journal article·2025-08-21Open access

Machine Learning-Based Prediction of Autism Spectrum Disorder and Discovery of Related Metagenomic Biomarkers with Explainable AI

Mustafa Temiz, Burcu Bakir-Gungor, Nur Ersoz, Malik Yousef

Applied Sciences· Volume 15· Issue 16

Abstract

Background: Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder characterized by social communication deficits and repetitive behaviors. Recent studies have suggested that gut microbiota may play a role in the pathophysiology of ASD. This study aims to develop a classification model for ASD diagnosis and to identify ASD-associated biomarkers by analyzing metagenomic data at the taxonomic level. Methods: The performances of five different methods were tested in this study. These methods are (i) SVM-RCE, (ii) RCE-IFE, (iii) microBiomeGSM, (iv) different feature selection methods, and (v) a union method. The last method is based on creating a union feature set consisting of the features with importance scores greater than 0.5, identified using the best-performing feature selection methods. Results: In our 10-fold Monte Carlo cross-validation experiments on ASD-associated metagenomic data, the most effective performance metric (an AUC of 0.99) was obtained using the union feature set (17 features) and the AdaBoost classifier. In other words, we achieve superior machine learning performance with a few features. Additionally, the SHAP method, which is an explainable artificial intelligence method, is applied to the union feature set, and Prevotella sp. 109 is identified as the most important microorganism for ASD development. Conclusions: These findings suggest that the proposed method may be a promising approach for uncovering microbial patterns associated with ASD and may inform future research in this area. This study should be regarded as exploratory, based on preliminary findings and hypothesis generation.
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Research context

OpenAlex topics: Autism Spectrum Disorder Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Management Information Systems, Faculty of Economics and Administrative Sciences, Sivas Cumhuriyet University, Sivas 58140, Türkiye

Department of Computer Engineering, Faculty of Engineering, Abdullah Gul University, Kayseri 38080, Türkiye

Department of Bioengineering, Graduate School of Engineering and Science, Abdullah Gul University, Kayseri 38080, Türkiye

Department of Information Systems, Zefat Academic College, Zefat 1320611, Israel

Galilee Digital Health Research Center (GDH), Zefat Academic College, Zefat 1320611, Israel

Funding

Abdullah Gul University Support Foundation · L’Oréal-UNESCO Young Women Scientist Program · Zefat Academic College · TUBITAK 2211-A BIDEB program

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