Use of Black Pepper Essential Oil to Produce a Healthier Chicken Pâté
Source abstract
Abstract
This study aims to explore the effect of using black pepper essential oil (BPEO) to produce a healthier chicken pâté. Four different formulations were produced: a control formulation without back pepper and three with increasing BPEO contents. To test the effect of using BPEO, physiochemical analyses were performed at two different moments, 8 and 21 days after production. Microbiological analyses were performed 2, 9, 16, and 23 days after production. Sensory analysis to evaluate the pleasantness of the pâtés to consumers was performed 21 days after production. Finally, total phenol and flavonoid content and antioxidant activity were evaluated. Results show no significant physicochemical differences. Pâtés with no BPEO or black garlic were the most pleasant, but 0.3 or 0.5% of BPEO were not significantly less appreciated, while 1% of the EO caused a decrease in taste and global appreciation. The higher the BPEO content, the higher the phenol content, antioxidant (with an increase from 1.58 to 2.27 mg eq. Fe II/g of sample, in the Control at T23 and V3 at T23, respectively), and microbial activity (with total mesophiles count reduction from 5.91 to 5.21 log CFU/g sample in V3 from T9 to T16). The use of 1% of BPEO showed a significant effect on the reduction in mesophile counts for at least two weeks. These results highlight the potential for optimizing BPEO or black garlic content to ensure both consumer acceptance and enhanced functional properties. While further analysis will help pinpoint the best formulation, the current findings are a promising step towards achieving an optimal balance.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Piperaceae Chemical and Biological Studies · Olfactory and Sensory Function Studies · Essential Oils and Antimicrobial Activity
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
CIMO, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal
References (82)
Show source-provided references
1. Production of n-3 fatty acid enriched pork liver pâté10.1016/j.lwt.2003.11.006 ↗
2. A healthier oil combination and konjac gel as functional ingredients in low-fat pork liver pâté10.1016/j.meatsci.2010.12.028 ↗
3. Evaluation of protein structural changes and water mobility in chicken liver paste batters prepared with plant oil substituting pork back-fat combined with pre-emulsification10.1016/j.foodchem.2015.09.068 ↗
4. Skałecki, P., Kaliniak-Dziura, A., Domaradzki, P., Florek, M., Poleszak, E., and Dmoch, M. (2021). Effect of Pork Meat Replacement by Fish Products on Fatty Acid Content, Physicochemical, and Sensory Properties of Pork Pâtés. Appl. Sci., 11.10.3390/app11010188 ↗
5. Trends in functional beverages: Functional ingredients, processing tecnologies, stability, health benefits, and consumer perspective10.1016/j.foodres.2023.113046 ↗
6. FDA (2025, January 30). Essential Oils, Oleoresins (Solvent-Free), and Natural Extractives (Including Distillates). Substances Generally Recognized as Safe. Available online: https://www.law.cornell.edu/cfr/text/21/182.20.
7. Šojić, B., Milošević, S., Savanović, D., Zeković, Z., Tomović, V., and Pavlić, B. (2023). Isolation, Bioactive Potential, and Application of Essential Oils and Terpenoid-Rich Extracts as Effective Antioxidant and Antimicrobial Agents in Meat and Meat Products. Molecules, 28.10.3390/molecules28052293 ↗
8. The effect of coating incorporated with black pepper essential oil on the lipid deterioration and aroma quality of Jinhua ham10.1007/s11694-019-00195-4 ↗
9. Black pepper (Piper nigrum L.) essential oil counteracts dexamethasone-induced hepatic injury via modulating PGC-1α/ PPAR-α pathway in rats10.1016/j.arabjc.2024.105690 ↗
10. Zhang, J., Ye, K.P., Zhang, X., Pan, D.D., Sun, Y.Y., and Cao, J.X. (2017). Antibacterial Activity and Mechanism of Action of Black Pepper Essential Oil on Meat-Borne Escherichia coli. Front. Microbiol., 7.10.3389/fmicb.2016.02094 ↗
11. Mantzourani, I., Daoutidou, M., Dasenaki, M., Nikolaou, A., Alexopoulos, A., Terpou, A., Thomaidis, N., and Plessas, S. (2022). Plant extract and essential oil application against Food-Borne pathogens in raw pork meat. Foods, 11.10.3390/foods11060861 ↗
12. Effect of black pepper essential oil on the quality of fresh pork during storage10.1016/j.meatsci.2016.03.002 ↗
13. Najman, K., Król, K., and Sadowska, A. (2022). The Physicochemical Properties, Volatile Compounds and Taste Profile of BG (Allium sativum L.) Cloves, Paste and Powder. Appl. Sci., 12.10.3390/app12094215 ↗
14. Bioactive components of garlic and their physiological role in health maintenance: A review10.1016/j.fbio.2013.07.001 ↗
15. Shang, A., Cao, S.Y., Xu, X.Y., Gan, R.Y., Tang, G.Y., Corke, H., Mavumengwana, V., and Li, H.B. (2019). Bioactive compounds and biological functions of garlic (Allium sativum L.). Foods, 8.10.3390/foods8070246 ↗
16. (2002). Meat and Meat Products. Definition and Classification. In Portuguese Norm–Meat and Meat Products (Standard No. NP 588/2008).
17. AOAC International, and Cunniff, P. (1995). AOAC Official Methods of Analysis of AOAC International, The Association. [16th ed.].
18. Ph and Color
19. (2008). Determinação do pH (Método de referência). In Portuguese Norm–Meat and Meat Products (Standard No. NP-ISO-3441).
20. (2002). Determination of Moisture Content. Reference Method (ISO 1442: 1197). In Portuguese Norm–Meat and Meat Products (Standard No. NP 1614/2002).
21. (2002). Determination of Total Ashes. Reference Method. In Portuguese Norm–Meat and Meat Products (Standard No. NP-ISO-1615/2002).
22. (2002). Determination of Total Nitrogen Content. Reference Method (ISO 937: 1978). In Portuguese Norm–Meat and Meat Products (Standard No. NP 1612/2002).
23. (2002). Carnes e Produtos Cárneos
24. Determinação do teor em Hidroxiprolina. (Método de Referência). In Portuguese Norm-Meat and Meat Products (Standard No. NP 1987/2002).
25. The colour of cooked cured pork. I-Estimation of the nitric oxide-haem pigments10.1002/jsfa.2740070804 ↗
26. (1982). Meat and Meat Products—Determination of Chloride Content—Standard Method (Standard No. NP 1845/1982).
27. (2009). Determination of Thiobarbituric Acid (TBA) Levels, Spectrophotometer Method (Standard No. NP-ISO-3356/2009).
28. A simple method for isolation and purification of total lipids from animal tissues10.1016/S0021-9258(18)64849-5 ↗
29. The effect of cooking methods on nutritional value of foal meat10.1016/j.jfca.2015.04.007 ↗
30. Vieira, C., Sarmiento-García, A., García, J.J., Rubio, B., and Martínez, B. (2021). Quality and Shelf Life of Fresh Meat from Iberian Pigs as Affected by a New Form of Presentation of Oleic Acid and an Organic-Acid Mix in the Diet. Foods, 10.10.3390/foods10050985 ↗
31. Coronary heart disease: Seven dietary factors10.1016/0140-6736(91)91846-M ↗
32. Latimer, G.W. (2023). Detection and Confirmed Quantitation of Coliforms and E. coli in Foods: SimPlate Coliform and E. coli Color Indicator. Official Methods of Analysis of AOAC International, AOAC Publications. [22nd ed.].
33. Latimer, G.W. (2023). Motile Salmonella in All Foods: Immunodiffusion (1–2 TEST) Method. Official Methods of Analysis of AOAC International, AOAC Publications. [22nd ed.].
34. (2020). Microbiology of Food and Animal Feeding Stuffs—Horizontal Method for the Enumeration of Presumptive Bacillus cereus—Colony-Count Technique at 30 Degrees C (Standard No. ISO 7932:2004).
35. (2002). Microbiologia Alimentar: Regras Gerais Para a Contagem de Microrganismos: Contagem de Colónias a 30 C (Standard No. Norma Portuguesa NP 4405 2002). elab. CT 61 (DGFCQA).
36. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent10.1016/S0076-6879(99)99017-1 ↗
37. Analysis of propolis: Some parameters and procedures for chemical quality control10.1080/00218839.1998.11100961 ↗
38. Comparative evaluation of Fe(III) reducing power-based antioxidant capacity assays in the presence of phenanthroline, batho-phenanthroline, tripyridyltriazine (FRAP), and ferricyanide reagents10.1016/j.talanta.2007.01.019 ↗
39. How does the added fat source affect sensory quality of sheep and goat pâtés? Como a fonte de gordura adicionada afeta a qualidade sensorial de patês de ovinos e caprinos?10.1590/0103-8478cr20190029 ↗
40. Influence of electrochemically activated water on the physical properties and rheological indicators of meat pates10.15587/2706-5448.2023.278113 ↗
41. Barbora-Cánovas, G.V., Fontana, A.J., Schmidt, S.-J., and Labuza, T.P. (2020). Water Activity in Foods: Fundamentals and Aplications, John Wiley & Sons. [2nd ed.].10.1002/9781118765982 ↗
42. Effect of maltodextrin, sodium chloride, and liquid smoke on the mass transfer kinetics and storage stability of osmotically dehydrated beef meat
43. Changes on physico-chemical properties, lipid oxidation and volatile compounds during the manufacture of Celta dry-cured loin10.1007/s13197-014-1561-x ↗
44. Ferreira, I., Vasconcelos, L., Leite, A., Botella-Martínez, C., Pereira, E., Mateo, J., Kasaiyan, S., and Teixeira, A. (2022). Use of Olive and Sunflower Oil Hydrogel Emulsions as Pork Fat Replacers in Goat Meat Burgers: Fat Reduction and Effects in Lipidic Quality. Biomolecules, 12.10.3390/biom12101416 ↗
45. Physicochemical properties and nutritional composition of chicken patties with grey oyster mushroom stems10.33102/mjosht.v10i1.364 ↗
46. Innovative formulation in pâté using a gelled emulsión of hemp oil (Cannabis sativa L.) as fat replacer10.1016/j.lwt.2024.116630 ↗
47. Functional meat and vegetable pate with spirulina10.1088/1755-1315/845/1/012123 ↗
48. Glišić, M., Bošković Cabrol, M., Čobanović, N., Starčević, M., Samardžić, S., Veličković, I., and Maksimović, Z. (2024). The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés. Foods, 13.10.3390/foods13050788 ↗
49. Bogusz, R., Latoszewska, M., Szymańska, I., Jaworska, D., Szulc, K., Lipińska, E., Florowska, A., Nowacka, M., and Pietrzak, D. (2024). The Assessment of the Possibility of Using Yellow Mealworm Powder in Chicken and Pork Pâté Production. Appl. Sci., 14.10.3390/app14199038 ↗
50. Flavour perception of oxidation in beef10.1016/j.meatsci.2005.07.015 ↗
51. Leite, A., Vasconcelos, L., Ferreira, I., Domínguez, R., Pereira, E., Rodrigues, S., Lorenzo, J.M., and Teixeira, A. (2023). Effect of the Inclusion of Olive Cake in the Diet on the Physicochemical Characteristics of Dry-Cured Loin and Dry-Cured “Cachaço” of Bísaro Pig. Appl. Sci., 13.10.3390/app13031439 ↗
52. Influence of fat content on physico-chemical and oxidative stability of foal liver pâté10.1016/j.meatsci.2013.04.045 ↗
53. Effects of plant oil combinations substituting pork backfat combined with pre-emulsification on physicochemical, textural, microstructural and sensory properties of spreadable chicken liver pâté10.1111/jfq.12199 ↗
54. Biopolymeric emulsions with added silicon as pork fat substitutes in the reformulation of healthy pâtés: Effect on technological, nutritional and sensory properties and on lipid digestibility after in vitro gastrointestinal digestion10.1016/j.lwt.2024.116865 ↗
55. Almeida da Silva, B., de Castro, V.C.G., da Silva Araújo, C., Joele, M.R.S.P., Maciel e Silva, A.G., Rodrigues da Silva, J.A., da Silva, W.C., Rodrigues, E.C.N., and de Brito Lourenço-Júnior, J. (2024). Optimization of the process of preparation of liver pâtés and omental fat of lambs fed with lipid cakes. PLoS ONE, 19.10.1371/journal.pone.0304532 ↗
56. Reducing the sodium chloride content in chicken pate by using potassium and ammonium chloride10.1016/j.profoo.2015.09.006 ↗
57. Effect of fat content on physical, microbial, lipid and protein changes during chill storage of foal liver pâté10.1016/j.foodchem.2014.01.038 ↗
58. Nutritional and biological properties of extra virgin olive oil10.1021/jf103813t ↗
59. Influence of time of harvest and maturity index on olive oil yield and quality10.1016/j.scienta.2010.11.008 ↗
60. Oil fatty acid composition of eighteen Mediterranean olive varieties cultivated under the arid conditions of Boughrara (southern Tunisia)10.3989/gya.021109 ↗
61. Development characterization and stability of a white cachama pâté-type product (Piaractus brachypomus)10.1016/j.foodchem.2021.131660 ↗
62. Llauger, M., Guerrero, L., Arnau, J., Morera, A., Wakamatsu, J.I., Lorenzo, J.M., and Bou, R. (2024). Zinc Protoporphyrin-Rich Pork Liver Homogenates as Coloring Ingredients in Nitrite-Free Liver Pâtés. Foods, 13.10.20944/preprints202401.1292.v1 ↗
63. Evolutionary aspects of the dietary Omega-6:Omega-3 fatty acid ratio: Medical implications10.1159/000235706 ↗
64. Linoleic acid improves assembly of the CII subunit and CIII2/CIV complex of the mitochondrial oxidative phosphorylation system in heart failure. Cell Commun
65. Associations between Linoleic Acid Intake and Incident Type 2 Diabetes among U.S. Men and Women10.2337/dc19-0412 ↗
66. Effect of Weight, Sex and Hunting Period on Fatty Acid Composition of Intramuscular and Subcutaneous Fat from Wild Boar10.4081/ijas.2012.e32 ↗
67. Chen, J., and Liu, H. (2020). Nutritional Indices for Assessing Fatty acids: A Mini-Review. Int. J. Mol. Sci., 21.10.3390/ijms21165695 ↗
68. Physicochemical characteristics of three muscles from free-range reared Iberian pigs slaughtered at 90 kg live weight10.1016/S0309-1740(02)00180-8 ↗
69. Lyophilized and microencapsulated extracts of grape pomace from winemaking industry to prevent lipid oxidation in chicken pâté10.1590/1981-6723.11219 ↗
70. Essential oils as potential antimicrobial agents in meat and meat products: A review10.1016/j.tifs.2013.09.002 ↗
71. Food and Drug Administration (FDA) (2024, November 11). Food Code 2013. US Public Health Service, Available online: http://www.fda.gov/downloads/Food/GuidanceRegulation/RetailFoodProtection/FoodCode/UCM374510.pdf.
72. Leonard, W., Zhang, P., Ying, D., Adhikari, B., and Fang, Z. (2021). Fermentation transforms the phenolic profiles and bioactivities of plant-based foods. Biotechnol. Adv., 49.10.1016/j.biotechadv.2021.107763 ↗
73. Bucciantini, M., Leri, M., Nardiello, P., Casamenti, F., and Stefani, M. (2021). Olive Polyphenols: Antioxidant and Anti-Inflammatory Properties. Antioxidants, 10.10.3390/antiox10071044 ↗
74. Antibacterial plant compounds, extracts and essential oils: An updated review on their effects and putative mechanisms of action10.1016/j.phymed.2021.153626 ↗
75. Lorenzo, J.M., Domínguez, R., Pateiro, M., and Munekata, P.E.S. (2022). Dry-cured loin. Production of Traditional Mediterranean Meat Products, Humana. Methods and Protocols in Food Science.10.1007/978-1-0716-2103-5 ↗
76. Nutritional analysis and evaluation of the consumer acceptance of pork pâté enriched with cricket powder—Preliminary study10.1515/opag-2019-0015 ↗
77. Martins, A.J., Lorenzo, J.M., Franco, D., Pateiro, M., Domínguez, R., Munekata, P.E.S., Pastrana, L.M., Vicente, A.A., Cunha, R.L., and Cerqueira, M.A. (2020). Characterization of Enriched meat-based pâté manufactured with oleogels as fat substitutes. Gels, 6.10.3390/gels6020017 ↗
78. The influence of the substitution of fat with modified starch on the quality of pork liver pates10.1016/j.lwt.2020.110264 ↗
79. Antioxidant effects of BG powder on spent duck meat nugget quality during storage
80. Effect of aged garlic powder on physicochemical characteristics, texture profiles, and oxidative stability of ready-to-eat pork patties10.5713/ajas.18.0689 ↗
81. Augustynska-Prejsnar, A., Kačániová, M., Ormian, M., Topczewska, J., Sokołowicz, Z., and Hanus, P. (2024). Quality Assessment of Minced Poultry Products Including Black Fermented Garlic. Foods, 13.10.3390/foods13010070 ↗
82. The quality characteristics of sausage with added BG extracts10.9724/kfcs.2011.27.6.701 ↗