journal article·2025-04-11Open access

Elemental and Nutritional Characterisation with Vibrational Spectroscopy Analysis of Ulva sp., Gracilaria multipartita, and Sargassum muticum

Teresa Mouga, Mariana M. Almeida, Filipa Inês Pitacas, António Moitinho Rodrigues, Cláudia Vitória, Ofélia Anjos

Applied Sciences· Volume 15· Issue 8

Abstract

Macroalgae are rich in bioactive compounds with potential therapeutic applications, including antioxidant, antimicrobial, and nutritional properties. In this study, the biochemical composition of three seaweed species—Ulva sp., Gracilaria multipartita, and Sargassum muticum—was analysed, focusing on their mineral content and macronutrient profile. The ash, protein, fibre, lipid, and carbohydrate contents ranged from (dw) 26.56 to 33.53 g/100 g, 10.13 to 18.43 g/100 g, 0.11 to 0.19 g/100 g, 5.83 to 10.88 g/100 g, and 42.48 to 53.27 g/100 g, respectively. The findings indicate that the species studied are excellent sources of essential minerals, particularly magnesium, potassium, calcium, sodium, and trace elements, such as iron, zinc, copper, and manganese. Sargassum muticum exhibited the highest mineral content and thus holds significant promise for further exploration in biomedical and therapeutic contexts. FTIR, FT-RAMAN, and FT-NIR analysis highlight the presence of proteins, lipids, carbohydrates, including cell wall phycocolloids, and phenolic compounds. These results underscore the potential of macroalgae as natural sources of bioactive compounds for health-related applications, highlighting their value beyond traditional dietary supplementation.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Seaweed-derived Bioactive Compounds · Marine and coastal plant biology · Echinoderm biology and ecology

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

MARE—Marine and Environmental Sciences Center/ARNET-Aquatic Research Network, ESTM, Polytechnique University of Leiria, Edificio Cetmares, Av. Porto de Pesca, 2520-641 Peniche, Portugal

School Agriculture, Polytechnic University of Castelo Branco, 6000-909 Castelo Branco, Portugal

CERNAS-IPCB, Research Center for Natural Resources, Environment and Society, Polytechnic University of Castelo Branco, 6001-909 Castelo Branco, Portugal

CBP-BI, Centro de Biotecnologia de Plantas da Beira Interior, 6001-909 Castelo Branco, Portugal

Funding

FCT, Fundação para a Ciência e a Tecnologia

References (94)

Show source-provided references
1. The Potential of Seaweed Liquid Fertilizer on the Growth and Antioxidant Enhancement of Helianthus annuus L
2. Seaweed as Novel Food for Prevention and Therapy for Life Style Related Disease10.25220/WNJ.V05.i1.0001 ↗
3. Pereira, L. (2009). Guia Ilustrado Das Macroalgas: Conhecer e Reconhecer Algumas Espécies Da Flora Portuguesa, Imprensa da Universidade de Coimbra.10.14195/978-989-26-0397-1 ↗
4. The New Tree of Eukaryotes10.1016/j.tree.2019.08.008 ↗
5. Afonso, C., Correia, A.P., Freitas, M.V., Baptista, T., Neves, M., and Mouga, T. (2021). Seasonal Changes in the Nutritional Composition of Agarophyton vermiculophyllum (Rhodophyta, Gracilariales) from the Center of Portugal. Foods, 10.10.3390/foods10051145 ↗
6. Queirós, A.S., Circuncisão, A.R., Pereira, E., Válega, M., Abreu, M.H., Silva, A.M.S., and Cardoso, S.M. (2021). Valuable Nutrients from Ulva rigida: Modulation by Seasonal and Cultivation Factors. Appl. Sci., 11.10.3390/app11136137 ↗
7. McHugh, D.J. (2003). A Guide to the Seaweed Industry, Food and Agriculture Organisation of the United Nations. FAO Fisheries Technical Paper 441.
8. Assessment of the Nutritive, Biochemical, Antioxidant and Antibacterial Potential of Eight Tropical Macro Algae Along Kachchh Coast, India as Human Food Supplements10.1080/10498850.2017.1396274 ↗
9. Lomartire, S., and Gonçalves, A.M.M. (2022). An Overview of Potential Seaweed-Derived Bioactive Compounds for Pharmaceutical Applications. Mar. Drugs, 20.10.3390/md20020141 ↗
10. Biris-Dorhoi, E.S., Michiu, D., Pop, C.R., Rotar, A.M., Tofana, M., Pop, O.L., Socaci, S.A., and Farcas, A.C. (2020). Macroalgae—A Sustainable Source of Chemical Compounds with Biological Activities. Nutrients, 12.10.3390/nu12103085 ↗
11. Dietary Macrominerals: Updated Review of Their Role and Orchestration in Human Nutrition throughout the Life Cycle with Sex Differences10.1016/j.crfs.2023.100450 ↗
12. Seaweeds: A Sustainable Functional Food for Complementary and Alternative Therapy10.1016/j.tifs.2011.09.001 ↗
13. Guiry, M.D., and Guiry, G.M. (2025, January 22). AlgaeBase. World-Wide Electronic Publication. Available online: https://www.algaebase.org/.
14. Structural Development of Marine Green Alga (Ulva rigida C. Agardh, 1823) during Cultivation10.48048/tis.2023.6747 ↗
15. Womersley, H.B.S. (1984). The Marine Benthic Flora of Southern Australia Part I. Flora of Australia Supplementary Series, State Herbarium South Australia.
16. Freitas, M.V., Inácio, L.G., Martins, M., Afonso, C., Pereira, L., and Mouga, T. (2022). Primary Composition and Pigments of 11 Red Seaweed Species from the Center of Portugal. J. Mar. Sci. Eng., 10.10.3390/jmse10091168 ↗
17. Cotas, J., Leandro, A., Pacheco, D., Gonçalves, A.M.M., and Pereira, L. (2020). A Comprehensive Review of the Nutraceutical and Therapeutic Applications of Red Seaweeds (Rhodophyta). Life, 10.10.3390/life10030019 ↗
18. Samaraweera, A.M., Vidanarachchi, J.K., and Kurukulasuriya, M.S. (2011). Industrial Applications of Macroalgae. Handbook of Marine Macroalgae: Biotechnology and Applied Phycology, John Wiley & Sons.10.1002/9781119977087.ch33 ↗
19. World Bank Group (2016). Seaweed Aquaculture for Food Security, Income Generation and Environmental Health in Tropical Developing Countries, World Bank.
20. Gracilaria corallicola and G. multipartita (Gracilariales, Rhodophyta), Two Related Flattened European Species10.1080/09670262.2016.1201867 ↗
21. Variabilité Interspécifique de Trois Algues Rouges: Hypnea Musciformis, Gracilaria multipartita et Gelidium sesquipedale (Rhodophycées) de La Côte Atlantique Marocaine
22. Seasonal Variations of Growth and Agar Composition of Harvested along the Atlantic Coast of Morocco
23. Therapeutic Potential and Health Benefits of Sargassum species10.4103/0973-7847.125514 ↗
24. Global Seaweed Farming and Processing in the Past 20 Years10.1186/s43014-022-00103-2 ↗
25. High-Value Products from Macroalgae: The Potential Uses of the Invasive Brown Seaweed, Sargassum muticum10.1007/s11157-015-9381-7 ↗
26. Englen, A.H., Serebryakova, A., Ang, P., and Simmons, K.B. (2015). Climate-Driven Trophic Cascades Affecting Seabirds around the British Isles. Oceanography and Marine Biology, Oxford University Press.
27. Spotting Intruders: Species Distribution Models for Managing Invasive Intertidal Macroalgae10.1016/j.jenvman.2020.111861 ↗
28. Carbohydrate-Based Phenotyping of the Green Macroalga Ulva fasciata Using near-Infrared Spectrometry: Potential Implications for Marine Biorefinery10.1515/bot-2016-0039 ↗
29. Shining Light on Seaweed-the Utilization of Vibrational Spectroscopy and Machine Learning in the Seaweed Industry10.1093/ijfood/vvaf012 ↗
30. Complementarity of Raman and Infrared Spectroscopy for Rapid Characterization of Fucoidan Extracts10.1186/s13007-021-00830-6 ↗
31. Rapid Determination of Bulk Microalgal Biochemical Composition by Fourier-Transform Infrared Spectroscopy10.1016/j.biortech.2013.08.133 ↗
32. Cebi, N., Bekiroglu, H., and Erarslan, A. (2023). Nondestructive Metabolomic Fingerprinting: FTIR, NIR and Raman Spectroscopy in Food Screening. Molecules, 28.10.3390/molecules28237933 ↗
33. Vandanjon, L., Burlot, A.-S., Zamanileha, E.F., Douzenel, P., Ravelonandro, P.H., Bourgougnon, N., and Bedoux, G. (2023). The Use of FTIR Spectroscopy as a Tool for the Seasonal Variation Analysis and for the Quality Control of Polysaccharides from Seaweeds. Mar. Drugs, 21.10.3390/md21090482 ↗
34. Fourier Transform Infrared Spectroscopy of Microalgae as a Novel Tool for Biodiversity Studies, Species Identification, and the Assessment of Water Quality10.1111/j.1529-8817.2009.00662.x ↗
35. Feasibility of Spectroscopic Characterization of Algal Lipids: Chemometric Correlation of NIR and FTIR Spectra with Exogenous Lipids in Algal Biomass10.1007/s12155-010-9098-y ↗
36. AOAC (2016). Official Methods of Analysis, Association Official Analytical. [20th ed.].
37. Chemical Composition of Red, Brown and Green Macroalgae from Buarcos Bay in Central West Coast of Portugal10.1016/j.foodchem.2015.03.057 ↗
38. The Protein Content of Seaweeds: A Universal Nitrogen-to-Protein Conversion Factor of Five10.1007/s10811-015-0650-1 ↗
39. V Validation of the Test Method—Determination of Available Carbohydrates in Cereal and Cereal Products, Dairy Products, Vegetables, Fruit, and Related Food Products and Animal Feeds: Collaborative Study, Final Action 2020.0710.1093/jaoacint/qsac116 ↗
40. Characterization of Dietary Energy in Swine Feed and Feed Ingredients: A Review of Recent Research Results10.5713/ajas.14.0001R ↗
41. Shurson, G.C., Hung, Y.T., Jang, J.C., and Urriola, P.E. (2021). Measures Matter—Determining the True Nutri-Physiological Value of Feed Ingredients for Swine. Animals, 11.10.3390/ani11051259 ↗
42. (2011). Regulation (EU) No 1169/2011 of the European Parliament and of the Council on the provision of food information to consumers, of 25 October 2011. Of. J. Eur. Union, 304, 16–83.
43. Pedro, S.I., Fernandes, T.A., Luís, Â., Antunes, A.M.M., Gonçalves, J.C., Gominho, J., Gallardo, E., and Anjos, O. (2023). First Chemical Profile Analysis of Acacia Pods. Plants, 12.10.3390/plants12193486 ↗
44. Composition, Nutritional Aspects and Effect on Serum Parameters of Marine Algae Ulva rigida10.1002/jsfa.3836 ↗
45. Seasonal Variation in Phenolic Composition, Antibacterial and Antioxidant Activities of Ulva rigida (Chlorophyta) and Assessment of Antiacetylcholinesterase Potential10.1007/s10811-012-9866-5 ↗
46. Freitas, M.V., Mouga, T., Correia, A.P., Afonso, C., and Baptista, T. (2021). New Insights on the Sporulation, Germination, and Nutritional Profile of Gracilaria gracilis (Rhodophyta) Grown under Controlled Conditions. J. Mar. Sci. Eng., 9.10.3390/jmse9060562 ↗
47. Nutritional and Functional Bioactivity Value of Selected Azorean Macroalgae: Ulva Compressa, Ulva rigida, Gelidium microdon, and Pterocladiella capillacea10.1111/1750-3841.13778 ↗
48. Soares, C., Sousa, S., Machado, S., Vieira, E., Carvalho, A.P., Ramalhosa, M.J., Morais, S., Correia, M., Oliva-Teles, T., and Domingues, V.F. (2021). Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination. Foods, 10.10.3390/foods10061366 ↗
49. Fatty Acid Profiling of Tropical Marine Macroalgae: An Analysis from Chemotaxonomic and Nutritional Perspectives10.1016/j.phytochem.2012.10.015 ↗
50. Health-Promoting Ingredients from Four Selected Azorean Macroalgae10.1016/j.foodres.2016.08.007 ↗
51. Paiva, L.S. (2014). Development of Extraction and Quantification Technologies for the Main Nutritional Components of Macroalgae from the Azores Coast with a View to Their Utilisation as Food Supplements, Universidade dos Açores.
52. Nutritional Benefits of Edible Macroalgae from the Central Portuguese Coast: Inclusion of Low-Calorie ‘Sea Vegetables’ in Human Diet
53. Nutritional Value of Selected Macroalgae10.1007/s10811-010-9556-0 ↗
54. Salman, A., Ferreira, A., Soares, J., and Souza, J. (2010). Methodologies for Evaluating Feed for Domestic Ruminants, Embrapa. [1st ed.].
55. Extraction and Characterization of Protein from Irish Brown Seaweed Ascophyllum nodosum10.1016/j.foodres.2016.07.018 ↗
56. Seaweeds from the Portuguese Coast as a Source of Proteinaceous Material: Total and Free Amino Acid Composition Profile10.1016/j.foodchem.2018.06.145 ↗
57. Peñalver, R., Lorenzo, J.M., Ros, G., Amarowicz, R., Pateiro, M., and Nieto, G. (2020). Seaweeds as a Functional Ingredient for a Healthy Diet. Mar. Drugs, 18.10.3390/md18060301 ↗
58. Changes in Total Nitrogen and Amino Acid Composition of New Zealand Undaria Pinnatifida with Growth, Location and Plant Parts10.1016/j.foodchem.2014.06.016 ↗
59. Pangestuti, R., and Kim, S.K. (2015). Seaweed Proteins, Peptides, and Amino Acids. Seaweed Sustainability: Food and Non-Food Applications, Academic Press Inc.10.1016/B978-0-12-418697-2.00006-4 ↗
60. Mišurcová, L., Machů, L., and Orsavová, J. (2011). Seaweed Minerals as Nutraceuticals. Advances in Food and Nutrition Research, Academic Press Inc.10.1016/B978-0-12-387669-0.00029-6 ↗
61. World Health Organization (2012). Sodium Intake for Adults and Children, World Health Organization.
62. Circuncisão, A.R.S. (2017). Analysing the Nutritional Profile of Ulva rigida Cultivated under Different Conditions in an Integrated Multi-Trophic Aquaculture System, University of Aveiro.
63. Circuncisão, A.R., Catarino, M.D., Cardoso, S.M., and Silva, A.M.S. (2018). Minerals from Macroalgae Origin: Health Benefits and Risks for Consumers. Mar. Drugs, 16.10.3390/md16110400 ↗
64. Comparative Analysis of Sugar and Mineral Content of Sargassum Spp. Collected from Different Coasts of Sri Lanka10.1007/s10811-019-01770-4 ↗
65. Nutritional Composition and Bioactive Compounds of Red Seaweed: A Mini-Review10.12691/jfnr-8-8-7 ↗
66. Bioproducts from Seaweeds: A Review with Special Focus on the Iberian Peninsula10.2174/138527281807140515154116 ↗
67. Comparative Effects of a Novel Plant-Based Calcium Supplement with Two Common Calcium Salts on Proliferation and Mineralization in Human Osteoblast Cells10.1007/s11010-010-0402-0 ↗
68. Study of Mineral and Nutritional Composition of Some Seaweeds Found along the Coast of Gulf of Mannar, India
69. Nutritional Value of Edible Seaweeds10.1111/j.1753-4887.2007.tb00278.x ↗
70. Lomartire, S., Marques, J.C., and Gonçalves, A.M.M. (2021). An Overview to the Health Benefits of Seaweeds Consumption. Mar. Drugs, 19.10.3390/md19060341 ↗
71. Contreras-Porcia, L., Meynard, A., López-Cristoffanini, C., Latorre, N., and Kumar, M. (2017). Marine Metal Pollution and Effects on Seaweed Species. Systems Biology of Marine Ecosystems, Springer.10.1007/978-3-319-62094-7_3 ↗
72. Yates, A.A., Schlicker, S.A., and Suitor, C.W. (2001). Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc, National Academies Press.
73. Baghel, R.S., Choudhary, B., Pandey, S., Pathak, P.K., Patel, M.K., and Mishra, A. (2023). Rehashing Our Insight of Seaweeds as a Potential Source of Foods, Nutraceuticals, and Pharmaceuticals. Foods, 12.10.3390/foods12193642 ↗
74. Leandro, A., Pacheco, D., Cotas, J., Marques, J.C., Pereira, L., and Gonçalves, A.M.M. (2020). Seaweed’s Bioactive Candidate Compounds to Food Industry and Global Food Security. Life, 10.10.3390/life10080140 ↗
75. Seasonal and Geographical Variation in the Chemical Composition of the Red Alga Palmaria palmata (L.) Kuntze
76. Comprehensive Analysis of Dehydrated Edible Macroalgae: Volatile Compounds, Chemical Profiles, Biological Activities, and Cytotoxicity10.1007/s10811-024-03403-x ↗
77. Identification and Characterization of Marine Seaweeds for Biocompounds Production10.1016/j.eti.2021.101848 ↗
78. The Use of Near-Infrared and Mid-Infrared Spectroscopy to Rapidly Measure the Nutrient Composition and the in Vitro Rumen Dry Matter Digestibility of Brown Seaweeds10.1016/j.anifeedsci.2022.115239 ↗
79. Near-Infrared Spectroscopy and Hyperspectral Imaging: Non-Destructive Analysis of Biological Materials10.1039/C4CS00062E ↗
80. A Tutorial on near Infrared Spectroscopy and Its Calibration10.1080/10408347.2010.515468 ↗
81. Simpson, M.B. (2005). Near-Infrared Spectroscopy for Process Analytical Chemistry: Theory, Technology and Implementation. Process Analytical Technology, Blackwell Publishing.10.1002/9780470988459.ch3 ↗
82. Fatty Acid Composition, TLC Screening, ATR-FTIR Analysis, Anti-Cholinesterase Activity, and in Vitro Cytotoxicity to A549 Tumor Cell Line of Extracts of 3 Macroalgae Collected in Madeira10.1007/s10811-019-01884-9 ↗
83. Cell Surface Characterization of Some Oleaginous Green Algae10.1007/s10811-015-0768-1 ↗
84. Using FTIR Spectroscopy for Rapid Determination of Lipid Accumulation in Response to Nitrogen Limitation in Freshwater Microalgae10.1016/j.biortech.2010.01.065 ↗
85. Marine Algal Carbohydrates as Carbon Sources for the Production of Biochemicals and Biomaterials10.1016/j.biotechadv.2018.02.006 ↗
86. Identification of Selected Seaweed Polysaccharides (Phycocolloids) by Vibrational Spectroscopy (FTIR-ATR and FT-Raman)10.1016/j.foodhyd.2008.11.014 ↗
87. Rapid and Accurate Determination of Protein Content in North Atlantic Seaweed by NIR and FTIR Spectroscopies10.1016/j.foodchem.2022.134700 ↗
88. Querido, W., Kandel, S., and Pleshko, N. (2021). Applications of Vibrational Spectroscopy for Analysis of Connective Tissues. Molecules, 26.10.3390/molecules26040922 ↗
89. Michalak, I., Mironiuk, M., and Marycz, K. (2018). A Comprehensive Analysis of Biosorption of Metal Ions by Macroalgae Using ICP-OES, SEM-EDX and FTIR Techniques. PLoS ONE, 13.10.1371/journal.pone.0205590 ↗
90. FTIR Transmission Spectroscopy for Measurement of Algae Neutral Lipids
91. Characterization and Lead(II), Cadmium(II), Nickel(II) Biosorption of Dried Marine Brown Macro Algae Cystoseira barbata10.1007/s11356-012-0807-2 ↗
92. Carotenoids-Their Resonance Raman Spectra and How They Can Be Helpful in Characterizing a Number of Biological Systems
93. Rodrigues, D., Costa-Pinto, A.R., Sousa, S., Vasconcelos, M.W., Pintado, M.M., Pereira, L., Rocha-Santos, T.A.P., Da Costa, J.P., Silva, A.M.S., and Duarte, A.C. (2019). Sargassum Muticum and Osmundea Pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization. Mar. Drugs, 17.10.3390/md17040209 ↗
94. Ertani, A., Francioso, O., Tinti, A., Schiavon, M., Pizzeghello, D., and Nardi, S. (2018). Evaluation of Seaweed Extracts from Laminaria and Ascophyllum nodosum spp. As Biostimulants in Zea mays L. Using a Combination of Chemical, Biochemical and Morphological Approaches. Front. Plant Sci., 9.10.3389/fpls.2018.00428 ↗