The Loop-Mediated Isothermal Amplification for the Rapid Detection of Porphyromonas Gingivalis
Source abstract
Abstract
Periodontitis is a highly prevalent oral disease occurring across the globe, resulting from an interaction of many predisposing factors. Our study aimed to determine some chosen periopathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, Tannarella forsythia) using three different methods: loop-mediated isothermal amplification (LAMP), polymerase chain reaction (PCR) and real-time PCR (qPCR), and to compare their sensitivity and specificity. The study included 62 patients with diagnosed periodontitis. Gingival crevicular fluid was collected from all patients to take samples of bacterial DNA for the further laboratory molecular analyses. In comparison to the gold standard (qPCR), the best diagnostic quality parameters were achieved for LAMP using the TE buffer for the P. gingivalis determination. Therefore, the LAMP is an analytical technique that could be used to quickly assess the presence of periopathogens in an outpatient setting.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Oral microbiology and periodontitis research · Biosensors and Analytical Detection · Salivary Gland Disorders and Functions
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Conservative Dentistry and Endodontics, Poznan University of Medical Sciences, 60-812 Poznan, Poland
Student’s Scientific Group in Department of Conservative Dentistry and Endodontics, Poznan University of Medical Sciences, 60-812 Poznan, Poland
Department of Cancer Immunology, Poznan University of Medical Sciences, 61-866 Poznan, Poland
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