journal article·2025-08-30Open access

Effects of Spinach Addition on the Nutritional Value, Functional Properties, Microstructure and Shelf Life of Lamb Meat Dumplings

Gulnur Nurymkhan, Almagul Nurgazezova, Galiya Tumenova, Zhibek Atambayeva, Nazerke Muslimova, Botakoz Kulushtayeva, Shugyla Zhakupbekova, Laila Bakirova, Aray Kambarova, Eleonora Okuskhanova

Applied Sciences· Volume 15· Issue 17

Abstract

The incorporation of leafy vegetables into meat products offers a promising strategy for enhancing nutritional value and shelf-life while reducing reliance on synthetic additives. This study evaluated the substitution of lamb (Edilbaev breed) with spinach (0%, 10%, 20%, and 30%) in meat dumplings to assess effects on composition, functionality, microbial stability, lipid oxidation, and sensory quality. Spinach addition enriched the products with minerals, vitamins, and dietary fiber while moderating fat and protein content. Functional properties such as water- and fat-binding capacity were improved, contributing to lower cooking losses, and microbiological tests confirmed slower proliferation of spoilage organisms during chilled storage. Moreover, spinach components contributed to improved oxidative stability, as evidenced by lower thiobarbituric acid values and reduced acid numbers, indicating slower lipid oxidation and hydrolysis. Sensory evaluation revealed that substitution up to 20% maintained favorable appearance, texture, and taste, while higher levels diminished acceptability. Overall, incorporating spinach at a 20% substitution level provides an optimal balance of nutritional enhancement, functional performance, microbial and oxidative stability, and sensory acceptance, making it a practical approach for developing healthier lamb-based dumplings with strong potential for consumer acceptance and market application.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Meat and Animal Product Quality · Agriculture Sustainability and Environmental Impact · Animal Nutrition and Physiology

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Food Technology, Shakarim University, Semey 071412, Kazakhstan

Department of Food Security, M.Kozybayev North Kazakhstan University, Petropavlovsk 150000, Kazakhstan

Funding

Ministry of Science and Higher Education of the Republic of Kazakhstan

References (59)

Show source-provided references
1. Meat production industries of Kazakhstan and Russia: Comparative analysis and trends development
2. World Health Organization (WHO), and Food and Agriculture Organization (FAO) (2007). Protein and Amino Acid Requirements in Human Nutrition: Report of a Joint FAO/WHO/UNU Expert Consultation, World Health Organization.
3. Study of Physical and Chemical and Biochemical Indicators Lamb10.29169/1927-5951.2019.09.01.8 ↗
4. Nutritional composition of lamb retail cuts from the carcases of extensively finished lambs10.1016/j.meatsci.2019.04.016 ↗
5. Lecithin and cardiovascular health: A comprehensive review10.1186/s43044-024-00523-0 ↗
6. Biological features of meat productivity formation in sheep10.15407/animbiol25.01.046 ↗
7. Simunović, S., Tomasevic, I., Djordjevic, V.Ž., Baltić, T., Simunovic, S., Ćirić, J., and Djekic, I. (2024). Meat Color, Marbling, and the Evaluation of Defects in Beef and Pork at the Point of Purchase. Appl. Sci., 14.10.3390/app14156797 ↗
8. Effect of germinated wheat (triticum aestivum) on chemical, amino acid and organoleptic properties of meat pate
9. Functional properties of spinach (Spinacia oleracea L.) phytochemicals and bioactives10.1039/C6FO00051G ↗
10. Effects of Cooking Method on the Vitamin and Mineral Contents in Frequently Used Vegetables10.9724/kfcs.2016.32.3.270 ↗
11. Effect of different cooking methods on the content of vitamins and true retention in selected vegetables
12. Replacement of meat by spinach on physicochemical and nutritional properties of chicken burgers10.1111/jfpp.13935 ↗
13. Development of semi-finished meat products using dairy products and spinach Development of meat products with the use of dairy products and spinach
14. Effect of Fermented Spinach as Sources of Pre-Converted Nitrite on Color Development of Cured Pork Loin10.5851/kosfa.2017.37.1.105 ↗
15. Dynamics of Nitrite Content in Fresh Spinach Leaves: Evidence for Nitrite Formation Caused by Microbial Nitrate Reductase Activity
16. Effects of vegetable powders as nitrite alternative in Italian dry fermented sausage
17. Effect of spinach (Spinacia oleracea) and moringa (Moringa oleifera) powder on physico-chemical and sensory attributes of chicken meat balls
18. Quality evaluation of chicken sausages treated with betle and spinach leaves extract under aerobic packaging condition stored at 4 ± 1 °C10.30954/2277-940X.03.2022.17 ↗
19. Antioxidant activity and quality characteristics of the pork emulsion sausage added with spinach powder10.52346/rsr.2019.1.1.24 ↗
20. Characterization of plant-based meat alternatives blended with anthocyanins, chlorophyll, and various edible natural pigments10.1080/10942912.2023.2224533 ↗
21. (2018). Meat and Meat Products. Protein Determination Methods (Standard No. GOST 25011-2017).
22. (2019). Meat and Meat Products. Methods of Fat Determination (Standard No. GOST 23042–2015).
23. (2015). Meat and Meat Products (Standard No. ST RK ISO 2917-2009).
24. Okuskhanova, E., Yessimbekov, Z., Suychinov, A., Rebezov, M., Semenova, N., Rebezov, Y., Gorelik, O., and Zinina, O. (2017). Study of water binding capacity, pH, chemical composition and microstructure of livestock meat and poultry. Annu. Res. Rev. Biol., 14.10.9734/ARRB/2017/34413 ↗
25. Tymoshenko, N.V., Patieva, A.M., Patieva, S.V., Nesterenko, A.A., and Keniz, N.V. (2015). Functional and Technological Properties of Meat, KubGAU, Kuban Agricultural University Press.
26. Determination of vitamins A and E in infant formula and adult/pediatric nutritional formula by HPLC with UV and fluorescence detection: First action 2012.0910.5740/jaoacint.13-203 ↗
27. (1986). Calcium, Copper, Iron, Magnesium, Manganese, Phosphorus, Potassium, Sodium, and Zinc in Infant Formula. Inductively Coupled Plasma Emission Spectroscopic Method (Standard No. AOAC 984.27-1986). Available online: http://www.aoacofficialmethod.org/index.php?main_page=product_info&cPath=1&products_id=2126.
28. (2003). Food Products. Methods for Determining the Number of Mesophilic Aerobic and Facultative Anaerobic Microorganisms (Standard No. GOST 10444.15-94).
29. (2014). Meat and Meat Products. Method for Determination of Acid Number (Standard No. GOST R 55480-2013).
30. (2018). Meat and Meat Products. Method for Determining the Thiobarbituric Acid Value (Standard No. GOST R 55810-2013).
31. Uzakov, Y.M., Kozhakhieva, M.O., and Nurmukhanbetova, D.E. (2020). Study of the chemical composition of mutton and goat meat for the production of high-quality meat delicacies. Product Quality, Technology, and Education. Proceedings of the XV International Scientific and Practical Conference, Magnitogorsk State Technical University named after G.I. Nosov.
32. Meat quality characteristics of lambs of three organically raised breeds10.1016/j.meatsci.2012.03.004 ↗
33. Vitamin B12 Content in Lamb Meat: Effect of Cooking and Freezing Temperatures
34. The quality and mineral composition of the longissimus lumborum and semimembranosus muscles from lambs fed perennial or annual wheat forage with or without lucerne10.1016/j.meatsci.2021.108564 ↗
35. Nutritional and Bioactive Constituents of Antioxidant and Antimicrobial Properties in Spinacia oleracea: A Review10.17576/jsm-2023-5209-08 ↗
36. Molecular changes of meat proteins during processing and their impact on quality and nutritional values10.1146/annurev-food-052720-124932 ↗
37. Boișteanu, P., Manoliu, D., Flocea, E., Ciobanu, M., and Ciobotaru, M. (2025). Dietary fibres in processed meat: A review on nutritional enhancement, technological effects, sensory implications and consumer perception. Foods, 14.10.3390/foods14091459 ↗
38. Barba, F., Gagaoua, M., Pateiro, M., Domínguez, R., Lorenzo, J., and Zhang, W. (2019). A comprehensive review on lipid oxidation in meat and meat products. Antioxidants, 8.10.3390/antiox8100429 ↗
39. Lipid and protein oxidation during cooking in meat of lambs reared indoors and on pasture
40. Relationships between pH values and some physico-chemical and colour traits of quail breast meat
41. Water-holding and water-holding capacity of meat and methods of its determination10.21323/2414-438X-2023-8-1-62-70 ↗
42. The impact of cellulose structure on binding interactions with hemicellulose and pectin10.1007/s10570-013-9965-8 ↗
43. Enhancing the water holding capacity of model meat analogues through marinade composition10.1016/j.jfoodeng.2020.110283 ↗
44. Study of the functional and technological properties of boiled ham products10.31208/2618-7353-2024-28-48-57 ↗
45. Recent Advances in the Utilization of Natural Emulsifiers to Form and Stabilize Emulsions10.1146/annurev-food-030216-030154 ↗
46. Effect of heat treatment on the dietary fibre contents of potato and tomato10.1111/j.1365-2621.1984.tb00372.x ↗
47. Textural properties and quality of meat products containing fruit or vegetable products: A review
48. Influence of dietary vitamin E on the oxidative stability and quality of pig meat10.2527/1995.73103122x ↗
49. Comparative bioavailability of vitamins in human foods sourced from animals and plants10.1080/10408398.2023.2241541 ↗
50. Calcium induced growth, physio-biochemical, antioxidants, osmolytes adjustments and phytoconstituents status in spinach under heat stress10.1016/j.sajb.2022.06.065 ↗
51. British Nutrition Foundation (1995). Iron as a Pro-Oxidant. Iron: Nutritional and Physiological Significance The Report of the British Nutrition Foundation’s Task Force, Springer.
52. (2025, July 02). The Technical Regulation of the Customs Union “On the Safety of Meat and Meat Products” (TR CU 034/2013) Was Adopted by Decision No. 68 of the Council of the Eurasian Economic Commission on October 9, 2013. Available online: http://www.eurasiancommission.org/ru/act/texnreg/deptexreg/tr/Pages/.
53. Replacement of nitrates and nitrites in meat-derived foods through the utilization of coagulase-negative staphylococci: A review10.1016/j.crfs.2024.100731 ↗
54. Lactic acid/tartaric acid-maltitol antibacterial activity against spoilage bacteria and prevention of yellowing and wilting in spinach and oilseed rape
55. Al-Temimi, A., Al-Hilifi, S., and Al-Mossawi, A. (2023). An investigation on glutathione derived from spinach and red cabbage leaves and their effects of adding to meat patties. Saudi J. Biol. Sci., 30.10.1016/j.sjbs.2023.103632 ↗
56. Levkovskaya, E.V., and Muzykina, D.S. (2022). Improving the technology of minced meat semi-finished products by adding plant components. Agro-Industrial Complex: Problems and Prospects for Development, Far Eastern State Agricultural University.10.22450/9785964205517_4_19 ↗
57. Improving the technology of chopped semi-finished products from rabbit meat
58. Khachatryan, K.G., and Gerashchenko, K.A. (2021). Development of a recipe for zraz with the addition of spinach. Current Issues in Processing and Quality Formation of Agricultural Products: Proceedings of the International Scientific Conference, Krasnoyarsk, Russia, 24 November 2021, Krasnoyarsk State Agrarian University.
59. Martínez, L., Bastida, P., Castillo, J., Ros, G., and Nieto, G. (2019). Green Alternatives to Synthetic Antioxidants, Antimicrobials, Nitrates, and Nitrites in Clean Label Spanish Chorizo. Antioxidants, 8.10.3390/antiox8060184 ↗