Enhancement of Phenylpropanoid Accumulation and Antioxidant Activities of Agastache rugosa Transgenic Hairy Root Cultures by Overexpressing the Maize Lc Transcription Factor
Source abstract
Abstract
Agastache rugosa is also known as Korean mint, and it has numerous health benefits due to its rich source of phenolic compounds. The main objective of this study was to produce a ZmLC-overexpressing transgenic hairy root line via Agrobacterium rhizogenes-mediated transformation. The overexpressing transgenic lines were screened using qRT-PCR after exposure to light conditions. The best hairy root line was selected, and the expression levels of phenylpropanoid biosynthetic pathway genes and phenylpropanoid compound accumulation were analysed using qRT-PCR and HPLC, respectively. In addition, antioxidant activities (RPA, ABTS, and DPPH), total phenolic content, and total flavonoid content were analysed. The ZmLC-overexpressing transgenic line upregulated all the phenylpropanoid pathway genes, which led to the higher accumulation of phenylpropanoid compounds in the transgenic line than in the control line. In addition, the total phenolic and flavonoid content was significantly higher in the transgenic line. The antioxidant activity assay showed that the transgenic hairy root line had significantly higher activity than that of the control lines. Thus, ZmLC positively enhances the phenylpropanoid biosynthetic pathway and antioxidant activities in A. rugosa. The results show that ZmLC can be used to enhance phenylpropanoid compounds and antioxidant activities in transgenic A. rugosa hairy root lines via the genetic engineering approach.
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Research context
OpenAlex topics: Plant tissue culture and regeneration · GABA and Rice Research · Biochemical and biochemical processes
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Biotechnology and Food Technology, Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen 24119, Vietnam
Department of Smart Agriculture Systems, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea
Division of Life Sciences and Convergence Research Center for Insect Vectors, Incheon National University, Yeonsu-gu, Incheon 22012, Republic of Korea
Department of Bio-AI Convergence, Chungnam National University, 99 Daehak-ro, Daejeon 34134, Republic of Korea
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea
Department of Bio-Health Convergence, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea
EuHerb Inc., 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea
Funding
Kangwon National University
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