Biodegradation of High Concentrations of Aliphatic Hydrocarbons in Soil from a Petroleum Refinery: Implications for Applicability of New Actinobacterial Strains
Source abstract
Abstract
At present, there is great demand for new resistant and metabolically active strains of biodegrading bacteria capable of degrading high concentrations of petroleum pollutants. In this study, we undertook a series of pot-based biodegradation experiments on soil from a petroleum refinery lagoon heavily polluted with aliphatic hydrocarbons (81.6 ± 2.5 g·kg−1 dry weight) and metals. Periodical bioaugmentation with either a mixture of isolated degraders identified as Bacillus sp. and Ochrobactrum sp. or biostimulation with nutrient medium, singly or in combination, did not produce any significant decrease in hydrocarbons, even after 455 days. Inoculation with Gordonia rubripertincta CWB2 and Rhodococcus erythropolis S43 in iron-limited media, however, resulted in a significant decrease in hydrocarbons 45 days after bioaugmentation. These actinobacterial strains, therefore, show significant potential for bioremediation of such highly polluted soils.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Microbial bioremediation and biosurfactants · Chromium effects and bioremediation · Environmental remediation with nanomaterials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Faculty of Environment, Jan Evangelista Purkyně University in Ústí nad Labem, Králova Výšina 3132/7, 400 96 Ústí nad Labem, Czech Republic
Interdisciplinary Ecological Centre Freiberg, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Str. 29, 09599 Freiberg, Germany
Food Security and Safety Niche Area, Faculty of Natural and Agricultural Sciences, North-West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South Africa
Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Bendlova 1409/7, 461 17 Liberec 1, Czech Republic
Microbial Biotechnology, Ruhr University Bochum, Universitätsstr. 150, 44780 Bochum, Germany
Funding
Ministerstvo Průmyslu a Obchodu · Ministerstvo Školství, Mládeže a Tělovýchovy · Deutscher Akademischer Austauschdienst · Federation of European Microbiological Societies · European Social Fund · Bundesministerium für Bildung und Forschung · Forschungszentrum Jülich
References (55)
Show source-provided references
1. Depletion rate analysis of fields and regions: A methodological foundation10.1016/j.fuel.2013.12.024 ↗
2. Giant oil field decline rates and their influence on world oil production10.1016/j.enpol.2009.02.020 ↗
3. Microbial degradation of petroleum hydrocarbons10.1016/j.biortech.2016.10.037 ↗
4. A comprehensive guide of remediation technologies for oil contaminated soil—Present works and future directions10.1016/j.marpolbul.2016.04.023 ↗
5. Current and emerging trends in bioremediation of petrochemical waste: A review10.1080/10643389.2017.1318616 ↗
6. Comprehensive review on toxicity of persistent organic pollutants from petroleum refinery waste and their degradation by microorganisms10.1016/j.chemosphere.2017.09.005 ↗
7. Azubuike, C.C., Chikere, C.B., and Okpokwasili, G.C. (2016). Bioremediation techniques-classification based on site of application: Principles, advantages, limitations and prospects. World J. Microbiol. Biotechnol., 32.10.1007/s11274-016-2137-x ↗
8. Rhizospheric microorganisms as a solution for the recovery of soils contaminated by petroleum: A review10.1016/j.jenvman.2018.01.015 ↗
9. A new look on factors affecting microbial degradation of petroleum hydrocarbon pollutants10.1016/j.ibiod.2017.02.006 ↗
10. Heine, T., Zimmerling, J., Ballmann, A., Kleeberg, S.B., Ruckert, C., Busche, T., Winkler, A., Kalinowski, J., Poetsch, A., and Scholtissek, A. (2018). On the Enigma of Glutathione-Dependent Styrene Degradation in Gordonia rubripertincta CWB2. Appl. Environ. Microbiol., 84.10.1128/AEM.00154-18 ↗
11. Functional adaptation of microbial communities from jet fuel-contaminated soil under bioremediation treatment: Simulation of pollutant rebound10.1111/j.1574-6941.2011.01169.x ↗
12. Emerging chemicals and the evolution of biodegradation capacities and pathways in bacteria10.1016/j.copbio.2013.08.017 ↗
13. Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History10.3390/s120201544 ↗
14. Singh, B.P., Gupta, V.K., and Passari, A.K. (2018). Actinobacteria: Diversity and Biotechnological Applications: New and Future Developments in Microbial Biotechnology and Bioengineering, Elsevier Science.
15. Amoroso, M.J., Benimeli, C.S., and Cuozzo, S.A. (2013). Actinobacteria: Application in Bioremediation and Production of Industrial Enzymes, CRC Press.10.1201/b14776 ↗
16. Complete genome sequence of Gordonia sp YC-JH1, a bacterium efficiently degrading a wide range of phthalic acid esters10.1016/j.jbiotec.2018.05.009 ↗
17. Cleavage of poly(cis-1,4-isoprene) rubber as solid substrate by cultures of Gordonia polyisoprenivorans10.1016/j.nbt.2018.03.002 ↗
18. Gordonia phthalatica sp. nov., a di-n-butyl phthalate-degrading bacterium isolated from activated sludge10.1099/ijsem.0.002430 ↗
19. New clusters of arsenite oxidase and unusual bacterial groups in enrichments from arsenic-contaminated soil10.1007/s00203-011-0777-7 ↗
20. Effect of various chemical oxidation agents on soil microbial communities10.1016/j.cej.2016.12.065 ↗
21. Use of mineral nutrients and surface-active substances in a biodegradation process modulation10.1007/BF02826564 ↗
22. (2018, August 27). Standardization, BS EN 590 Automotive Fuels—Diesel—Requirements and Test Methods. Available online: http://www.envirochem.hu/www.envirochem.hu/documents/EN_590_2009_hhV05.pdf.
23. Direct molecular biological analysis of ammonia oxidising bacteria populations in cultivated soil plots treated with swine manure10.1111/j.1574-6941.1997.tb00390.x ↗
24. Identification and characterization of a FAD-dependent putrescine N-hydroxylase (GorA) from Gordonia rubripertincta CWB210.1016/j.molcatb.2016.08.003 ↗
25. Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria10.1016/j.btre.2015.01.003 ↗
26. Gallium Mobilization in Soil by Bacterial Metallophores10.4028/www.scientific.net/SSP.262.513 ↗
27. Standardization, E.c.f. (2018, August 27). EN 14039 Characterization of Waste—Determination of Hydrocarbon Content in the Range of C10 to C40 by Gas Chromatography. Available online: https://www.sis.se/api/document/preview/39058/.
28. Kuran, P., Troegl, J., Novakova, J., Pilarova, V., Danova, P., Pavlorkova, J., Kozler, J., Novak, F., and Popelka, J. (2014). Biodegradation of Spilled Diesel Fuel in Agricultural Soil: Effect of Humates, Zeolite, and Bioaugmentation. Sci. World J.10.1155/2014/642427 ↗
29. Deep, subsurface microflora after excavation respiration and biomass and its potential role in degradation of fossil organic matter10.1007/s12223-011-0062-9 ↗
30. (2018, August 27). Organization, ISO 23753–1 Soil Quality—Determination of Dehydrogenase Activity in Soils—Part 1: Method Using Triphenyltetrazolium Chloride (TTC). Available online: https://www.evs.ee/preview/iso-23753-1-2005-en.pdf.
31. Improvement of Analytical Measurements within the BCR-Programme: Single and Sequential Extraction Procedures Applied to Soil and Sediment Analysis10.1080/03067319308027618 ↗
32. Speciation of Heavy Metals in Soils and Sediments. An Account of the Improvement and Harmonization of Extraction Techniques Undertaken Under the Auspices of the BCR of the Commission of the European Communities10.1080/03067319308027619 ↗
33. Effects of inorganic and organic amendments on the mobility (leachability) of heavy metals in contaminated soil: A sequential extraction study10.1016/j.geoderma.2010.08.009 ↗
34. (2018, August 27). Organization, ISO 14235 Soil Quality—Determination of Organic Carbon in Soil by Sulfochromic Oxidation. Available online: https://www.evs.ee/preview/iso-14235-1998-en.pdf.
35. Feasibility of Different Bioremediation Strategies for Treatment of Clayey and Silty Soils Recently Polluted with Diesel Hydrocarbons10.1007/s11270-011-1040-1 ↗
36. Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils10.1007/s13762-012-0071-5 ↗
37. Bioaugmentation and biostimulation strategies to improve the effectiveness of bioremediation processes10.1007/s10532-010-9394-4 ↗
38. Assessment of five bioaccessibility assays for predicting the efficacy of petroleum hydrocarbon biodegradation in aged contaminated soils10.1016/j.chemosphere.2010.09.059 ↗
39. Bioremediation of Crude Oil Contaminated Soils: A Black Art or an Engineering Challenge?10.1205/psep.04388 ↗
40. Effects of biostimulation and bioaugmentation on diesel removal and bacterial community10.1016/j.ibiod.2011.09.012 ↗
41. Evaluation of the optimal strategy for ex situ bioremediation of diesel oil-contaminated soil10.1007/s11356-011-0485-5 ↗
42. Microbial activity in reclaimed and unreclaimed post-mining sites near Sokolov (Czech Republic)10.1016/j.ecoleng.2010.01.007 ↗
43. The strengths and weaknesses of Gordonia: A review of an emerging genus with increasing biotechnological potential10.3109/1040841X.2012.668134 ↗
44. Biology of the metabolically diverse genus Gordonia10.1128/AEM.70.6.3195-3204.2004 ↗
45. The Genus Rhodococcus as a source of novel bioactive substances: A review
46. The genus Rhodococcus10.1046/j.1365-2672.1998.00525.x ↗
47. Production and applications of trehalose lipid biosurfactants10.1002/ejlt.200900162 ↗
48. Microbial degradation of four crude oil by biosurfactant producing strain Rhodococcus sp.10.1016/j.biortech.2017.02.007 ↗
49. Gordonia (nocardia) amarae foaming due to biosurfactant production10.2166/wst.2002.0528 ↗
50. Heterobactins: A new class of siderophores from Rhodococcus erythropolis IGTS8 containing both hydroxamate and catecholate donor groups10.1023/A:1016633529461 ↗
51. Structural Characterization of the Heterobactin Siderophores from Rhodococcus erythropolis PR4 and Elucidation of Their Biosynthetic Machinery10.1021/np4006579 ↗
52. Effects of citric acid and the siderophore desferrioxamine B (DFO-B) on the mobility of germanium and rare earth elements in soil and uptake in Phalaris arundinacea10.1080/15226514.2017.1284752 ↗
53. On the Immobilization of Desferrioxamine-Like Siderophores for Selective Metal Binding10.4028/www.scientific.net/SSP.262.517 ↗
54. Genomic Characterization of the Arsenic-Tolerant Actinobacterium, Rhodococcus erythropolis S4310.4028/www.scientific.net/SSP.262.660 ↗
55. Chemistry and biology of siderophores10.1039/b906679a ↗