418 ↗
43. Changes in Organic Acids during Beer Fermentation
44. Assessment of Organic Acid and Sugar Composition in Apricot, Plumcot, Plum, and Peach during Fruit Development
45. Sokół-Łętowska, A., Kucharska, A.Z., Hodun, G., and Gołba, M. (2020). Chemical Composition of 21 Cultivars of Sour Cherry (Prunus Cerasus) Fruit Cultivated in Poland. Molecules, 25.10.3390/molecules25194587 ↗
46. Quesada-Molina, M., Muñoz-Garach, A., Tinahones, F.J., and Moreno-Indias, I. (2019). A New Perspective on the Health Benefits of Moderate Beer Consumption: Involvement of the Gut Microbiota. Metabolites, 9.10.3390/metabo9110272 ↗
47. Production and Physicochemical Characterization of Craft Beer with Ginger (Zingiber Officinale)10.1590/fst.16518 ↗
48. Review: Gram Negative Bacteria in Brewing10.4236/aim.2016.63020 ↗