journal article·2025-04-03Open access

Multifactorial Analysis of Wood Deterioration in Ancient Egypt: A Case Study of Khufu’s Second Solar Boat

Shimaa Ibrahim, Paola Grenni, Lucia Mancini, Marco Voltolini, Hanan Mohamed Kamal Abdel-Fatah, Ahmed Refaat, Dina M. Atwa

Applied Sciences· Volume 15· Issue 7

Abstract

This study presents a multi-analytical investigation of the wooden components from Khufu’s Second Solar Boat, one of the valuable archaeological discoveries in ancient Egypt. The research integrates advanced imaging and analytical techniques to identify wood species, assess deterioration patterns, and characterize the fungal and bacterial biodeteriogens. The initial visual examination documented the state of preservation at the time of discovery. Subsequently, the identification of the wood species was carried out. The deterioration status was assessed using a variety of tools, including scanning electron microscopy (SEM) and high-resolution synchrotron radiation computed microtomography (SR-μCT) for morphological alterations, X-ray diffraction (XRD) for crystallinity changes and Fourier-transform infrared spectroscopy (FTIR) to assess chemical degradation indexes of wood. Moreover, molecular techniques were used to identify and characterize the presence of biodeterioration agents. Results indicate that ancient craftsmen used cedar wood for the boat construction. The analysed samples exhibited advanced biotic and abiotic degradation, as evidenced by microbiological assessments, XRD measurements of cellulose crystallinity, FTIR indices, SEM micrographs and SR-μCT data. These results provide crucial insights into the long-term degradation processes of archaeological wood in arid environments, enhancing our understanding of ancient Egyptian woodworking practices and informing future conservation strategies for similar artifacts.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Cultural Heritage Materials Analysis · Archaeology and ancient environmental studies · Metallurgy and Cultural Artifacts

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Grand Egyptian Museum, Conservation Center, Ministry of Antiquities, Museums Sector, El-Remaya Square, Giza 12561, Egypt

National Research Council, Water Research Institute, Via Salaria km 29.300, Monterotondo, 00010 Rome, Italy

Department of Materials, Slovenian National Building and Civil Engineering Institute (ZAG), Dimičeva Ulica 12, 1000 Ljubljana, Slovenia

Department of Earth Sciences “Ardito Desio”, University of Milan, 20133 Milano, Italy

Department of Botany, Faculty of Science, Cairo University, Giza 12613, Egypt

Spectroscopy Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt

Molecular Modeling and Spectroscopy Laboratory, Centre of Excellence for Advanced Science, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt

Department of Laser Interaction with Matters, Laser Institute for Research and Applications, Beni-Suef University, Beni-Suef 62517, Egypt

Funding

International Atomic Energy Agency (IAEA) · International Centre for Theoretical Physics (ICTP)

References (100+)

Show source-provided references
1. Nicholson, P.T., and Shaw, I. (2000). Wood. Ancient Egyptian Materials and Technology, Cambridge University Press.
2. Kim, Y., and Singh, A.P. (2016). Wood as Cultural Heritage Material and its Deterioration by Biotic and Abiotic Agents. Secondary Xylem Biology, Elsevier.10.1016/B978-0-12-802185-9.00012-7 ↗
3. Jenkins, N. (1980). The Boat Beneath The Pyramid. King Cheops’ Royal Ship, Holt Rinehart & Winston.
4. The Abydos BG 10 Boat and Implications for Standardisation, Innovation, and Timber Conservation in Early Dynastic Boat-Building10.1177/030751331209800107 ↗
5. Moustafa, A.Y., Osman, M.S., Nour, M.Z., and Iskander, Z. (1960). The Cheops Boats.
6. Preliminary Stability and Resistance Analysis of the Cheops Boat10.5957/jspd.2020.36.1.14 ↗
7. Landström, B. (1970). Ships of the Pharaohs; 4000 Years of Egyptian Shipbuilding, Doubleday.
8. Wendrich, W., Dieleman, J., Frood, E., and Baines, J. (2013). Transportation. UCLA Encyclopedia of Egyptology, University of California.
9. Van de Moortel, A. (2000). Sacred and Secular: Ancient Egyptian Ships and Boats, University of Pennsylvania Museum.
10. Ship Timber and the Reuse of Wood in Ancient Egypt10.1163/18741665-12340007 ↗
11. Bui, H. (2012). Density Images by Microgravity. Imaging the Cheops Pyramid. Solid Mechanics and Its Applications, Springer.10.1007/978-94-007-2657-4 ↗
12. Yoshimura, K. (2024). A Comparative Study Between Khufu’s First and Second Boats in Respect of Their Materials, Archaeological Conditions, and Conservation. [Master’s Thesis, American University in Cairo, AUC Knowledge Fountain].
13. Evaluating the impact of water flotation and the state of the wood in archaeological wood charcoal remains: Implications for the reconstruction of past vegetation and identification of firewood gathering strategies at Tell Qarassa North (south Syria)10.1016/j.quaint.2016.06.030 ↗
14. Conservation of A Painted Wooden Coffin at Dahshur Archaeological Area10.36868/IJCS.2024.01.01 ↗
15. Analytical Instrumental Techniques to Study Archaeological Wood Degradation10.1080/05704928.2015.1046181 ↗
16. Atwa, D.M., Ibrahim, S., Stani, C., Birarda, G., Ali, N., Abdullah, E., Vaccari, L., Grenni, P., Visca, A., and Badr, Y. (2022). Biodeterioration Assessment of a Unique Old Pharaonic Kingdom Wooden Statue Using Advanced Diagnostic Techniques. Appl. Sci., 12.10.3390/app12147020 ↗
17. Physicochemical structure and micromechanical properties of archaeological wood under alternating dry and wet conditions10.1080/17480272.2023.2286628 ↗
18. Szwajca, A., Lucejko, J.J., Berdychowska, N., and Zborowska, M. (2024). Understanding changes in holocellulose and lignin compounds in wooden structure of veneers: Molecular insights post hydrothermal treatment and aging. Int. J. Biol. Macromol., 266.10.1016/j.ijbiomac.2024.130920 ↗
19. Han, L., Xi, G., Dai, W., Zhou, Q., Sun, S., Han, X., and Guo, H. (2023). Influence of Natural Aging on the Moisture Sorption Behaviour of Wooden Structural Components. Molecules, 28.10.3390/molecules28041946 ↗
20. Microbiome Diversity and Cellulose Decomposition Processes by Microorganisms on the Ancient Wooden Seawall of Qiantang River of Hangzhou, China10.1007/s00248-023-02221-x ↗
21. Wang, H., Feyereisen, G.W., Zhang, J., and Ishii, S. (2025). Fungal degradation of complex organic carbon supports denitrification in saturated woodchip bioreactors. Bioresour. Technol., 417.10.1016/j.biortech.2024.131826 ↗
22. Stelzner, I., Stelzner, J., Gwerder, D., Martinez-Garcia, J., and Schuetz, P. (2023). Imaging and Assessment of the Microstructure of Conserved Archaeological Pine. Forests, 14.10.3390/f14020211 ↗
23. Bouramdane, Y., Haddad, M., Mazar, A., Aît Lyazidi, S., Oudghiri Hassani, H., and Boukir, A. (2024). Aged Lignocellulose Fibers of Cedar Wood (9th and 12th Century): Structural Investigation Using FTIR-Deconvolution Spectroscopy, X-Ray Diffraction (XRD), Crystallinity Indices, and Morphological SEM Analyses. Polymers, 16.10.3390/polym16233334 ↗
24. Abdel-Azeem, A.M., Held, B.W., Richards, J.E., Davis, S.L., and Blanchette, R.A. (2019). Assessment of biodegradation in ancient archaeological wood from the Middle Cemetery at Abydos, Egypt. PLoS ONE, 14.10.1371/journal.pone.0213753 ↗
25. Assessment of Deterioration in Archaeological Wood from Ancient Egypt10.1179/019713694806066428 ↗
26. Investigating the effects of humidity and salt crystallisation on medieval masonry10.1016/S0360-1323(00)00015-9 ↗
27. Critical evaluation of global mechanisms of wood devolatilization10.1016/j.tca.2005.02.030 ↗
28. New data on relevant ancient Egyptian wooden artifacts: Identification of wooden species and study of the state of conservation with multidisciplinary analyses10.1111/arcm.12815 ↗
29. Yang, W., Ma, W., and Liu, X. (2024). Evaluation of Deterioration Degree of Archaeological Wood from Luoyang Canal No. 1 Ancient Ship. Forests, 15.10.3390/f15060963 ↗
30. New insights into a polychrome Middle Kingdom palette applied to a wooden coffin: A multidisciplinary analytical approach10.1016/j.culher.2022.01.015 ↗
31. Multi-analytical approach for the study of an ancient Egyptian wooden statuette from the collection of Museo Egizio of Torino10.21014/acta_imeko.v11i1.1089 ↗
32. Examination and analysis of a stored stucco window in the conservation lab of Bab Al-azab area, citadel of Salah Al-din Al-Ayyubi, Cairo Egypt10.1016/j.radphyschem.2024.111627 ↗
33. Balzano, A., Merela, M., and Čufar, K. (2022). Scanning Electron Microscopy Protocol for Studying Anatomy of Highly Degraded Waterlogged Archaeological Wood. Forests, 13.10.3390/f13020161 ↗
34. A critical evaluation of the degradation state of dry archaeological wood from Egypt by SEM, ATR-FTIR, wet chemical analysis and Py(HMDS)-GC-MS10.1016/j.polymdegradstab.2017.10.009 ↗
35. Application of FTIR spectroscopy to the characterization of archeological wood10.1016/j.saa.2015.07.108 ↗
36. Afifi, H.A.M., Mansour, M.M.A., Hassan, A.G.A.I., and Salem, M.Z.M. (2023). Biodeterioration effects of three Aspergillus species on stucco supported on a wooden panel modeled from Sultan al-Ashraf Qaytbay Mausoleum, Egypt. Sci. Rep., 13.10.1038/s41598-023-42028-x ↗
37. Geweely, N.S., Abu Taleb, A.M., Grenni, P., Caneva, G., Atwa, D.M., Plaisier, J.R., and Ibrahim, S. (2024). Eco-Friendly Preservation of Pharaonic Wooden Artifacts using Natural Green Products. Appl. Sci., 14.10.3390/app14125023 ↗
38. Ancient basketry on the inside: X-ray computed microtomography for the non-destructive assessment of small archaeological monocotyledonous fragments: Examples from Southeast Europe10.1186/s40494-021-00631-z ↗
39. X-ray Computed Tomography for the anatomical and dendrochronological analysis of archaeological wood10.1016/j.jas.2014.12.015 ↗
40. Comparison of different experimental approaches in the tomographic analysis of ancient violins10.1016/j.culher.2017.02.013 ↗
41. Synchrotron Radiation Microtomography: A Tool for the Non-Invasive Structural Analysis of Historical Musical Instruments10.1080/08940886.2019.1680210 ↗
42. Synchrotron radiation microtomography of musical instruments: A non-destructive monitoring technique for insect infestations10.4081/jear.2011.149 ↗
43. X-ray computed microtomography and the identification of wood taxa selected for archaeological artefact manufacture: Rare examples from Australian contexts
44. Assessment of microbiological contamination in the work environments of museums, archives and libraries10.1007/s10453-015-9372-8 ↗
45. Yoon, J.H., Park, J.E., Suh, D.Y., Hong, S.B., Ko, S.J., and Kim, S.H. (2007). Comparison of Dyes for Easy Detection of Extracellular Cellulases in Fungi. Mycobiology, 35.10.4489/MYCO.2007.35.1.021 ↗
46. Preservation status and microbial community of waterlogged archaeological woods over 7800 years old at the Jingtoushan Site, China10.1007/s00226-023-01463-9 ↗
47. Abdrabou, A., Zidan, E., Nishisaka, A., Kurokochi, H., and Yoshimura, S. (2022). King Khufu’s Second Boat: Scientific Identification of Wood Species for Deckhouse, Canopy, and Forecastle. Forests, 13.10.3390/f13122118 ↗
48. Scientific Species Identification for the Deckhouse, Canopy and Forecastle of King Khufu’s Second Boat
49. Wood identification of some coffins from the Necropolis of Thebes held in the collection of the Egyptian Museum in Florence10.1016/j.culher.2020.09.007 ↗
50. SYRMEP beamline: State of the art, upgrades and future prospects10.1140/epjp/s13360-024-05489-1 ↗
51. Observation of microstructure and damage in materials by phase sensitive radiography and tomography10.1063/1.364374 ↗
52. Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object10.1046/j.1365-2818.2002.01010.x ↗
53. Enhanced and Flexible Software Tools for X-ray Computed Tomography at the Italian Synchrotron Radiation Facility Elettra10.3233/FI-2015-1273 ↗
54. NIH Image to ImageJ: 25 years of image analysis10.1038/nmeth.2089 ↗
55. Long, K., Chen, K., Lin, L., Fu, F., and Zhong, Y. (2023). Deterioration of Microstructures and Properties in Ancient Architectural Wood from Yingxian Wooden Pagoda (1056 AD) during Natural Aging. Forests, 14.10.3390/f14020393 ↗
56. MCX: A Synchrotron Radiation Beamline for X-ray Diffraction Line Profile Analysis10.1002/zaac.201400163 ↗
57. The X-ray diffraction beamline MCX at Elettra: A case study of non-destructive analysis on stained glass10.21014/acta_imeko.v6i3.464 ↗
58. An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer10.1177/004051755902901003 ↗
59. Classification of Lignins from Different Botanical Origins by FT-IR Spectroscopy10.1515/hfsg.1991.45.s1.21 ↗
60. FTIR studies of the changes in wood chemistry following decay by brown-rot and white-rot fungi10.1016/S0964-8305(03)00052-0 ↗
61. The Effect of Fungal Decay on Ficus Sycomorus Wood
62. Comparative study of historical woods from XIX century by thermogravimetry coupled with FTIR spectroscopy10.1007/s10570-019-02688-3 ↗
63. Crystallinity of wood and the size of cellulose crystallites in Norway spruce (Picea abies)10.1007/s10086-003-0518-x ↗
64. Deterioration of the cell wall in waterlogged wooden archeological artifacts, 2400 years old10.1163/22941932-40190241 ↗
65. Development of a Method for Identifying Wood Species in Archaeological Materials by IR Spectroscopy10.1134/S1061934819120104 ↗
66. Oak wood degradation processes induced by the burial environment in the archaeological site of Biskupin (Poland)10.1186/s40494-020-00390-3 ↗
67. Characterization of cellulose crystallinity after enzymatic treatment using Fourier transform infrared spectroscopy (FTIR)10.1007/s10570-017-1542-0 ↗
68. Raper, K.B., Thom, C., and Fennell, D.I. (1949). A Manual of the Penicillia, Williams & Wilkins.
69. Raper, K., and Fennell, D. (1977). The Genus Aspergillus, Krieger Publishing Company, Huntington.
70. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.010.1093/molbev/mst197 ↗
71. Raper, K., and Fennell, D. (1965). The Genus Aspergillus, Williams and Wilkins Company.
72. Gilman, J.C. (1977). A Manual of Soil Fungi, Biotech Books.
73. The Genus Penicillium and Its Teleomorphic States Eupenicillium and Talaromyces10.2307/3759616 ↗
74. Soil fungi in Qatar and other Arab countries10.1007/BF02861455 ↗
75. Kern, M.E., and Blevins, K.S. (1997). Laboratory procedures for fungal culture and isolation. Medical Mycology: A Self-Instructional Text, FA Davis Company.
76. Domsch, K.H., Gams, W., and Anderson, T.H. (2007). Compendium of Soil Fungi, Academic Press. [2nd ed.].
77. Klich, M.A. (2002). Identification of Common Aspergillus Species, Centraal Bureau Voor Schim.
78. Fungi as deterioration agents of historic glass plate negatives of Brandys family collection10.1016/j.ibiod.2016.08.002 ↗
79. White, T.J., Bruns, T., Lee, S., and Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols, Elsevier.10.1016/B978-0-12-372180-8.50042-1 ↗
80. Anatomy of Lebanon cedar (Cedrus libani A. Rich.) wood with indented growth rings
81. Arbuckle MacLeod, C. (2024). Lebanese Cedar, Skeuomorphs, Coffins, and Status in Ancient Egypt. Arts, 13.10.3390/arts13060163 ↗
82. Wilkinson, T. (2005). The Thames & Hudson Dictionary of Ancient Egypt, Thames & Hudson.
83. Broda, M., Popescu, C.-M., Curling, S.F., Timpu, D.I., and Ormondroyd, G.A. (2022). Effects of Biological and Chemical Degradation on the Properties of Scots Pine Wood—Part I: Chemical Composition and Microstructure of the Cell Wall. Materials, 15.10.3390/ma15072348 ↗
84. Detection and quantification of pore, solid and gravel spaces in CT images of a 3D soil sample10.1016/j.apm.2020.04.021 ↗
85. Bouramdane, Y., Fellak, S., El Mansouri, F., and Boukir, A. (2022). Impact of Natural Degradation on the Aged Lignocellulose Fibers of Moroccan Cedar Softwood: Structural Elucidation by Infrared Spectroscopy (ATR-FTIR) and X-ray Diffraction (XRD). Fermentation, 8.10.3390/fermentation8120698 ↗
86. Analysis of the biocorrosion community from ancient wooden constructions at Tianluoshan (7000–6300 cal BP), Zhejiang Province, China10.1186/s40494-024-01304-3 ↗
87. Methionine oxidation of carbohydrate-active enzymes during white-rot wood decay10.1128/aem.01931-23 ↗
88. Zalamea, M., González, G., and Lodge, D. (2016). Physical, Chemical, and Biological Properties of Soil under Decaying Wood in a Tropical Wet Forest in Puerto Rico. Forests, 7.10.3390/f7080168 ↗
89. Calcite deposition in karst waters is promoted by leaf litter breakdown and vice versa10.1051/limn/2010023 ↗
90. Rate of occurrence of wood degradation in foundations and archaeological sites when groundwater levels are too low10.1016/j.culher.2023.07.005 ↗
91. Overview of the geomorphological and hydrogeological characteristics of the Eastern Desert of Egypt10.1007/s10040-004-0364-y ↗
92. Geological and topographical study of the original hills at the base of Fourth Dynasty Egyptian monuments of the Memphite plateau10.2113/gssgfbull.181.3.279 ↗
93. Degradation of Lime Wood Painting Supports II. Spectral Characterisation
94. Degradation Phenomena of Wooden Pillars in the Main Hall of the Fengguo Monastery, Yixian, Liaoning, China—Scientific Investigation with XRD, IC, and FTIR Analysis10.12654/JCS.2020.36.1.02 ↗
95. Evaluation of degradation in chemical compounds of wood in historical buildings using FT-IR and FT-Raman vibrational spectroscopy
96. FTIR spectroscopy of woods: A new approach to study the weathering of the carving face of a sculpture10.1016/j.saa.2014.10.011 ↗
97. Infrared and Raman spectra of lignin substructures: Coniferyl alcohol, abietin, and coniferyl aldehyde10.1002/jrs.5588 ↗
98. Distinction of four Dalbergia species by FTIR, 2 nd derivative IR, and 2D-IR spectroscopy of their ethanol-benzene extractives10.1515/hf-2015-0125 ↗
99. Differentiating between Natural and Modified Cellulosic Fibres Using ATR-FTIR Spectroscopy10.3390/heritage5040213 ↗
100. FTIR-ATR-based prediction and modelling of lignin and energy contents reveals independent intra-specific variation of these traits in bioenergy poplars10.1186/1746-4811-7-9 ↗