journal article·2025-08-30Open access

Advances in Hydrogel Film Fabrication and Functional Applications Across Biomedical and Environmental Fields

Alberto Ubaldini, Sara Calistri

Applied Sciences· Volume 15· Issue 17

Abstract

Hydrogel films are a promising class of materials due to their peculiar property of retaining water as well as responding to external stimuli. In contrast with conventional hydrogels, films provide enhanced responsiveness along with greater compliance to be integrated into devices as well as on surfaces. This review is designed to comprehensively explore the many aspects of hydrogel films. It covers the principles of gelation; preparation methods, such as solvent casting, spin coating, and photolithography; and characterization. This review also presents the most common polymers (both natural and synthetic) utilized for the preparation of the hydrogel, the systems, such as nanoparticles, liposomes and hybrid metal–organic structure, that can be used as additives and the aspects related to the biocompatibility of hydrogels. In the second part, this review discusses the potential applications of hydrogel films and the challenges that still need to be overcome. Particular attention is given to biomedical applications, such as drug delivery, wound healing, and tissue engineering, but environmental and agricultural uses are also explored. Finally, this review presents recent examples of real-world applications of hydrogel films and explores the possibility they have for a wide variety of needs.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Hydrogels: synthesis, properties, applications · Electrospun Nanofibers in Biomedical Applications · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, C.R. Bologna, Via dei Mille 21, 40121 Bologna, BO, Italy

Department of Pharmacy and Biotechnology, University of Bologna, 40126 Bologna, BO, Italy

Funding

National Recovery and Resilience Plan · NextGenerationEU (NGEU) · Regione Emilia-Romagna

References (100+)

Show source-provided references
1. Choi, H., Choi, W.-S., and Jeong, J.-O. (2024). A Review of Advanced Hydrogel Applications for Tissue Engineering and Drug Delivery Systems as Biomaterials. Gels, 10.10.3390/gels10110693 ↗
2. A Review of Hydrogel Fiber: Design, Synthesis, Applications, and Futures10.1021/acs.chemrev.5c00159 ↗
3. Hydrogels: Classifications, Fundamental Properties, Applications, and Scopes in Recent Advances in Tissue Engineering and Regenerative Medicine—A Comprehensive Review10.1016/j.arabjc.2024.105968 ↗
4. Delgado-Pujol, E.J., Martínez, G., Casado-Jurado, D., Vázquez, J., León-Barberena, J., Rodríguez-Lucena, D., Torres, Y., Alcudia, A., and Begines, B. (2025). Hydrogels and Nanogels: Pioneering the Future of Advanced Drug Delivery Systems. Pharmaceutics, 17.10.3390/pharmaceutics17020215 ↗
5. Nunes, D., Andrade, S., Ramalho, M.J., Loureiro, J.A., and Pereira, M.C. (2022). Polymeric Nanoparticles-Loaded Hydrogels for Biomedical Applications: A Systematic Review on In Vivo Findings. Polymers, 14.10.3390/polym14051010 ↗
6. Liposomes-in-hydrogel for topical drug delivery: Mechanical, kinetic, and biological insights10.1016/j.jddst.2025.107380 ↗
7. Binaymotlagh, R., Hajareh Haghighi, F., Chronopoulou, L., and Palocci, C. (2024). Liposome–Hydrogel Composites for Controlled Drug Delivery Applications. Gels, 10.10.3390/gels10040284 ↗
8. Yoon, M.S., Lee, J.M., Jo, M.J., Kang, S.J., Yoo, M.K., Park, S.Y., Bong, S., Park, C.-S., Park, C.-W., and Kim, J.-S. (2025). Dual-Drug Delivery Systems Using Hydrogel–Nanoparticle Composites: Recent Advances and Key Applications. Gels, 11.10.3390/gels11070520 ↗
9. Priya, A.S., Premanand, R., Ragupathi, I., Bhaviripudi, V.R., Aepuru, R., Kannan, K., and Shanmugaraj, K. (2024). Comprehensive Review of Hydrogel Synthesis, Characterization, and Emerging Applications. J. Compos. Sci., 8.10.3390/jcs8110457 ↗
10. Sánchez-Cid, P., Jiménez-Rosado, M., Romero, A., and Pérez-Puyana, V. (2022). Novel Trends in Hydrogel Development for Biomedical Applications: A Review. Polymers, 14.10.3390/polym14153023 ↗
11. Current Hydrogel Advances in Physicochemical and Biological Response Driven Biomedical Application Diversity10.1038/s41392-021-00830-x ↗
12. Protsak, I.S., and Morozov, Y.M. (2025). Fundamentals and Advances in Stimuli-Responsive Hydrogels and Their Applications: A Review. Gels, 11.10.3390/gels11010030 ↗
13. Bioactuators Based on Stimulus Responsive Hydrogels and Their Emerging Biomedical Applications10.1038/s41427-019-0165-3 ↗
14. Designing Hydrogels for Controlled Drug Delivery10.1038/natrevmats.2016.71 ↗
15. A Practical Guide to Hydrogels for Cell Culture10.1038/nmeth.3839 ↗
16. Hydrogel Scaffolds for Tissue Engineering: Progress and Challenges
17. Thin Hydrogel Films for Optical Biosensor Applications10.3390/membranes2010040 ↗
18. Highly Sensitive Active Powering Pressure Sensor Enabled by Integration of Double Rough Surface Hydrogel and Flexible Batteries10.1038/s41528-022-00226-z ↗
19. Functional Hydrogel Coatings10.1093/nsr/nwaa254 ↗
20. Niemczyk-Soczynska, B., and Sajkiewicz, P.Ł. (2025). Hydrogel-Based Systems as Smart Food Packaging: A Review. Polymers, 17.10.3390/polym17081005 ↗
21. Hydrogels in Soft Robotics: Past, Present, and Future10.1021/acsnano.3c12200 ↗
22. Smart Materials for Flexible Electronics and Devices: Hydrogel10.1039/D4RA01168F ↗
23. Hu, L., Yang, Y., Yu, W., and Xu, L. (2024). Hydrogels for Lubrication: Synthesis, Properties, Mechanism, and Challenges. Lubricants, 12.10.3390/lubricants12060186 ↗
24. Samchenko, Y., Terpilowski, K., Samchenko, K., Golovkova, L., Oranska, O., and Goncharuk, O. (2024). Calcium Alginate/Laponite Nanocomposite Hydrogels: Synthesis, Swelling, and Sorption Properties. Coatings, 14.10.3390/coatings14121519 ↗
25. Zhang, X., Wang, X., Fan, W., Liu, Y., Wang, Q., and Weng, L. (2022). Fabrication, Property and Application of Calcium Alginate Fiber: A Review. Polymers, 14.10.3390/polym14153227 ↗
26. Calistri, S., Ciantelli, C., Cataldo, S., Cuzzola, V., Guzzinati, R., Busi, S., and Ubaldini, A. (2025). Simple Spin-Coating Preparation of Hydrogel and Nanoparticle-Loaded Hydrogel Thin Films. Coatings, 15.10.3390/coatings15070859 ↗
27. Recent progress in fabrications and applications of functional hydrogel films10.1002/pol.20220451 ↗
28. Narayanaswamy, R., and Torchilin, V.P. (2019). Hydrogels and Their Applications in Targeted Drug Delivery. Molecules, 24.10.3390/molecules24030603 ↗
29. Nanoparticles Incorporated Hydrogels for Delivery of Antimicrobial Agents: Developments and Trends10.1039/D4RA00631C ↗
30. Gelation—A Review10.1002/pi.4990250302 ↗
31. Chopra, H., Bibi, S., Kumar, S., Khan, M.S., Kumar, P., and Singh, I. (2022). Preparation and Evaluation of Chitosan/PVA Based Hydrogel Films Loaded with Honey for Wound Healing Application. Gels, 8.10.3390/gels8020111 ↗
32. Gomes, C.P., Bzainia, A., Almeida, A., Martins, C., Dias, R.C.S., and Costa, M.R.P.F.N. (2024). Chemical Routes for the Transformation of Bio-monomers into Polymers. Plant Biomass Derived Materials: Sources, Extractions, and Applications, Wiley WCH.10.1002/9783527839032.ch13 ↗
33. Perale, G., and Hilborn, J. (2017). Synthesis and Processing of Hydrogels for Medical Applications. Bioresorbable Polymers for Biomedical Applications, Woodhead Publishing.
34. Competition Among Physical, Chemical, and Hybrid Gelation Mechanisms in Biopolymers10.1039/D3SM01682J ↗
35. Spontaneous Synthesis of a Homogeneous Thermoresponsive Polymer Network Composed of Polymers with a Narrow Molecular Weight Distribution10.1038/s41427-018-0074-x ↗
36. Formation of Calcium Alginate Gel Capsules: Influence of Sodium Alginate and CaCl2 Concentration on Gelation Kinetics10.1016/S1389-1723(00)87103-0 ↗
37. Călina, I., Demeter, M., Scărișoreanu, A., Abbas, A., and Raza, M.A. (2025). Role of Ionizing Radiation Techniques in Polymeric Hydrogel Synthesis for Tissue Engineering Applications. Gels, 11.10.3390/gels11010047 ↗
38. Yammine, P., El Safadi, A., Kassab, R., El-Nakat, H., Obeid, P.J., Nasr, Z., Tannous, T., Sari-Chmayssem, N., Mansour, A., and Chmayssem, A. (2025). Types of Crosslinkers and Their Applications in Biomaterials and Biomembranes. Chemistry, 7.10.3390/chemistry7020061 ↗
39. Design of dual stimuli-responsive gels with physical and chemical properties that vary in response to light and temperature and cell behavior on their surfaces10.1038/s41428-023-00865-7 ↗
40. In Situ Cross-Linkable Hydrogels as a Dynamic Matrix for Tissue Regenerative Medicine10.1007/s13770-018-0155-5 ↗
41. Post-synthesis modification of hydrogels. Total and partial rupture of crosslinks: Formation of aldehyde groups and re-crosslinking of cleaved hydrogels10.1016/j.polymer.2017.03.068 ↗
42. Ahmed, M.S., Islam, M., Hasan, M.K., and Nam, K.W. (2024). A Comprehensive Review of Radiation-Induced Hydrogels: Synthesis, Properties, and Multidimensional Applications. Gels, 10.10.3390/gels10060381 ↗
43. Adhesion mechanism and application progress of hydrogels10.1016/j.eurpolymj.2022.111277 ↗
44. Microwave-Assisted Hydrogel Synthesis: A New Method for Crosslinking Polymers in Aqueous Solutions10.1002/marc.201100742 ↗
45. Microwave-Assisted Preparation of Hydrogel-Forming Microneedle Arrays for Transdermal Drug Delivery Applications10.1002/mame.201500016 ↗
46. Sun, P., Li, X., Kong, B., Zhu, Y., Wang, M., Wang, H., and Liu, Q. (2023). Fabrication and characterization of microwave-assisted synthesis of carbon dots crosslinked sodium alginate hydrogel films. Int. J. Biol. Macromol., 253.10.1016/j.ijbiomac.2023.127130 ↗
47. A Facile Synthesis of Self-Catalytic Hydrogel Films and Their Application as a Wound Dressing Material Coupled with Natural Active Compounds10.1021/acsomega.0c03414 ↗
48. Mali, K.K., Ghorpade, V.S., Dias, R.J., and Dhawale, S.C. (2023). Synthesis and characterization of citric acid crosslinked carboxymethyl tamarind gum-polyvinyl alcohol hydrogel films. Int. J. Biol. Macromol., 236.10.1016/j.ijbiomac.2023.123969 ↗
49. Review of sol–gel thin film formation10.1016/S0022-3093(05)80653-2 ↗
50. Spin-coating-assisted fabrication of ultrathin physical hydrogel films with high toughness and fast response10.1039/C8SM01126E ↗
51. Tough and Functional Hydrogel Coating by Electrostatic Spraying10.1002/smll.202408780 ↗
52. Lignin-Based Ultrathin Hydrogel Coatings with Strong Substrate Adhesion Enabled by Hydrophobic Association10.1002/adfm.202413464 ↗
53. Towards Large-Scale Fabrication of Self-Healable Functional Hydrogel Coatings for Anti-Fog/Frost Surfaces and Flexible Sensors10.1002/admt.202001267 ↗
54. Pemble, O.J., Bardosova, M., Povey, I.M., and Pemble, M.E. (2021). A Slot-Die Technique for the Preparation of Continuous, High-Area, Chitosan-Based Thin Films. Polymers, 13.10.3390/polym13101566 ↗
55. Photolithographically Patterned Hydrogels with Programmed Deformations10.1002/asia.201801333 ↗
56. Zhang, Y., and Wang, C. (2022). Recent advances in 3D printing hydrogel for topical drug delivery. MedComm—Biomater. Appl., 1.10.1002/mba2.11 ↗
57. Thin films as an emerging platform for drug delivery
58. Critical review of polymer and hydrogel deposition methods for optical and electrochemical bioanalytical sensors correlated to the sensor’s applicability in real samples10.1007/s00216-022-04363-2 ↗
59. Sprayable porous hydrogel coating for efficient and sustainable evaporative cooling10.1016/j.matt.2024.08.016 ↗
60. Butt, M.A. (2022). Thin-Film Coating Methods: A Successful Marriage of High-Quality and Cost-Effectiveness—A Brief Exploration. Coatings, 12.10.20944/preprints202207.0177.v1 ↗
61. Scaled Production of Functionally Gradient Thin Films Using Slot Die Coating on a Roll-to-Roll System10.1021/acsami.3c17558 ↗
62. Optofluidic thin-film lithography for photocrosslinking hydrogel-based microarchitectures and the assembling of modular cell-embedded microarchitectures10.1016/j.snb.2021.131048 ↗
63. 3D printing of polymer composites: Materials, processes, and applications10.1016/j.matt.2021.10.018 ↗
64. Methods of synthesis of hydrogels…A review10.1016/j.jsps.2015.03.022 ↗
65. Analysis of Local Viscosity in Alginate Gel via Bead-based Diffusometry
66. 3D Printing-Based Hydrogel Dressings for Wound Healing10.1002/advs.202404580 ↗
67. Kaliaraj, G.S., Shanmugam, D.K., Dasan, A., and Mosas, K.K.A. (2023). Hydrogels—A Promising Materials for 3D Printing Technology. Gels, 9.10.3390/gels9030260 ↗
68. Rajendran, A.K., and Jayakumar, R. (2025). Preparation of hydrogels for biomedical applications: Biomimetic approach. Woodhead Publishing Series in Biomaterials, Hydrogel Tissue Analogues, Woodhead Publishing.10.1016/B978-0-443-29260-6.00008-1 ↗
69. A simple soft lithographic route to fabrication of poly(ethylene glycol) microstructures for protein and cell patterning10.1016/S0142-9612(03)00543-X ↗
70. Aghajani, M., Garshasbi, H.R., Naghib, S.M., and Mozafari, M.R. (2025). 3D Printing of Hydrogel Polysaccharides for Biomedical Applications: A Review. Biomedicines, 13.10.3390/biomedicines13030731 ↗
71. Prospects of Hydrogels in Agriculture for Enhancing Crop and Water Productivity under Water Deficit Condition10.1155/2022/4914836 ↗
72. A robust and transparent hydrogel coating for sustainable antifogging with excellent self-cleaning and self-healing ability10.1016/j.cej.2022.137879 ↗
73. Decoupling individual optical nanosensor responses using a spin-coated hydrogel platform10.1021/acsami.2c16596 ↗
74. Blinova, E., Korel, A., Zemlyakova, E., Pestov, A., Samokhin, A., Zelikman, M., Tkachenko, V., Bets, V., Arzhanova, E., and Litvinova, E. (2024). Cytotoxicity and Degradation Resistance of Cryo- and Hydrogels Based on Carboxyethylchitosan at Different pH Values. Gels, 10.10.3390/gels10040272 ↗
75. Hydrogel: Preparation, characterization, and applications: A review10.1016/j.jare.2013.07.006 ↗
76. Chafran, L., Carfagno, A., Altalhi, A., and Bishop, B. (2022). Green Hydrogel Synthesis: Emphasis on Proteomics and Polymer Particle-Protein Interaction. Polymers, 14.10.3390/polym14214755 ↗
77. Bashir, S., Hina, M., Iqbal, J., Rajpar, A.H., Mujtaba, M.A., Alghamdi, N.A., Wageh, S., Ramesh, K., and Ramesh, S. (2020). Fundamental Concepts of Hydrogels: Synthesis, Properties, and Their Applications. Polymers, 12.10.3390/polym12112702 ↗
78. Advances in the application of natural/synthetic hybrid hydrogels in tissue engineering and delivery systems: A comprehensive review10.1016/j.ijpharm.2025.125323 ↗
79. Comprehensive review of natural based hydrogels as an upcoming trend for food packing10.1016/j.foodhyd.2022.108124 ↗
80. Natural polymer-based hydrogels: Types, functionality, food applications, environmental significance and future perspectives: An updated review
81. Sharma, R., Malviya, R., Singh, S., and Prajapati, B. (2023). A Critical Review on Classified Excipient Sodium-Alginate-Based Hydrogels: Modification, Characterization, and Application in Soft Tissue Engineering. Gels, 9.10.3390/gels9050430 ↗
82. A Review on Hydrophobically Associated Alginates: Approaches and Applications10.1021/acsomega.3c08619 ↗
83. Iron(iii)-cross-linked alginate hydrogels: A critical review10.1039/D1MA00959A ↗
84. Xie, Y., Gao, P., He, F., and Zhang, C. (2022). Application of Alginate-Based Hydrogels in Hemostasis. Gels, 8.10.3390/gels8020109 ↗
85. Development of novel alginate based hydrogel films for wound healing applications10.1016/j.ijbiomac.2012.09.031 ↗
86. Chitosan-based hydrogels: From preparation to applications, a review10.1016/j.fochx.2023.101095 ↗
87. Aranaz, I., Alcántara, A.R., Civera, M.C., Arias, C., Elorza, B., Heras Caballero, A., and Acosta, N. (2021). Chitosan: An Overview of Its Properties and Applications. Polymers, 13.10.3390/polym13193256 ↗
88. Chitosan based edible films and coatings: A review10.1016/j.msec.2013.01.010 ↗
89. Drug delivery using interpenetrating polymeric networks of natural polymers: A recent update10.1016/j.jddst.2021.102915 ↗
90. Chapter 4—Polysaccharides of the red algae10.1016/B978-0-12-385520-6.00004-2 ↗
91. Ultrathin κ-Carrageenan/Chitosan Hydrogel Films with High Toughness and Antiadhesion Property10.1021/acsami.7b18343 ↗
92. New insights into biomaterials for wound dressings and care: Challenges and trends10.1016/j.porgcoat.2023.108118 ↗
93. Valachová, K., Hassan, M.E., and Šoltés, L. (2024). Hyaluronan: Sources, Structure, Features and Applications. Molecules, 29.10.3390/molecules29030739 ↗
94. Trombino, S., Servidio, C., Curcio, F., and Cassano, R. (2019). Strategies for Hyaluronic Acid-Based Hydrogel Design in Drug Delivery. Pharmaceutics, 11.10.3390/pharmaceutics11080407 ↗
95. Wu, M., Cronin, K., and Crane, J.S. (2025, August 27). Biochemistry, Collagen Synthesis, StatPearls, Available online: https://www.ncbi.nlm.nih.gov/books/NBK507709/.
96. Collagen structure and stability10.1146/annurev.biochem.77.032207.120833 ↗
97. Gahlawat, S., Nanda, V., and Shreiber, D.I. (2024). Designing collagens to shed light on the multi-scale structure–function mapping of matrix disorders. Matrix Biol. Plus, 21.10.1016/j.mbplus.2023.100139 ↗
98. Li, Y., Dong, X., Yao, L., Wang, Y., Wang, L., Jiang, Z., and Qiu, D. (2023). Preparation and Characterization of Nanocomposite Hydrogels Based on Self-Assembling Collagen and Cellulose Nanocrystals. Polymers, 15.10.3390/polym15051308 ↗
99. Recent Advances in the Synthesis and Applications of Collagen Based Hydrogels: A Review
100. Hu, T., and Lo, A.C.Y. (2021). Collagen-Alginate Composite Hydrogel: Application in Tissue Engineering and Biomedical Sciences. Polymers, 13.10.3390/polym13111852 ↗