journal article·2025-08-07Open access

Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in Eruca sativa Mill Organs at Flowering Stage

Eleonora Pagnotta, Laura Righetti, Gabriele Micheletti, Carla Boga, Annamaria Massafra, Luisa Ugolini, Lorena Malaguti, Roberto Matteo, Federica Nicoletti, Roberto Colombo, Agostino Fricano, Laura Bassolino

Applied Sciences· Volume 15· Issue 15

Abstract

Glucosinolates are secondary metabolites of the Brassicales, playing a role in plant protection and as health-promoting compounds. Here, Na2SO4 was used to modulate the aliphatic glucosinolate content in different organs of Eruca sativa Mill. In flowers, which accumulate the highest amount of glucosinolates, Na2SO4 increased the concentration of glucoraphanin, in roots of glucoerucin and in apical leaves it doubled the amount of dimeric 4-mercaptobutyl glucosinolate. The biosynthetic gene Branched-Chain Aminotransferase 4 was also induced in roots at the highest salt concentration, while in leaves all tested genes biosynthetic genes were downregulated or unaffected. Cytochromes P450 83A1 monooxygenase was downregulated at the highest salt concentration in all organs. Overall, E. sativa is a reliable source of glucosinolates, which can be modulated with Na2SO4.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Genomics, phytochemicals, and oxidative stress · Moringa oleifera research and applications · Nitrogen and Sulfur Effects on Brassica

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops (CREA-CI), 40128 Bologna, Italy

Department of Industrial Chemistry ‘Toso Montanari’, Alma Mater Studiorum Università di Bologna, 40136 Bologna, Italy

Department of Life Science, University of Modena-Reggio Emilia, 41125 Reggio Emilia, Italy

Council for Agricultural Research and Economics, Research Centre for Agriculture and Environment (CREA-AA), 40128 Bologna, Italy

Council for Agricultural Research and Economics, Research Centre for Genomics and Bioinformatics (CREA-GB), 29017 Fiorenzuola d’Arda, Italy

Funding

SUSinCER Project · CARIPLO FOUNDATION

References (53)

Show source-provided references
1. Glucosinolate Structural Diversity, Identification, Chemical Synthesis and Metabolism in Plants10.1016/j.phytochem.2019.112100 ↗
2. Probing for the Presence of Glucosinolates in Three Drypetes spp. (Drypetes euryodes (Hiern) Hutch., Drypetes gossweileri S. Moore, Drypetes laciniata Hutch.) and Two Rinorea spp. (Rinorea subintegrifolia O. Ktze and Rinorea woermanniana (Büttner) Engl.) from Gabon10.1080/14786419.2016.1236099 ↗
3. Chhajed, S., Mostafa, I., He, Y., Abou-Hashem, M., El-Domiaty, M., and Chen, S. (2020). Glucosinolate Biosynthesis and the Glucosinolate–Myrosinase System in Plant Defense. Agronomy, 10.10.3390/agronomy10111786 ↗
4. Chemosystematics of Brassicales10.1016/j.bse.2017.02.011 ↗
5. Glucosinolate Metabolites Required for an Arabidopsis Innate Immune Response10.1126/science.1164627 ↗
6. Promotion of Arabidopsis Immune Responses by a Rhizosphere Fungus via Supply of Pipecolic Acid to Plants and Selective Augment of Phytoalexins10.1007/s11427-022-2238-8 ↗
7. Effect of Short-Term High Temperature on the Accumulation of Glucosinolates in Brassica rapa10.1016/j.plaphy.2021.02.013 ↗
8. Martínez-Ballesta, M., Moreno-Fernández, D.A., Castejón, D., Ochando, C., Morandini, P.A., and Carvajal, M. (2015). The Impact of the Absence of Aliphatic Glucosinolates on Water Transport under Salt Stress in Arabidopsis thaliana. Front. Plant Sci., 6.10.3389/fpls.2015.00524 ↗
9. The Physiological Importance of Glucosinolates on Plant Response to Abiotic Stress in Brassica10.3390/ijms140611607 ↗
10. Glucosinolate Structural Diversity Shapes Recruitment of a Metabolic Network of Leaf-Associated Bacteria10.1038/s41467-024-52679-7 ↗
11. Retrograde Sulfur Flow from Glucosinolates to Cysteine in Arabidopsis thaliana10.1073/pnas.2017890118 ↗
12. Developing Multifunctional Crops by Engineering Brassicaceae Glucosinolate Pathways10.1016/j.xplc.2023.100565 ↗
13. Regulation of Glucosinolate Biosynthesis10.1093/jxb/eraa479 ↗
14. Large-Scale Identification of Novel Transcriptional Regulators of the Aliphatic Glucosinolate Pathway in Arabidopsis10.1093/jxb/erad376 ↗
15. Omics-Based Identification of Arabidopsis Myb Transcription Factors Regulating Aliphatic Glucosinolate Biosynthesis10.1073/pnas.0611629104 ↗
16. Sønderby, I.E., Hansen, B.G., Bjarnholt, N., Ticconi, C., Halkier, B.A., and Kliebenstein, D.J. (2007). A Systems Biology Approach Identifies a R2R3 MYB Gene Subfamily with Distinct and Overlapping Functions in Regulation of Aliphatic Glucosinolates. PLoS ONE, 2.10.1371/journal.pone.0001322 ↗
17. Arabidopsis Basic Helix-Loop-Helix Transcription Factors MYC2, MYC3, and MYC4 Regulate Glucosinolate Biosynthesis, Insect Performance, and Feeding Behavior10.1105/tpc.113.115139 ↗
18. Advancements in Balancing Glucosinolate Production in Plants to Deliver Effective Defense and Promote Human Health
19. Nematode Suppression with Brassicaceous Amendments: Application Based upon Glucosinolate Profiles10.1016/j.soilbio.2003.12.014 ↗
20. Screening Brassicaceous Plants as Biofumigants for Management of Phytophthora cinnamomi Oak Disease10.1111/efp.12287 ↗
21. Testing Eruca sativa Defatted Seed Meal as a Potential Bioherbicide on Selected Weeds and Crops10.1016/j.indcrop.2021.113834 ↗
22. Ugolini, L., Cilia, G., Pagnotta, E., Malaguti, L., Capano, V., Guerra, I., Zavatta, L., Albertazzi, S., Matteo, R., and Lazzeri, L. (2021). Glucosinolate Bioactivation by Apis Mellifera Workers and Its Impact on Nosema Ceranae Infection at the Colony Level. Biomolecules, 11.10.3390/biom11111657 ↗
23. Costa-Pérez, A., Núñez-Gómez, V., Baenas, N., Di Pede, G., Achour, M., Manach, C., Mena, P., Del Rio, D., García-Viguera, C., and Moreno, D.A. (2023). Systematic Review on the Metabolic Interest of Glucosinolates and Their Bioactive Derivatives for Human Health. Nutrients, 15.10.3390/nu15061424 ↗
24. Connolly, E.L., Liu, A.H., Radavelli-Bagatini, S., Shafaei, A., Boyce, M.C., Wood, L.G., McCahon, L., Koch, H., Sim, M., and Hill, C.R. (2024). Cruciferous Vegetables Lower Blood Pressure in Adults with Mildly Elevated Blood Pressure in a Randomized, Controlled, Crossover Trial: The VEgetableS for vaScular hEaLth (VESSEL) Study. BMC Med., 22.10.1186/s12916-024-03577-8 ↗
25. Influence of Exogenous Elicitors on the Production of Secondary Metabolite in Plants: A Review (“VSI: Secondary Metabolites”)10.1016/j.stress.2023.100166 ↗
26. Elicitation: A Biotechnological Tool for Enhanced Production of Secondary Metabolites in Hairy Root Cultures10.1002/elsc.201900058 ↗
27. Ruffoni, B., Savona, M., Pistelli, L., Bassolino, L., Marchioni, I., and Pistelli, L. (2022). Produzione In Vitro Di Metaboliti Secondari. Le Piante Officinali. Produzione e Prima Trasformazione, Edagricole—Edizioni Agricole di New Business Media srl.
28. Unlocking Nature’s Treasure Trove: Biosynthesis and Elicitation of Secondary Metabolites from Plants10.1007/s10725-024-01184-4 ↗
29. Improvement of Glucosinolates by Metabolic Engineering in Brassica Crops10.1007/s42994-021-00057-y ↗
30. Improving the Phytochemical Composition of Broccoli Sprouts by Elicitation10.1016/j.foodchem.2011.03.049 ↗
31. Impact of Chloride (NaCl, KCl) and Sulphate (Na2SO4, K2SO4) Salinity on Glucosinolate Metabolism in Brassica rapa10.1111/jac.12243 ↗
32. Ilahy, R., Tlili, I., Pék, Z., Montefusco, A., Siddiqui, M.W., Homa, F., Hdider, C., R’Him, T., Lajos, H., and Lenucci, M.S. (2020). Pre- and Post-Harvest Factors Affecting Glucosinolate Content in Broccoli. Front. Nutr., 7.10.3389/fnut.2020.00147 ↗
33. Bioactive Compounds from Eruca sativa Seeds10.3390/encyclopedia2040129 ↗
34. Bell, L., Chadwick, M., Puranik, M., Tudor, R., Methven, L., Kennedy, S., and Wagstaff, C. (2020). The Eruca sativa Genome and Transcriptome: A Targeted Analysis of Sulfur Metabolism and Glucosinolate Biosynthesis Pre and Postharvest. Front. Plant Sci., 11.10.3389/fpls.2020.525102 ↗
35. Some Perspectives on Rocket as a Vegetable Crop: A Review
36. Corti, E., Falsini, S., Gonnelli, C., Pieraccini, G., Nako, B., and Papini, A. (2023). Salt-Affected Rocket Plants as a Possible Source of Glucosinolates. Int. J. Mol. Sci., 24.10.3390/ijms24065510 ↗
37. Glucosinolate Biosynthesis in Eruca sativa10.1016/j.plaphy.2016.10.024 ↗
38. CYP83A1 and CYP83B1, Two Nonredundant Cytochrome P450 Enzymes Metabolizing Oximes in the Biosynthesis of Glucosinolates in Arabidopsis10.1104/pp.102.019240 ↗
39. CYP83A1 Is Required for Metabolic Compatibility of Arabidopsis with the Adapted Powdery Mildew Fungus Erysiphe cruciferarum10.1111/nph.12759 ↗
40. (2024). Rapeseed and Rapeseed Meals—Determination of Glucosinolates Content—Method Using High-Performance Liquid Chromatography (Standard No. ISO 9167:2019).
41. Hydroxyl and Methoxyl Derivatives of Benzylglucosinolate in Lepidium Densiflorum with Hydrolysis to Isothiocyanates and Non-Isothiocyanate Products: Substitution Governs Product Type and Mass Spectral Fragmentation10.1021/acs.jafc.7b00529 ↗
42. Development of a Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry Method for the Simultaneous Analysis of Intact Glucosinolates and Isothiocyanates in Brassicaceae Seeds and Functional Foods10.1016/j.chroma.2015.09.001 ↗
43. A New Mathematical Model for Relative Quantification in Real-Time RT–PCR10.1093/nar/29.9.e45 ↗
44. De Mendiburu, F. (Agricolae: Statistical Procedures for Agricultural Research, 2023). Agricolae: Statistical Procedures for Agricultural Research, R Package Version 1.3-6.
45. Wei, T., Simko, V., Wei, T., and Simko, V. (R Package “Corrplot”: Visualization of a Correlation Matrix, 2024). R Package “Corrplot”: Visualization of a Correlation Matrix, Version 0.95.
46. Ullah, M., Batool, M., El-Badri, A.M., Ikram, M., Zheng, L., Khalid, B., El Khalek Abd El Mola Mohamed, I.A., Chang, L., Wang, B., and Kuai, J. (2025). A Comprehensive Report on Glucosinolate Involvement in Abiotic Stress Responses in Brassicaceae Family. Hortic. Plant J., in press.10.1016/j.hpj.2025.01.017 ↗
47. Growing Hardier Crops for Better Health: Salinity Tolerance and the Nutritional Value of Broccoli10.1021/jf802994p ↗
48. Effect of Salt Treatment on the Glucosinolate-Myrosinase System in Thellungiella Salsuginea10.1007/s11104-011-1108-0 ↗
49. Effect of Salinity (NaCl) on Plant Growth, Nutrient Content, and Glucosinolate Hydrolysis Products Trends in Rocket Genotypes10.1016/j.plaphy.2019.05.012 ↗
50. NRT/PTR Transporters Are Essential for Translocation of Glucosinolate Defence Compounds to Seeds10.1038/nature11285 ↗
51. UV-B-Radiation Induced Di- and Polysulfide Derivatives of 4-Mercaptobutyl Glucosinolate from Eruca sativa10.1016/j.jfca.2023.105485 ↗
52. Identification of the Major Glucosinolate (4-Mercaptobutyl Glucosinolate) in Leaves of Eruca sativa L. (Salad Rocket)10.1016/S0031-9422(02)00203-0 ↗
53. Isolation and Structural Elucidation of 4-(β-D-Glucopyranosyldisulfanyl)Butyl Glucosinolate from Leaves of Rocket Salad (Eruca sativa L.) and Its Antioxidative Activity10.1271/bbb.68.2444 ↗