Comprehensive Assessment of Pesticide Residues in Fruits and Vegetables from Apulia and Basilicata (Southern Italy, 2022–2025) and Related Risk Evaluation
Source abstract
Abstract
In this study, a comprehensive assessment of pesticide residues in fruits, vegetables, and derived products collected between 2022 and 2025 within the Apulia and Basilicata regions (Southern Italy) is reported. The analytical workflow combined QuEChERS extraction with Gas Chromatography coupled to Triple Quadrupole Mass Spectrometry (GC-QqQ-MS/MS) and Ultra-High-Performance Liquid Chromatography coupled to Heated Electrospray Ionization-Quadrupole Orbitrap Mass Spectrometry (UHPLC-HESI-Q-Orbitrap-MS). A total of 198 samples were analyzed, including fruits (51%), vegetables (35%), and processed products (14%). Approximately 60% of the samples originated from large-scale distribution networks (EU and non-EU imports), while 40% were derived from local production in Apulia and Basilicata. Validation parameters for both methods met the SANTE/11312/2021 and Commission Implementing Regulation (EU) 2021/808 performance requirements. Results showed that 76.8% of samples were free of quantifiable pesticides, while 23.2% contained residues below EU maximum limits, confirming high compliance and effective regional agronomic management in Apulia and Basilicata. The estimated daily intake and chronic hazard index values were below 100%, across all population groups, confirming the absence of chronic dietary risk. The integration of GC-QqQ-MS/MS and UHPLC-HESI-Q-Orbitrap-MS platforms ensured comprehensive chemical coverage and high selectivity, providing an effective regional surveillance model aligned with EU “Farm to Fork” food safety purposes.
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Research context
OpenAlex topics: Pesticide Residue Analysis and Safety · Isotope Analysis in Ecology · Pesticide and Herbicide Environmental Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Laboratorio Diossine, PCB e Pesticidi, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy
References (58)
Show source-provided references
1. (2025, July 07). Healthy Diet. Available online: https://www.who.int/news-room/fact-sheets/detail/healthy-diet.
2. World Health Organization (2003). Diet, Nutrition and the Prevention of Chronic Diseases: Report of a Joint WHO/FAO Expert Consultation, World Health Organization.
3. Fruit and Vegetable Consumption as a Preventative Strategy for Non-Communicable Diseases10.1017/S0029665123002161 ↗
4. Zheng, X., Zhang, X., and Zeng, F. (2025). Biological Functions and Health Benefits of Flavonoids in Fruits and Vegetables: A Contemporary Review. Foods, 14.10.3390/foods14020155 ↗
5. Devirgiliis, C., Guberti, E., Mistura, L., and Raffo, A. (2024). Effect of Fruit and Vegetable Consumption on Human Health: An Update of the Literature. Foods, 13.10.3390/foods13193149 ↗
6. A Scientific Review of Pesticides: Classification, Toxicity, Health Effects, Sustainability, and Environmental Impact
7. Pathak, V.M., Verma, V.K., Rawat, B.S., Kaur, B., Babu, N., Sharma, A., Dewali, S., Yadav, M., Kumari, R., and Singh, S. (2022). Current Status of Pesticide Effects on Environment, Human Health and It’s Eco-Friendly Management as Bioremediation: A Comprehensive Review. Front. Microbiol., 13.10.3389/fmicb.2022.962619 ↗
8. Pesticides and Tremor: An Overview of Association, Mechanisms and Confounders10.1016/j.envres.2023.115442 ↗
9. Uwamahoro, C., Jo, J.-H., Jang, S.-I., Jung, E.-J., Lee, W.-J., Bae, J.-W., and Kwon, W.-S. (2024). Assessing the Risks of Pesticide Exposure: Implications for Endocrine Disruption and Male Fertility. Int. J. Mol. Sci., 25.10.3390/ijms25136945 ↗
10. Botnaru, A.A., Lupu, A., Morariu, P.C., Jităreanu, A., Nedelcu, A.H., Morariu, B.A., Anton, E., Di Gioia, M.L., Lupu, V.V., and Dragostin, O.M. (2025). Neurotoxic Effects of Pesticides: Implications for Neurodegenerative and Neurobehavioral Disorders. J. Xenobiotics, 15.10.3390/jox15030083 ↗
11. Melanda, V.S., Galiciolli, M.E.A., Lima, L.S., Figueiredo, B.C., and Oliveira, C.S. (2022). Impact of Pesticides on Cancer and Congenital Malformation: A Systematic Review. Toxics, 10.10.3390/toxics10110676 ↗
12. Damalas, C.A., and Koutroubas, S.D. (2016). Farmers’ Exposure to Pesticides: Toxicity Types and Ways of Prevention. Toxics, 4.10.3390/toxics4010001 ↗
13. Exposure Levels and Health Implications of Fungicides, Neonicotinoid Insecticides, Triazine Herbicides and Their Associated Metabolites in Pregnant Women and Men10.1016/j.envpol.2023.123069 ↗
14. (2026, January 08). Regulation (EC) No 396/2005 of the European Parliament and of the Council of 23 February 2005 on Maximum Residue Levels of Pesticides in or on Food and Feed of Plant and Animal Origin and Amending Council Directive 91/414/EEC (Text with EEA Relevance). Available online: https://eur-lex.europa.eu/eli/reg/2005/396/oj/eng.
15. (2025, July 07). The 2023 European Union Report on Pesticide Residues in Food|EFSA. Available online: https://www.efsa.europa.eu/en/efsajournal/pub/9398.
16. Reducing Chemical Fertilisers under the European Union’s Farm to Fork Strategy: Implications for Italian Processing Tomatoes10.1016/j.jclepro.2025.146070 ↗
17. European Commission, Directorate General for Health and Food Safety, and Arcadia International (2022). Development of Future Scenarios for Sustainable Pesticide Use and Achievement of Pesticide-Use and Risk-Reduction Targets Announced in the Farm to Fork and Biodiversity Strategies by 2030, Publications Office.
18. European Food Safety Authority (EFSA), Josheski, M., Marchese, E., and Pastor, P.M. (2025). National Summary Reports on Pesticide Residue Analyses Performed in 2023. EFSA Support. Publ., 22, 9320E.10.2903/sp.efsa.2025.EN-9320 ↗
19. (2026, January 08). Regolamento di Esecuzione (UE) 2024/989 della Commissione, del 2 Aprile 2024, Relativo a un Programma Coordinato di Controllo Pluriennale dell’Unione per il 2025, il 2026 e il 2027, Destinato a Garantire il Rispetto dei Livelli Massimi di Residui di Antiparassitari e a Valutare l’esposizione dei Consumatori ai Residui di Antiparassitari nei e sui Prodotti Alimentari di Origine Vegetale e Animale e che Abroga il Regolamento di Esecuzione (UE) 2023/731. Available online: https://eur-lex.europa.eu/legal-content/IT/ALL/?uri=CELEX:32024R0989.
20. (2025, July 07). Regulation—EU—2017/625—EN—EUR-Lex. Available online: https://eur-lex.europa.eu/eli/reg/2017/625/oj/eng.
21. European Union (2004). Regulation
22. (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 on Official Controls Performed to Ensure the Verification of Compliance with Feed and Food Law, Animal Health and Animal Welfare Rules. Off. J. Eur. Union, 165, 1-141.
23. European Union Proficiency Tests for Pesticide Residues in Fruit and Vegetables from 2009 to 2016: Overview of the Results and Main Achievements10.1016/j.foodcont.2017.06.020 ↗
24. European Commission (2026). SANTE/11312/2021 V2026—Guidance Document on Analytical Quality Control and Method Validation Procedures for Pesticide Residues Analysis in Food and Feed, European Commission.
25. European Commission, and Directorate-General for Health and Food Safety (SANTE) (2022). Sante/11312/2021—Analytical Quality Control and Method Validation Procedures for Pesticide Residues Analysis in Food and Feed. v.1, European Commission (DG Sante).
26. European Commission, and Directorate-General for Health and Food Safety (SANTE) (2024). Sante/11312/2021—Analytical Quality Control and Method Validation Procedures for Pesticide Residues Analysis in Food and Feed. v.2, European Commission (DG SANTE).
27. Nardelli, V., D’Amico, V., Ingegno, M., Della Rovere, I., Iammarino, M., Casamassima, F., Calitri, A., Nardiello, D., Li, D., and Quinto, M. (2021). Pesticides Contamination of Cereals and Legumes: Monitoring of Samples Marketed in Italy as a Contribution to Risk Assessment. Appl. Sci., 11.10.3390/app11167283 ↗
28. Evaluation of MS2 Workflows in LC-Q-Orbitrap for Pesticide Multi-Residue Methods in Fruits and Vegetables10.1007/s00216-017-0220-2 ↗
29. Non-Target Data Acquisition for Target Analysis (nDATA) of 845 Pesticide Residues in Fruits and Vegetables Using UHPLC/ESI Q-Orbitrap10.1007/s00216-019-01581-z ↗
30. (2018). UNI Ente Italiano Di Normazione (Standard No. UNI EN 15662:2018). Available online: https://store.uni.com/uni-en-15662-2018.
31. Overcoming Matrix Effects Using the Dilution Approach in Multiresidue Methods for Fruits and Vegetables10.1016/j.chroma.2011.07.033 ↗
32. (2017). Testing and Calibration Laboratories (Standard No. ISO/IEC 17025).
33. The Italian IV SCAI Dietary Survey: Main Results on Food Consumption10.1016/j.numecd.2025.103863 ↗
34. Harmonised Collection of National Food Consumption Data in Europe10.1016/j.foodpol.2020.101908 ↗
35. European Food Safety Authority (2014). Guidance on the EU Menu Methodology. EFSA J., 12, 3944.10.2903/j.efsa.2014.3944 ↗
36. Le Donne, C., Ferrari, M., Mistura, L., D’Addezio, L., Comendador Azcarraga, F.J., Martone, D., Piccinelli, R., Sette, S., Catasta, G., and Turrini, A. (2026). The National Food Consumption Survey IV SCAI: Nutrient Intakes and Related Dietary Sources in Italy. Nutrients, 18.10.3390/nu18010088 ↗
37. Pesticide Residues in Fruits from Egyptian Markets: Detection, Compliance, Co-Occurrence, and Health Risk Assessment10.1016/j.jfca.2025.107842 ↗
38. Evaluation of Pesticide Residues in Fruits and Vegetables from the Region of South-Eastern Poland10.1016/j.foodcont.2014.05.039 ↗
39. Quantification of Pesticide Residues in Fruits and Vegetables Sampled in Sicily (Italy) and Assessment of Health Risks10.1016/j.jfca.2025.108573 ↗
40. European Commission (2026). EU Pesticides Database—Active Substances, European Commission.
41. Hazard Index, a Tool for a Long Term Risk Assessment of Pesticide Residues in Some Commodities, a Pilot Study10.1016/j.yrtph.2015.09.016 ↗
42. Göbölös, B., Sebők, R.E., Szabó, G., Tóth, G., Szoboszlay, S., Kriszt, B., Kaszab, E., and Háhn, J. (2024). The Cocktail Effects on the Acute Cytotoxicity of Pesticides and Pharmaceuticals Frequently Detected in the Environment. Toxics, 12.10.3390/toxics12030189 ↗
43. French Agency for Food, Environmental and Occupational Health & Safety (ANSES), France, Regulated Products Assessment Department, Residues and Food Safety Unit, Chatzidimitriou, E., Mienne, A., Pierlot, S., Noel, L., and Sarda, X. (2019). Assessment of Combined Risk to Pesticide Residues through Dietary Exposure. EFSA J., 17, e170910.10.2903/j.efsa.2019.e170910 ↗
44. European Commission (2021). Commission Implementing Regulation
45. (EU) 2021/808 of 22 March 2021 on the Performance of Analytical Methods for Residues of Pharmacologically Active Substances Used in Food-Producing Animals and on the Interpretation of Results as Well as on the Methods to Be Used for Sampling and Repealing Decisions 2002/657/EC and 98/179/EC, European Commission.
46. Wenio, I., Kwiatkowski, D., Derewiaka, D., and Bartosiewicz, I. (2026). Evaluation of Chromatographic Separation, with a Focus on LC-MS/MS, for the Determination of Stereoisomeric Cypermethrin and Other Synthetic Pyrethroids in Apples. Appl. Sci., 16.10.3390/app16020846 ↗
47. Toxic Effect of Alpha Cypermethrin, an Environmental Pollutant, on Myocardial Tissue in Male Wistar Rats10.1007/s11356-019-05336-2 ↗
48. European Food Safety Authority (EFSA) (2025). Statement on MRLs for Alpha-Cypermethrin and Screening of the Existing EU MRLs for Cypermethrin. EFSA J., 23, e9386.10.2903/j.efsa.2025.9386 ↗
49. European Food Safety Authority (EFSA), Bellisai, G., Bernasconi, G., Binaglia, M., Brancato, A., Cabrera, L.C., Castellan, I., Castoldi, A.F., Chiusolo, A., and Crivellente, F. (2023). Review of the Existing Maximum Residue Levels for Cypermethrins According to Article 12 of Regulation (EC) No 396/2005. EFSA J., 21, e07800.10.2903/j.efsa.2023.7800 ↗
50. Effect of Chemically Bonded Stationary Phases, Mobile Phase Composition and Chemical Structure Analytes on Six Synthetic Insecticides Chiral Resolution in High-Performance Liquid Chromatography10.1007/s41207-025-00806-1 ↗
51. Comprehensive Analysis of Toxics (Pesticides, Veterinary Drugs and Mycotoxins) in Food by UHPLC-MS10.1016/j.trac.2014.06.020 ↗
52. Idowu, S.O., Capaldi, N., Zu, L., and Gupta, A.D. (2013). Stockholm Convention on Persistent Organic Pollutants (POPs). Encyclopedia of Corporate Social Responsibility, Springer.10.1007/978-3-642-28036-8 ↗
53. Dieldrin Accumulation, Distribution in Plant Parts and Phytoextraction Potential for Several Plant Species and Cucurbita pepo Varieties10.1016/j.scitotenv.2024.172968 ↗
54. Pang, S., Lin, Z., Li, J., Zhang, Y., Mishra, S., Bhatt, P., and Chen, S. (2022). Microbial Degradation of Aldrin and Dieldrin: Mechanisms and Biochemical Pathways. Front. Microbiol., 13.10.3389/fmicb.2022.713375 ↗
55. Popescu (Stegarus), D.I., Nasture, A.-M., Niculescu, V.-C., Oprita (Cioara), C.M., and Șuțan (Ionescu), N.A. (2025). Pesticide Surveillance in Fruits and Vegetables from Romanian Supply: A Data-Driven Approach. J. Xenobiotics, 15.10.3390/jox15040104 ↗
56. Fungicides: An Overlooked Pesticide Class?10.1021/acs.est.8b04392 ↗
57. Multifaceted Effects of Difenoconazole in Tomato Fruit Ripening: Physiology, Flavour and Nutritional Quality10.1016/j.plaphy.2022.11.015 ↗
58. Łozowicka, B., Kaczyński, P., Jankowska, M., Rutkowska, E., Iwaniuk, P., Konecki, R., Rogowska, W., Zhagyparova, A., Absatarova, D., and Łuniewski, S. (2025). Evaluation of Broad-Spectrum Pesticides Based on Unified Multi-Analytical Procedure in Fruits and Vegetables for Acute Health Risk Assessment. Foods, 14.10.3390/foods14142528 ↗