journal article·2025-10-07Open access

X-Ray Absorption and Emission Spectroscopy in Pharmaceutical Applications: From Local Atomic Structure Elucidation to Protein-Metal Complex Analysis—A Review

Klaudia Wojtaszek, Krzysztof Tyrała, Ewelina Błońska-Sikora

Applied Sciences· Volume 15· Issue 19

Abstract

X-ray absorption spectroscopy (XAS) and X-ray emission spectroscopy (XES) are analytical techniques enabling precise analysis of the electronic structure and local atomic environment in chemical compounds and materials. Their application spans materials science, chemistry, biology, and environmental sciences, supporting studies on catalytic mechanisms, redox processes, and metal speciation. A key advantage of both techniques is element selectivity, allowing the analysis of specific elements without matrix interference. Their high sensitivity to chemical state and coordination enables determination of oxidation states, electronic configuration, and local geometry. These methods are applicable to solids, liquids, and gases without special sample preparation. Modern XAS and XES studies are typically performed using synchrotron radiation, which provides an intense, monochromatic X-ray source and allows advanced in situ and operando experiments. Sub-techniques such as XANES (X-ray absorption near-edge structure), EXAFS (Extended X-ray Absorption Fine Structure), and RIXS (resonant inelastic X-ray scattering) offer enhanced insights into oxidation states, local structure, and electronic excitations. Despite their broad scientific use, applications in pharmaceutical research remain limited. Nevertheless, recent studies highlight their potential in analyzing crystalline active pharmaceutical ingredients (APIs), drug–biomolecule interactions, and differences in drug activity. This review introduces the fundamental aspects of XAS and XES, with an emphasis on practical considerations for pharmaceutical applications, including experimental design and basic spectral interpretation.
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Research context

OpenAlex topics: X-ray Spectroscopy and Fluorescence Analysis · Enzyme Structure and Function · X-ray Diffraction in Crystallography

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Pharmaceutical Biophysics, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, Poland

Independent Researcher, 30-669 Kraków, Poland

Department of Pharmaceutical Sciences, Collegium Medicum, Jan Kochanowski University of Kielce, IX Wieków Kielc 19a, 25-516 Kielce, Poland

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