journal article·2025-12-02Open access

How Many Trials Are Needed for Consistent Clinical Gait Assessment?

Charlend K. Howard, Christopher K. Rhea, Jacquelyn R. Moxey, Kyle Langerhans, Paphawee Prupetkaew, Brittany S. Samulski

Applied Sciences· Volume 15· Issue 23

Abstract

Background: Clinical assessment of gait typically consists of patients walking a few trials at various speeds while the clinician assesses performance. Unfortunately, there is no clear guidance on how gait changes across trials, leaving clinicians uncertain about the optimal number of trials needed to observe consistent (non-variable) performance. To address this issue, we examined gait performance from a large dataset of older adults who participated in a community-based comprehensive fall risk assessment. Methods: Community-dwelling, older adults (n = 340; 70.8 ± 7.4 years; 120 men, 220 women) performed gait trials under two conditions: preferred and maximum walking speed. Individuals were encouraged to complete five trials for both conditions. Consistency between gait trials within each condition was calculated using intraclass correlation (ICC) and standard error of measurement (SEM) analysis. Results: Our data showed the middle three trials had the most consistency compared to the average of 2–5 trials. Conclusions: When performing a clinical gait analysis, the first trial should be used to acclimate the participant to the protocol and not used for analysis. Data should be recorded from the next three trials, which is when gait appears to stabilize. Data from a fifth trial differs from the second trial, potentially indicating fatigue and/or motivation changes, so it is recommended that the gait analysis conclude after the fourth trial.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Balance, Gait, and Falls Prevention · Prosthetics and Rehabilitation Robotics · Cerebral Palsy and Movement Disorders

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Ellmer College of Health Sciences, Old Dominion University, Norfolk, VA 23529, USA

Funding

Optima Health

References (30)

Show source-provided references
1. Verification of validity of gait analysis systems during treadmill walking and running using human pose tracking algorithm10.1016/j.gaitpost.2021.02.006 ↗
2. Gait biomechanical parameters related to falls in the elderly: A systematic review10.3390/biomechanics4010011 ↗
3. Running-related biomechanical risk factors for overuse injuries in distance runners: A systematic review considering injury specificity and the potentials for future research10.1007/s40279-022-01666-3 ↗
4. McAllister, M.J., Chen, A., and Selinger, J.C. (2025). Behavioural energetics in human locomotion: How energy use influences how we move. J. Exp. Biol., 228.10.1242/jeb.248125 ↗
5. Walking speed: The functional vital sign10.1123/japa.2013-0236 ↗
6. Bradypedia: Is gait speed ready for clinical use?10.1007/s12603-009-0245-0 ↗
7. Assessment of gait speed in older adults10.1519/JPT.0000000000000224 ↗
8. Measuring gait speed in the out-patient clinic: Methodology and feasibility10.4187/respcare.02688 ↗
9. Assessing walking speed in clinical research: A systematic review10.1111/j.1365-2753.2007.00917.x ↗
10. Gait variability in people with neurological disorders: A systematic review and meta-analysis10.1016/j.humov.2016.03.010 ↗
11. Minimal clinically important difference for change in comfortable gait speed of adults with pathology: A systematic review10.1111/jep.12158 ↗
12. Gait speed as a measure in geriatric assessment in clinical settings: A systematic review10.1093/gerona/gls174 ↗
13. Updates in motor learning: Implications for physical therapist practice and education10.1093/ptj/pzab250 ↗
14. Stages of motor skill learning10.1385/MN:32:3:205 ↗
15. Santos, P.C.R.d., Barbieri, F.A., Zijdewind, I., Gobbi, L.T.B., Lamoth, C., and Hortobágyi, T. (2019). Effects of experimentally induced fatigue on healthy older adults’ gait: A systematic review. PLoS ONE, 14.10.1371/journal.pone.0226939 ↗
16. Homagain, A., and Ehgoetz Martens, K.A. (2023). Emotional states affect steady state walking performance. PloS One, 18.10.1371/journal.pone.0284308 ↗
17. Intraclass correlations: Uses in assessing rater reliability10.1037/0033-2909.86.2.420 ↗
18. Quantifying test-retest reliability using the intraclass correlation coefficient and the SEM
19. A guideline of selecting and reporting intraclass correlation coefficients for reliability research10.1016/j.jcm.2016.02.012 ↗
20. Stebbins, J., Harrington, M., and Stewart, C. (2023). Clinical gait analysis 1973–2023: Evaluating progress to guide the future. J. Biomech., 160.10.1016/j.jbiomech.2023.111827 ↗
21. Measurement properties of the usual and fast gait speed tests in community-dwelling older adults: A COSMIN-based systematic review10.1093/ageing/afae055 ↗
22. Shinya, M., and Takiyama, K. (2024). Guidelines for balancing the number of trials and the number of subjects to ensure the statistical power to detect variability–implication for gait studies. J. Biomech., 165.10.1016/j.jbiomech.2024.111995 ↗
23. Kuznetsov, N.A., and Rhea, C.K. (2017). Power considerations for the application of detrended fluctuation analysis in gait variability studies. PLoS ONE, 12.10.1371/journal.pone.0174144 ↗
24. Effect of nonstationarities on detrended fluctuation analysis10.1103/PhysRevE.65.041107 ↗
25. Kirchner, M., Schubert, P., Liebherr, M., and Haas, C.T. (2014). Detrended fluctuation analysis and adaptive fractal analysis of stride time data in Parkinson’s disease: Stitching together short gait trials. PLoS ONE, 9.10.1371/journal.pone.0085787 ↗
26. Marmelat, V., Reynolds, N.R., and Hellman, A. (2018). Gait dynamics in Parkinson’s disease: Short gait trials “stitched” together provide different fractal fluctuations compared to longer trials. Front. Physiol., 9.10.3389/fphys.2018.00861 ↗
27. Task difficulty and the time scales of warm-up and motor learning10.1080/00222895.2013.784240 ↗
28. Loss of ‘complexity’ and aging: Potential applications of fractals and chaos theory to senescence10.1001/jama.1992.03480130122036 ↗
29. Aging and the time and frequency structure of force output variability10.1152/japplphysiol.00166.2002 ↗
30. Complexity of human gait pattern at different ages assessed using multiscale entropy: From development to decline10.1016/j.gaitpost.2016.04.001 ↗