Polyphenol Content, Antiradical Properties, and α-Amylase Inhibition Activity of Vaccinium myrtillus L. (Bilberry) and Vaccinium vitis-idaea L. (Lingonberry) Leaf and Aerial Parts Extracts
Source abstract
Abstract
The utilisation of medicinal plants has gained importance due to emerging drug resistance in diseases, including type 2 diabetes mellitus (T2DM). Vaccinium myrtillus (VM) and Vaccinium vitis-idaea (VV) are particularly noteworthy as their leaves and aerial parts (no flowering leaves with stems) are rich in polyphenols and antioxidants with potential positive impacts on blood sugar levels. The aim of this study was to compare the chemical composition, antiradical, and α-amylase inhibitory activities of the leaves and aerial parts of the Latvian VM and VV. Two samples of VM and two samples of VV were collected from two different locations. Dried plants were used to prepare 70% ethanol extracts and freeze-dried samples. The total tannin and phenolic contents were determined, and the phytochemical compounds were characterised by LC-MS. The α-amylase inhibition activity and the antiradical activity in the extracts were measured. The VV ethanol extracts had the highest polyphenol content at 632.80 mg/100 g, followed by the freeze-dried extracts at 968.52 mg/100 g. The highest polyphenol content in the VV ethanol extracts was 632.80 mg/100 g and 968.52 mg/100 g in the freeze-dried extracts. The ethanol extracts of VM (1.34%) and VV (2.85%) had the highest content of tannin, whereas the freeze-dried extracts showed significantly lower tannin content in VM (0.21%) and VV (0.18%). A high correlation was observed between DPPH (2,2-diphenyl-1-picrylhydrazyl) and α-amylase inhibition activity (R = 0.86, p = 0.015). This information can be used for additional control of sugar levels in T2DM patients. Comparing both plants with each other and different types of samples (ethanol extracts, freeze-dried samples), it was concluded that the difference exists in both variants. The VM samples showed higher results (α-amylase inhibition activity, DPPH radical scavenging activity) than the VV samples, and the freeze-dried samples had higher results than the ethanol extracts.
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Research context
OpenAlex topics: Phytochemicals and Antioxidant Activities · Natural Antidiabetic Agents Studies · Natural product bioactivities and synthesis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Riga Stradiņš University, LV-1007 Riga, Latvia
Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, LV-1658 Riga, Latvia
Laboratory of Finished Dosage Forms, Riga Stradiņš University, LV-1007 Riga, Latvia
Department of Pharmaceuticals, Red Cross Medical College, Riga Stradiņš University, LV-1007 Riga, Latvia
Funding
European Union’s Horizon 2020 research and innovation program
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