journal article·2025-07-23Open access

The Impact of Grafted Larvae and Collection Day on Royal Jelly’s Production and Quality

Dimitrios Kanelis, Vasilios Liolios, Maria Anna Rodopoulou, Fotini Papadopoulou, Chrysoula Tananaki

Applied Sciences· Volume 15· Issue 15

Abstract

Royal jelly (RJ), a secretion from nurse bees, is a key factor in honeybee caste differentiation and a high-value product in apitherapy. Despite its economic and biological importance, factors affecting its yield and composition remain insufficient. This study investigated the impact of grafted larval age and sex and the collection day of RJ on its yield and physicochemical characteristics. Three independent experiments were conducted using strong Apis mellifera L. colonies. Larvae of different ages (first, second, and third) were grafted, and RJ was harvested 24, 48, and 72 h post grafting. Additionally, worker and drone larvae were used to assess the effect of larval sex. RJ was analyzed for moisture, protein, sugar, and 10-hydroxy-2-decenoic acid (10-had) content. Results showed that RJ yield significantly increased with collection day, with the third day being optimal. Protein content declined over time, while moisture content rose, although sugar levels and 10-HDA remained stable. Second-day larvae yielded the highest RJ volume without affecting composition. Larval sex did not significantly influence either RJ yield or composition. The results of this study may provide valuable insights into the quality determinants of royal jelly, enabling beekeepers to optimize production for both enhanced royal jelly yield and the rearing of higher-quality queen bees.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Bee Products Chemical Analysis · Food Quality and Safety Studies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Laboratory of Apiculture-Sericulture, Aristotle University of Thessaloniki, Aristotle Farm, 57001 Thermi, Greece

References (38)

Show source-provided references
1. Buttstedt, A., Moritz, R.F., and Erler, S. (2013). More than royal food-Major royal jelly protein genes in sexuals and workers of the honeybee Apis mellifera. Front. Zool., 10.10.1186/1742-9994-10-72 ↗
2. Royalactin induces queen differentiation in honeybees10.1038/nature10093 ↗
3. Bioactive compounds and health-promoting properties of royal jelly: A review10.1016/j.jff.2011.12.007 ↗
4. Ahmad, S., Campos, M.G., Fratini, F., Altaye, S.Z., Li, J., and Qi, Y. (2020). New insights into the biological and pharmaceutical properties of royal jelly. Int. J. Mol. Sci., 21.10.3390/ijms21020382 ↗
5. Royal Jelly: An ancient remedy with remarkable antibacterial properties10.1016/j.micres.2016.06.007 ↗
6. Identification of freshness and metabolite changes of royal jelly during storage using Nano-ESI-MS and UPLC-Q/TOF-MS10.1016/j.jfca.2024.107092 ↗
7. Bărnuţiu, L.I., Mărghitaș, L.A., Dezmirean, D.S., Mihai, C.M., and Bobiș, O. (2011). Chemical composition and antimicrobial activity of Royal Jelly-REVIEW. Sci. Pap. Anim. Sci. Biotechnol., 44.
8. Kanelis, D., Liolios, V., Rodopoulou, M.A., Papadopoulou, F., and Tananaki, C. (2024). Production and Quality Characteristics of Royal Jelly in Relation to Available Natural Food Resources. Resources, 13.10.3390/resources13040055 ↗
9. Nutritional effects of some pollen types on hypopharyngeal and acid glands in honeybee workers (Apis mellifera L.)10.21608/jmr.2021.70288.1062 ↗
10. Influence of pollen quality on ovarian development in honeybee workers (Apis mellifera scutellata)10.1016/j.jinsphys.2007.04.002 ↗
11. Di Pasquale, G., Salignon, M., Le Conte, Y., Belzunces, L.P., Decourtye, A., Kretzschmar, A., Suchail, S., and Alaux, C. (2013). Influence of pollen nutrition on honeybee health: Do pollen quality and diversity matter?. PLoS ONE, 8.10.1371/journal.pone.0072016 ↗
12. Effects of pollen feeding on quality of royal jelly
13. Chan, Q.W., and Foster, L.J. (2008). Changes in protein expression during honey bee larval development. Genome Biol., 9, Available online: https://link.springer.com/article/10.1186/gb-2008-9-10-r156.10.1186/gb-2008-9-10-r156 ↗
14. Comparison of the nutrient composition of royal jelly and worker jelly of honey bees (Apis mellifera)10.1007/s13592-015-0374-x ↗
15. Collazo, N., Carpena, M., Nuñez-Estevez, B., Otero, P., Simal-Gandara, J., and Prieto, M.A. (2021). Health promoting properties of bee royal jelly: Food of the queens. Nutrients, 13.10.3390/nu13020543 ↗
16. Mantzourani, C., and Kokotou, M.G. (2023). Targeted and Suspect Fatty Acid Profiling of Royal Jelly by Liquid Chromatography—High Resolution Mass Spectrometry. Biomolecules, 13.10.3390/biom13030424 ↗
17. Antioxidant properties of royal jelly associated with larval age and time of harvest10.1021/jf802494e ↗
18. Botezan, S., Baci, G.M., Bagameri, L., Pașca, C., and Dezmirean, D.S. (2023). Current status of the bioactive properties of royal jelly: A comprehensive review with a focus on its anticancer, anti-inflammatory, and antioxidant effects. Molecules, 28.10.3390/molecules28031510 ↗
19. Milone, J.P., Chakrabarti, P., Sagili, R.R., and Tarpy, D.R. (2021). Colony-level pesticide exposure affects honey bee (Apis mellifera L.) royal jelly production and nutritional composition. Chemosphere, 263.10.1016/j.chemosphere.2020.128183 ↗
20. A review of sub-lethal neonicotinoid insecticides exposure and effects on pollinators10.1007/s40726-020-00142-8 ↗
21. Royal jelly production in Africanized honeybee colonies considering different protein supplements and the influence of environmental factors
22. Factors influencing the quality of Royal jelly and its components: A review10.1080/23311932.2024.2348253 ↗
23. A targeted approach for studying the effect of sugar bee feeding on the metabolic profile of Royal Jelly10.1016/j.chroma.2019.460783 ↗
24. Refractometric determination of water content in royal jelly10.1051/apido:2007053 ↗
25. Popescu, O., Mărghitaş, L.A., and Dezmirean, D. (2008). A study about physicochemical composition of fresh and lyophilized royal jelly. Sci. Pap. Anim. Sci. Biotechnol., 41.
26. Effect of supplementary honey and artificial sugar feeding of bees on the composition of royal jelly
27. Molecular insights into the enhanced performance of royal jelly secretion by a stock of honeybee (Apis mellifera ligustica) selected for increasing royal jelly production10.1007/s13592-019-00656-1 ↗
28. Standard methods for Apis mellifera royal jelly research10.1080/00218839.2017.1286003 ↗
29. Changes in composition of royal jelly harvested at different times: Consequences for quality standards10.1051/apido/2010033 ↗
30. Standard methods for artificial rearing of Apis mellifera larvae10.3896/IBRA.1.52.1.05 ↗
31. An introduction to high-yielding royal jelly production methods in China10.1080/0005772X.2002.11099543 ↗
32. A suggestion for royal jelly specifications
33. Physicochemical characterization of Persian fresh royal jelly samples10.1080/00218839.2023.2237726 ↗
34. Wang, Y., Ma, L., Wang, H., Liu, Z., Chi, X., and Xu, B. (2023). Effects of Sucrose Feeding on the Quality of Royal Jelly Produced by Honeybee Apis mellifera L. Insects 2023, 14, 742. Insects, 14.10.3390/insects14090742 ↗
35. Ghosh, S., Jang, H., Sun, S., and Jung, C. (2024). Nutrient Composition and Quality Assessment of Royal Jelly Samples Relative to Feed Supplements. Foods, 13.10.3390/foods13121942 ↗
36. Rearing Drones in Queen Cells of Apis mellifera Honey Bees
37. Lazaridou, E., Gounari, S., Xonis, K., and Aidinoglou, M. (2003, January 24–29). Factors affecting the production of royal jelly. Proceedings of the XXXVIIIth Apimondia International Apicultural Congress, Ljubjana, Slovenia.
38. Sidor, E., Miłek, M., Zaguła, G., Bocian, A., and Dżugan, M. (2021). Searching for differences in chemical composition and biological activity of crude drone brood and royal jelly useful for their authentication. Foods, 10.10.3390/foods10092233 ↗