journal article·2024-03-05Open access

Gelatine-Based Biopolymer Film Produced from Ozone-Treated Film-Forming Solutions Containing Whey Protein Concentrate: Effects on Physical, Mechanical, and Thermal Characteristics

Emin Mercan

Applied Sciences· Volume 14· Issue 5

Abstract

This study aimed to determine the effects of the ozone treatment of film-forming solutions (FFSs) containing whey protein concentrate (WPC) and gelatine on biopolymer films’ physical, mechanical, and thermal properties. Film samples were produced from a FFS that was ozone-treated at 0 (control), 5, 10, and 30 min. Ozone treatment caused an increase in the pH values of the FFS, whereas the zeta potential remained negative. The films became lighter in colour, slightly greenish, and more opaque with longer ozonation times. The control sample showed the highest thermogravimetric weight loss (92.15%). The weight loss of the samples decreased with increasing ozone treatment time. The application of ozone treatment on the FFS enhanced the films’ mechanical properties. Increased ozone treatment time improved the film samples’ tensile strength, elongation at break, and toughness values. In conclusion, the results of this study demonstrate that the ozone treatment of FFS containing whey protein concentrate and gelatine can significantly enhance the physical, mechanical, and thermal properties of biopolymer films. These results highlight the potential of ozone treatment as a viable method for improving the performance and quality of biopolymer films used in food packaging, offering promising advantages for sustainable and environmentally friendly packaging solutions.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Nanocomposite Films for Food Packaging · biodegradable polymer synthesis and properties · Electrospun Nanofibers in Biomedical Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Food Engineering, Faculty of Engineering, Bayburt University, Bayburt 69000, Türkiye

References (61)

Show source-provided references
1. Shah, Y.A., Bhatia, S., Al-Harrasi, A., Afzaal, M., Saeed, F., Anwer, K., Khan, M.R., Jawad, M., Akram, N., and Faisal, Z. (2023). Mechanical Properties of Protein-Based Food Packaging Materials. Polymers, 15.10.3390/polym15071724 ↗
2. Fitriani, F., Aprilia, S., Arahman, N., Bilad, M.R., Suhaimi, H., and Huda, N. (2021). Properties of Biocomposite Film Based on Whey Protein Isolate Filled with Nanocrystalline Cellulose from Pineapple Crown Leaf. Polymers, 13.10.3390/polym13244278 ↗
3. Grafting Bacterial Cellulose Nanowhiskers into Whey Protein/Essential Oil Film Composites: Effect on Structure, Essential Oil Release and Antibacterial Properties of Films10.1016/j.foodhyd.2023.109374 ↗
4. Whey Protein Films Reinforced with Bacterial Cellulose Nanowhiskers: Improving Edible Film Properties Via a Circular Economy Approach10.1016/j.foodchem.2022.132604 ↗
5. Development of Gelatine-Based Edible Film by Addition of Whey Powders with Different Demineralisation Ratios: Physicochemical, Thermal, Mechanical and Microstructural Characteristics10.1111/1471-0307.12763 ↗
6. Herrera-Vázquez, S.E., Dublán-García, O., Arizmendi-Cotero, D., Gómez-Oliván, L.M., Islas-Flores, H., Hernández-Navarro, M.D., and Ramírez-Durán, N. (2022). Optimization of the Physical, Optical and Mechanical Properties of Composite Edible Films of Gelatin, Whey Protein and Chitosan. Molecules, 27.10.3390/molecules27030869 ↗
7. Effect of Whey Protein Purity and Glycerol Content Upon Physical Properties of Edible Films Manufactured Therefrom10.1016/j.foodhyd.2012.05.001 ↗
8. Effects of Ultraviolet Radiation on Properties of Films from Whey Protein Concentrate Treated before or after Film Formation10.1016/j.foodhyd.2015.11.019 ↗
9. Water Solubility, Mechanical, Barrier, and Thermal Properties of Cross-Linked Whey Protein Isolate-Based Films10.1111/j.1365-2621.2004.tb13365.x ↗
10. Properties of Transglutaminase Crosslinked Whey Protein Isolate Coatings and Cast Films10.1002/pts.2071 ↗
11. Γ-Irradiation Influence on the Structure and Properties of Calcium Caseinate−Whey Protein Isolate Based Films. Part 1. Radiation Effect on the Structure of Proteins Gels and Films10.1021/jf060486o ↗
12. Effects of Edible Coatings Based on Ultrasound-Treated Whey Proteins in Quality Attributes of Frozen Atlantic Salmon (Salmo Salar)10.1016/j.ifset.2011.12.003 ↗
13. Efficacy of Ozonation on Microbial Counts in Used Brines for Cheesemaking10.1016/j.idairyj.2015.06.003 ↗
14. Use of Ozone in the Dairy Industry: A Review10.1111/1471-0307.12302 ↗
15. Disinfection Effects and Operating Conditions of Tea Polyphenols Combined with Ozone10.1080/01919512.2020.1746632 ↗
16. Effects of Ozone Processing on Chemical, Structural and Functional Properties of Whey Protein Isolate10.1016/j.foodres.2014.10.002 ↗
17. Effects of Ozone on Functional Properties of Proteins10.1016/j.foodchem.2012.02.146 ↗
18. Biodegradable Films Produced from Ozone-Modified Potato Starch10.1007/s41783-020-00082-0 ↗
19. A New Ozonated Cassava Film with the Addition of Cellulose Nanofibres: Production and Characterization of Mechanical, Barrier and Functional Properties10.1007/s10924-020-02013-1 ↗
20. Ozonation of Cassava Starch to Produce Biodegradable Films10.1016/j.ijbiomac.2019.09.028 ↗
21. Scaling up Difficulties and Commercial Aspects of Edible Films for Food Packaging: A Review10.1016/j.tifs.2020.04.014 ↗
22. Advanced Technologies Applied to Enhance Properties and Structure of Films and Coatings: A Review10.1007/s11947-022-02768-6 ↗
23. A Comparative Study: Development and Characterization of Active Biodegradable Chicken Skin and Mammalian Gelatin Composite Films Incorporated with Curcumin Extracts10.1111/jfpp.15771 ↗
24. Preparation and Characterisation of Chicken Skin Gelatin as an Alternative to Mammalian Gelatin10.1016/j.foodhyd.2012.05.009 ↗
25. Physical, Structural, Antioxidant and Antimicrobial Properties of Gelatin–Chitosan Composite Edible Films10.1016/j.ijbiomac.2014.03.054 ↗
26. Işıl, V., and Sinan, U. (2019). Active Antimicrobial Food Packaging, IntechOpen.
27. On the Action of Ozone on Proteins10.1016/S0141-3910(03)00170-8 ↗
28. Ozone Degradation of Biological Macromolecules: Proteins, Hemoglobin, Rna, and DNA10.1080/01919510600900290 ↗
29. On the Action of Ozone on Gelatin10.1016/j.ijbiomac.2007.02.008 ↗
30. The Properties of Chitosan/Zein Blend Film and Effect of Film on Quality of Mushroom (Agaricus bisporus)10.1016/j.postharvbio.2019.05.013 ↗
31. Photocatalytic/Antimicrobial Active Film Based on Wheat Gluten/Zno Nanoparticles10.1007/s10904-019-01407-6 ↗
32. (2002). Standard Test Method for Tensile Properties of Thin Plastic Sheeting (Standard No. ASTM D882-02).
33. Effect of Ph and Lactose on Cross-Linking Extension and Structure of Fish Gelatin Films10.1016/j.reactfunctpolym.2017.04.005 ↗
34. Effect of Ph on Properties of Wheat Gluten and Soy Protein Isolate Films10.1021/jf00035a006 ↗
35. Surface, Mechanical and Barrier Properties of Bio-Based Composite Films Based on Chitosan and Whey Protein10.1016/j.fpsl.2014.01.001 ↗
36. Fine-Stranded and Particulate Gels of Β-Lactoglobulin and Whey Protein at Varying Ph10.1016/S0268-005X(09)80122-7 ↗
37. Viscoelastic Behaviour of Β-Lactoglobulin Gel Structures10.1016/S0268-005X(09)80013-1 ↗
38. Effect of High-Pressure Homogenization on Particle Size and Film Properties of Soy Protein Isolate10.1016/j.indcrop.2012.08.005 ↗
39. Effect of Heat Treatment on Physicochemical and Emulsifying Properties of Polymerized Whey Protein Concentrate and Polymerized Whey Protein Isolate10.1016/j.lwt.2018.08.028 ↗
40. Transparent and Edible Films from Ultrasound-Treated Egg Yolk Granules10.1007/s11947-017-2049-2 ↗
41. Nemati, V., Hashempour-Baltork, F., Gharavi-Nakhjavani, M.S., Feizollahi, E., Júnior, L.M., and Alizadeh, A.M. (2023). Application of a Whey Protein Edible Film Incorporated with Cumin Essential Oil in Cheese Preservation. Coatings, 13.10.3390/coatings13081470 ↗
42. Development and evaluation of a novel biodegradable film made from chitosan and cinnamon essential oil with low affinity toward water10.1016/j.foodchem.2010.02.033 ↗
43. Characterization of new biodegradable edible film made from basil seed (Ocimum basilicum L.) gum10.1016/j.carbpol.2013.10.062 ↗
44. Importance of surface tension characterization for food, pharmaceutical and packaging products: A review10.1080/10408390591000884 ↗
45. Fabrication of super-hydrophobic film from PMMA with intrinsic water contact angle below 9010.1016/j.polymer.2007.10.038 ↗
46. Gelatin-Based Nanocomposite Films: A Study on Montmorillonite Dispersion Methods and Concentration10.1016/j.jfoodeng.2014.11.009 ↗
47. Using Liquid Smoke to Improve Mechanical and Water Resistance Properties of Gelatin Films10.1111/1750-3841.13282 ↗
48. Kontogianni, V.G., Kosma, I., Mataragas, M., Pappa, E., Badeka, A.V., and Bosnea, L. (2023). Innovative Intelligent Cheese Packaging with Whey Protein-Based Edible Films Containing Spirulina. Sustainability, 15.10.3390/su151813909 ↗
49. The Impact of Ozone Treatment on Whey Concentrate on the Flow Behaviour, Functional and Microbiological Characteristics of Whey Powder10.1016/j.idairyj.2022.105447 ↗
50. The Effect of Ozone on Aflatoxin M1, Oxidative Stability, Carotenoid Content and the Microbial Count of Milk10.1080/01919512.2017.1329647 ↗
51. Physical, Mechanical, and Thermal Properties of Gelatine-Based Edible Film Made Using Kefir: Monitoring Aspergillus Flavus and A. Parasiticus Growth on the Film Surface
52. Effect of A-Tocopherol Antioxidant on Rheological and Physicochemical Properties of Chitosan/Zein Edible Films10.1016/j.lwt.2019.108799 ↗
53. Development of Disulfide Bond Crosslinked Gelatin/Ε-Polylysine Active Edible Film with Antibacterial and Antioxidant Activities10.1007/s11947-020-02420-1 ↗
54. A Gelatin-Based Film Reinforced by Covalent Interaction with Oxidized Guar Gum Containing Green Tea Extract as an Active Food Packaging System10.1007/s11947-020-02509-7 ↗
55. Films Based on Egg White Protein and Succinylated Casein Cross-Linked with Transglutaminase10.1007/s11947-017-1901-8 ↗
56. Whey Protein–Polysaccharide Blended Edible Film Formation and Barrier, Tensile, Thermal and Transparency Properties10.1002/jsfa.4502 ↗
57. Edible Films Based on Tapioca Starch and Wpc or Gelatine Plasticized with Glycerol: Potential Food Applications Based on Their Mechanical and Heat-Sealing Properties10.1007/s11947-023-03089-y ↗
58. Active Whey Protein Edible Films and Coatings Incorporating Lactobacillus Buchneri for Penicillium Nordicum Control in Cheese10.1007/s11947-020-02465-2 ↗
59. Water Vapor Permeability, Solubility, and Tensile Properties of Heat-Denatured Versus Native Whey Protein Films10.1111/j.1365-2621.1999.tb12276.x ↗
60. Composite Films of Regenerate Cellulose with Chitosan and Polyvinyl Alcohol: Evaluation of Water Adsorption, Mechanical and Optical Properties10.1016/j.ijbiomac.2018.05.148 ↗
61. Preparation and Incorporation of Functional Ingredients in Edible Films and Coatings10.1007/s11947-020-02528-4 ↗