Postbiotic Potential of Lactic Acid Bacteria Strains in Functional Minimally Processed Oranges
Source abstract
Abstract
The global market for fresh, ready-to-eat products has grown rapidly, leading to some microbiological safety concerns. Lactic acid bacteria (LAB), along with their metabolites, represent a green alternative to chemical preservatives. For this purpose, the cell-free supernatant of LAB strains previously isolated from fruits and their potential antibacterial effects against pathogens commonly found in minimally processed fruit were evaluated. Based on the preliminary results, a mix of cell-free supernatants (CFSs) was applied as a postbiotic solution in minimally processed orange slices packed in a passive atmosphere. Different pathogenic strains were intentionally inoculated to evaluate their antimicrobial effect, and their trend was monitored. Microbiological and physico-chemical analyses were carried out at different times (3, 8, and 10 days) during cold storage. The CFSs obtained from Lactiplantibacillus plantarum and Leuconostoc mesenteroides strains highlighted an antibacterial activity against Escherichia coli and Staphylococcus aureus in both in vitro and in vivo tests, showing a reduction of 1 Log CFU/mL for E. coli and the disappearance of vital S. aureus. In conclusion, the CFSs can be proposed as an eco-friendly biopreservative for orange slices with a clean label, although their stability needs to be evaluated and their limits of application need to be ruled by specific legislation.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Probiotics and Fermented Foods · Fermentation and Sensory Analysis · Food Quality and Safety Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria (CREA), Centro di Ricerca Olivicoltura, Frutticoltura e Agrumicoltura, Corso Savoia 190, 95024 Acireale, Italy
Department of Agriculture, Food and Environment (Di3A), University of Catania, Via Santa Sofia 100, 95123 Catania, Italy
Funding
Italian Ministry of University and Research (MUR)
References (33)
Show source-provided references
1. Quality preferences and pricing of fresh-cut salads in Italy: New evidence from market data10.1108/BFJ-09-2016-0419 ↗
2. Food and Drug Administration (FDA) (2025, April 15). Current Good Manufacturing Practice, Hazard Analysis, and Risk-Based Preventive Controls for Human Food, Available online: http://federalregister.gov/a/2015-21920.
3. Russo, P., Botticella, G., Capozzi, V., Massa, S., Spano, G., and Beneduce, L. (2014). A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157:H7 in Ready-to-Eat Fresh-Cut Products by MPN-QPCR. BioMed Res. Int., 2014.10.1155/2014/608296 ↗
4. Effectiveness of a pomegranate peel extract (PGE) in reducing Listeria monocytogenes in vitro and on fresh-cut pear, apple and melon10.1007/s00217-020-03529-5 ↗
5. The Potential of Postbiotics as a Novel Approach in Food Packaging and Biopreservation: A Systematic Review of the Latest Developments10.1080/10408398.2023.2253909 ↗
6. Aguirre-Garcia, Y.L., Nery-Flores, S.D., Campos-Muzquiz, L.G., Flores-Gallegos, A.C., Palomo-Ligas, L., Ascacio-Valdés, J.A., Sepúlveda-Torres, L., and Rodríguez-Herrera, R. (2024). Lactic Acid Fermentation in the Food Industry and Bio-Preservation of Food. Fermentation, 10.10.3390/fermentation10030168 ↗
7. Obis, D., Paris, M., and Ouwehand, A.C. (2019). The safety of lactic acid bacteria for use in foods. Lactic Acid Bacteria, CRC Press.10.1201/9780429057465-22 ↗
8. The impacts of antimicrobial and antifungal activity of cell-free supernatants from lactic acid bacteria in vitro and foods10.1111/1541-4337.12872 ↗
9. Prebiotic Effects of Citrus Pectic Oligosaccharides10.1080/14786419.2021.1948845 ↗
10. Characterization and growth under different storage temperatures of ropy slime-producing Leuconostoc mesenteroides isolated from cooked meat products10.4315/JFP-19-521 ↗
11. Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers10.1128/AEM.67.8.3450-3454.2001 ↗
12. Taxonomic and Strain-Specific Identification of the Probiotic Strain Lactobacillus rhamnosus 35 within the Lactobacillus casei Group10.1128/AEM.02286-07 ↗
13. Evaluation of lactic acid bacteria strains isolated from fructose-rich environments for their mannitol-production and milk-gelation abilities10.3168/jds.2020-19120 ↗
14. Enterococcus spp. in Ragusano PDO and Pecorino Siciliano cheese types: A snapshot of their antibiotic resistance distribution10.1016/j.fct.2018.07.023 ↗
15. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing10.1128/jcm.33.9.2233-2239.1995 ↗
16. Ben Farhat, L., Romeo, F.V., Foti, P., Russo, N., Randazzo, C.L., Caggia, C., and Abidi, F. (2022). Multi-Functional Potential of Lactic Acid Bacteria Strains and Antimicrobial Effects in Minimally Processed Pomegranate (Punica granatum L. cv Jolly Red) Arils. Microorganisms, 10.10.3390/microorganisms10101876 ↗
17. Characterization of bioactive Leuconostoc mesenteroides producing bacteriocin strains isolated from camel’s and goat’s Algerian raw milks
18. EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) (2012). Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA J., 10, 2740.10.2903/j.efsa.2012.2740 ↗
19. Physiology, growth, and inhibition of microbes in foods
20. Application of mixture design to introduce an optimum cell-free supernatant of multiple-strain mixture (MSM) for Lactobacillus against food-borne pathogens10.1016/j.lwt.2017.05.035 ↗
21. Bangar, S.P., Suri, S., Trif, M., and Ozogul, F. (2022). Organic acids production from lactic acid bacteria: A preservation approach. Food Biosci., 46.10.1016/j.fbio.2022.101615 ↗
22. Formation of lactic, acetic, succinic, propionic, formic and butyric acid by lactic acid bacteria10.1016/j.lwt.2016.06.066 ↗
23. Food and Drug Administration (FDA) (2025, March 28). Guidance for Industry: Guide to Minimize Microbial Food Safety Hazards of Fresh-Cut Fruits and Vegetables, Available online: https://www.fda.gov/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/ProducePlantProducts/ucm064458.htm.
24. Vaccalluzzo, A., Pino, A., De Angelis, M., Bautista-Gallego, J., Romeo, F.V., Foti, P., Caggia, C., and Randazzo, C.L. (2020). Effects of Different Stress Parameters on Growth and on Oleuropein-Degrading Abilities of Lactiplantibacillus plantarum Strains Selected as Tailored Starter Cultures for Naturally Table Olives. Microorganisms, 8.10.3390/microorganisms8101607 ↗
25. Macarisin, D., Sheth, I., Hur, M., Wooten, A., Kwon, H.J., Gao, Z., De Jesus, A., Jurick, W., and Chen, Y. (2019). Survival of outbreak, food, and environmental strains of Listeria monocytogenes on whole apples as affected by cultivar and wax coating. Sci. Rep., 9.10.1038/s41598-019-48597-0 ↗
26. Kho, K., Kadar, A.D., Bani, M.D., Pramanda, I.T., Martin, L., Chrisdianto, M., Pratama, F., and Devanthi, P.V.P. (2024). The Potential of Pediococcus acidilactici Cell-Free Supernatant as a Preservative in Food Packaging Materials. Foods, 13.10.3390/foods13050644 ↗
27. Optimisation of analytical methods for the characterisation of oranges, clementines and citrus hybrids cultivated in spain on the basis of their composition in ascorbic acid, citric acid and major sugars10.1111/ijfs.12289 ↗
28. Roles of APETALA2/ethylene-response factors in regulation of fruit quality10.1080/07352689.2016.1213119 ↗
29. Carstens, C.K., Salazar, J.K., and Darkoh, C. (2019). Multistate Outbreaks of Foodborne Illness in the United States Associated with Fresh Produce from 2010 to 2017. Front. Microbiol., 10.10.3389/fmicb.2019.02667 ↗
30. Antimicrobial activity of Lactobacillus reuteri DPC16 supernatants against selected food borne pathogens10.1007/s11274-010-0543-z ↗
31. Impact of cell-free supernatant of lactic acid bacteria on putrescine and other polyamine formation by foodborne pathogens in ornithine decarboxylase broth10.1021/acs.jafc.5b02410 ↗
32. Lactic acid bacteria isolates from Pla-som, their antimicrobial activities and fermentation properties in Pla-som
33. Effect of yeasts on food quality and safety and possibilities of their inhibition10.1016/j.tifs.2020.11.022 ↗