Alkali-Treated, Nanostructured-Micro-Porous Titanium Surfaces Enhance Osteogenic Differentiation of Adipose Derived Stem Cells
Source abstract
Abstract
Ensuring effective integration between the material of an implant and bone is critical to orthopedic implants’ success in the long term. A major issue with dense materials is the mechanical mismatch between them and the bone, which leads to improper osseointegration. Porous implants have presented a solution to this issue as they are able to retain material properties in addition to decreasing mismatches. In order to make implants more biomimetic and to match the micro-/nano hierarchy of bone, several surface modifications have been explored in the literature. Hydrothermal treatment in an alkali media on dense titanium has demonstrated higher differentiation of adipose-derived stem cells to osteogenic lineages. In this study, we fabricated nanostructures using hydrothermal treatment in an alkali medium on micro-porous titanium surfaces and evaluated the adhesion, proliferation, and differentiation of adipose derived stem cells to osteoblasts. The nanostructured-micro-porous titanium surfaces displayed enhanced osteogenic differentiation of adipose derived stem cells. Therefore, they have the potential to be used as surfaces for the fabrication of orthopedic implants.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Graphene and Nanomaterials Applications · Electrospun Nanofibers in Biomedical Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Mechanical Engineering, Colorado State University, Fort Collins, CO 80523, USA
School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA
Department of Bioengineering, George Mason University, Fairfax, VA 22030, USA
Funding
National Heart, Lung, and Blood Institute of the National Institute of Health
References (43)
Show source-provided references
1. A state-of-the-art review of the fabrication and characteristics of titanium and its alloys for biomedical applications10.1007/s42242-021-00170-3 ↗
2. Wang, Q., Zhou, P., Liu, S., Attarilar, S., Ma, R.L.-W., Zhong, Y., and Wang, L. (2020). Multi-Scale Surface Treatments of Titanium Implants for Rapid Osseointegration: A Review. Nanomaterials, 10.10.3390/nano10061244 ↗
3. Advancing dental implant surface technology—From micron- to nanotopography10.1016/j.biomaterials.2008.05.012 ↗
4. Self-assembled three-dimensional hierarchical porous V2O5/graphene hybrid aerogels for supercapacitors with high energy density and long cycle life10.1039/C4TA05537C ↗
5. Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration10.1021/acsami.9b04283 ↗
6. Tissue engineering of bone on micropatterned biodegradable polyester films10.1016/j.biomaterials.2005.07.001 ↗
7. Biomimetic microtopography to enhance osteogenesis in vitro10.1016/j.actbio.2011.03.026 ↗
8. Influence of Surface Chemistry on Adhesion and Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells10.1021/acsbiomaterials.7b00274 ↗
9. Tantalum-incorporated hydroxyapatite coating on titanium implants: Its mechanical and in vitro osteogenic properties10.1007/s10856-019-6308-9 ↗
10. Effect of Silver-Containing Titania Layers for Bioactivity, Antibacterial Activity, and Osteogenic Differentiation of Human Mesenchymal Stem Cells on Ti Metal10.1021/acsabm.9b00420 ↗
11. Savargaonkar, A.V., Munshi, A.H., Soares, P., and Popat, K.C. (2023). Antifouling Behavior of Copper-Modified Titania Nanotube Surfaces. J. Funct. Biomater., 14.10.3390/jfb14080413 ↗
12. Strontium delivery on topographical titanium to enhance bioactivity and osseointegration in osteoporotic rats10.1039/C5TB00128E ↗
13. Wolf-Brandstetter, C., Beutner, R., Hess, R., Bierbaum, S., Wagner, K., Scharnweber, D., Gbureck, U., and Moseke, C. (2020). Multifunctional calcium phosphate based coatings on titanium implants with integrated trace elements. Biomed. Mater., 15.10.1088/1748-605X/ab5d7b ↗
14. Enhanced osteoblast functions on anodized titanium with nanotube-like structures10.1002/jbm.a.31551 ↗
15. Biodegradable poly(ε-caprolactone) nanowires for bone tissue engineering applications10.1016/j.biomaterials.2008.10.022 ↗
16. The Topographic Effect of Zinc Oxide Nanoflowers on Osteoblast Growth and Osseointegration10.1002/adma.201002255 ↗
17. High loads of nano-hydroxyapatite/graphene nanoribbon composites guided bone regeneration using an osteoporotic animal model10.2147/IJN.S192456 ↗
18. Fabrication of novel TiZr alloy foams for biomedical applications10.1016/j.msec.2005.08.006 ↗
19. Development and mechanical characterization of porous titanium bone substitutes10.1016/j.jmbbm.2012.01.008 ↗
20. The enhanced effect of surface microstructured porous titanium on adhesion and osteoblastic differentiation of mesenchymal stem cells10.1007/s10856-013-4976-4 ↗
21. Current Progress in Bioactive Ceramic Scaffolds for Bone Repair and Regeneration10.3390/ijms15034714 ↗
22. Biological performance of uncoated and octacalcium phosphate-coated Ti6Al4V10.1016/j.biomaterials.2004.02.026 ↗
23. Osteoinduction of porous bioactive titanium metal10.1016/S0142-9612(03)00551-9 ↗
24. The design of novel nanostructures on titanium by solution chemistry for an improvedosteoblast response10.1088/0957-4484/20/19/195101 ↗
25. Hydrothermal treatment of etched titanium: A potential surface nano-modification technique for enhanced biocompatibility10.1016/j.nano.2019.102016 ↗
26. Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces10.1016/j.carbpol.2020.117079 ↗
27. (2023, October 26). CellTiter-Blue® Cell Viability Assay. Available online: https://www.promega.com/products/cell-health-assays/cell-viability-and-cytotoxicity-assays/celltiter_blue-cell-viability-assay/?catNum=G8080.
28. Savargaonkar, A.V., Holloway, E., Madruga, L.Y.C., Pereira, B.L., Soares, P., and Popat, K.C. (2025). Anti-Bacterial Properties and Hemocompatibility of Alkali Treated Nano-Structured Micro-Porous Titanium Surfaces. Biomimetics, 10.10.3390/biomimetics10020115 ↗
29. Endothelial and smooth muscle cell interaction with hydrothermally treated titanium surfaces10.1007/s44164-024-00073-4 ↗
30. Carboxymethyl-kappa-carrageenan: A study of biocompatibility, antioxidant and antibacterial activities10.1016/j.ijbiomac.2020.02.274 ↗
31. Porous Titanium Implants: A Review10.1002/adem.201700648 ↗
32. Influence of pore size of porous titanium fabricated by vacuum diffusion bonding of titanium meshes on cell penetration and bone ingrowth10.1016/j.actbio.2016.01.022 ↗
33. Effect of crystalline phases of titania nanotube arrays on adipose derived stem cell adhesion and proliferation10.1016/j.msec.2019.109850 ↗
34. Albuschies, J., and Vogel, V. (2013). The role of filopodia in the recognition of nanotopographies. Sci. Rep., 3.10.1038/srep01658 ↗
35. Investigation of the limits of nanoscale filopodial interactions10.1177/2041731414536177 ↗
36. MEM α Promotes Cell Proliferation and Expression of Bone Marrow Derived Equine Mesenchymal Stem Cell Gene Markers but Depresses Differentiation Gene Markers10.1016/j.jevs.2016.10.017 ↗
37. Effects of dexamethasone, ascorbic acid and β-glycerophosphate on the osteogenic differentiation of stem cells in vitro10.1186/scrt328 ↗
38. Phosphorylation of chitosan to improve osteoinduction of chitosan/xanthan-based scaffolds for periosteal tissue engineering10.1016/j.ijbiomac.2019.12.004 ↗
39. The Sequential Expression Profiles of Growth Factors from Osteroprogenitors to Osteoblasts In Vitro10.1089/ten.2006.0423 ↗
40. Effects of titania nanotube surfaces on osteogenic differentiation of human adipose-derived stem cells10.1016/j.nano.2019.01.008 ↗
41. Nanostructured titanium surfaces fabricated by hydrothermal method: Influence of alkali conditions on the osteogenic performance of implants10.1016/j.msec.2018.08.069 ↗
42. Hydrothermally treated titanium surfaces for enhanced osteogenic differentiation of adipose derived stem cells10.1016/j.msec.2021.112315 ↗
43. Cell Differentiation on Nanoscale Features of a Titanium Surface: Effects of Deposition Time in NaOH Solution10.2485/jhtb.23.63 ↗