journal article·2025-01-26Open access

Validating Accelerated Shelf Life Testing Methodology for Predicting Shelf Life in High-Pressure-Processed Meat Products

Athina Ntzimani, Maria Tsevdou, Evangelos Andrianos, Dimitrios Gounaris, Theodosios Spiliotopoulos, Petros Taoukis, Maria C. Giannakourou

Applied Sciences· Volume 15· Issue 3

Abstract

The shelf life of meat products is a critical factor in ensuring both consumer safety and product quality. Traditional methods for determining shelf life are labor-intensive and time-consuming, making it challenging for manufacturers to adapt to market demands. The accelerated shelf life testing (ASLT) methodology offers a viable solution by exposing products to controlled elevated conditions that simulate long-term storage, allowing for faster shelf life predictions. This study evaluates the ASLT methodology as a predictive tool for determining the shelf life of high-pressure (HPP)-treated meat products. The present study includes experiments to verify the shelf life of frankfurter-type sausages under accelerated conditions. By simulating long-term storage at elevated temperatures (4–18 °C), a kinetic model was developed to monitor spoilage bacteria growth, with validation under dynamic temperature conditions. The results indicate that the main spoilage population of frankfurter-type sausages was lactic acid bacteria (LAB), which was strongly correlated with the total mesophilic microflora of the products. The findings show that elevated storage temperatures (8 and 18 °C) provide accurate shelf life predictions, offering an efficient approach to ensure product quality and meet consumer demands.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Meat and Animal Product Quality

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Laboratory of Food Chemistry and Technology, School of Chemical Engineering, National Technical University of Athens, 5 Heroon Polytechniou Str., 157 80 Athens, Greece

Research and Development Department, P.G. NIKAS S.A., 22nd km Athens-Lamia National Road, 145 65 Agios Stefanos, Attica, Greece

References (63)

Show source-provided references
1. Domínguez, R., Gómez, M., Fonseca, S., and Lorenzo, J.M. (2020). Effect of different preservative strategies on the shelf-life of fresh meat products. Appl. Sci., 10.
2. Natural antioxidants used in meat products: A brief review10.1016/j.meatsci.2018.10.016 ↗
3. Antioxidant effects of Juniperus communis L. essential oil in cooked pork sausages
4. Consumers’ perceptions of intrinsic and extrinsic attributes as indicators of safety and quality of chicken meat: Actionable information for public health authorities and the chicken industry10.1111/1750-3841.15125 ↗
5. Novel processing technologies and ingredient strategies for the reduction of phosphate additives in processed meat10.1016/j.tifs.2019.10.001 ↗
6. Control of Listeria monocytogenes by applying ethanol-based antimicrobial edible films on ham slices and microwave-reheated frankfurters10.1016/j.fm.2015.10.013 ↗
7. Lipid and protein oxidation and sensory properties of vacuum-packaged dry-cured ham subjected to high hydrostatic pressure10.1016/j.meatsci.2010.02.024 ↗
8. Argyri, A.A., Tassou, C.C., Samaras, F., Mallidis, C., and Chorianopoulos, N. (2014). Effect of high hydrostatic pressure processing on microbiological shelf-life and quality of fruits pretreated with ascorbic acid or SnCl2. BioMed Res. Int., 2014.10.1155/2014/819209 ↗
9. Katsaros, G., and Taoukis, P. (2021). Microbial control by high pressure processing for shelf-life extension of packed meat products in the cold chain: Modeling and case studies. Appl. Sci., 11.10.3390/app11031317 ↗
10. High-pressure treatment of dry-cured Iberian ham. Effect on colour and oxidative stability during chill storage packed in modified atmosphere10.1007/s00217-005-0176-x ↗
11. Volatile compounds in fresh meats subjected to high pressure processing: Effect of the packaging material10.1016/j.meatsci.2008.08.008 ↗
12. New insights into the high-pressure processing of meat and meat products10.1111/j.1541-4337.2012.00184.x ↗
13. Fennema, O. (1996). Summary: Integrative Concepts (Shelf-life testing and modelling). Food Chemistry, Marcel Dekker. [3rd ed.]. Chapter 17.
14. Valentas, K.J., Rotstein, E., and Singh, R.P. (1997). Kinetics of food deterioration and shelf-life prediction. The Handbook of Food Engineering Practice, CRC Press. Chapter 10.10.1201/9780367802110 ↗
15. Kilcast, D., and Subramaniam, P. (2011). Accelerated shelf life testing of foods. Food and Beverage Stability and Shelf Life, Woodhead Publishing. Chapter 15.10.1533/9780857092540 ↗
16. Steele, R. (2004). Temperature and food stability: Analysis and control. Understanding and Measuring the Shelf-Life of Food, Technology and Nutrition. Chapter 3.
17. Predicting the shelf life of cup chocolate using the Arrhenius model based on peroxide value10.18280/mmep.110224 ↗
18. Comparative effects of HPP and irradiation on plant-based whole hard-boiled eggs10.59796/jcst.V14N3.2024.66 ↗
19. Effect of selected starter cultures on the microbiological, physicochemical, and sensory attributes of Greek dry-fermented sausages
20. Critical quality constraints of sensory attributes in frankfurter-type sausages, to be applied in optimization models10.1016/j.lwt.2004.08.004 ↗
21. Meat spoilage during distribution10.1016/j.meatsci.2007.06.020 ↗
22. Shelf-life prediction models for ready-to-eat fresh cut salads: Testing in real cold chain10.1016/j.ijfoodmicro.2016.09.032 ↗
23. Sensitive spectrophotometric method for lipid hydroperoxide determination10.1007/BF02635914 ↗
24. Speck, M.L. (1984). APHA Technical Committee on Microbiological Methods for Foods, American Public Health Association.
25. (2023). Microbiology of the Food Chain—Horizontal Method for the Enumeration of Coagulase-Positive staphylococci (Staphylococcus aureus and Other Species)—Part 2: Method Using Rabbit Plasma Fibrinogen Agar Medium. Amendment 1 (Standard No. ISO 6888-2:2021/Amd 1:2023).
26. (2017). Microbiology of the Food Chain—Horizontal Method for the Detection and Enumeration of Enterobacteriaceae. Part 2: Colony-Count Technique. Published (Edition 2, 2017) (Standard No. ISO 21528-2:2017).
27. (2003). Microbiology of Food and Animal Feeding Stuffs—Protocol for the Validation of Alternative Methods (Standard No. ISO 16140:2003).
28. EFSA BIOHAZ Panel (EFSA Panel on Biological Hazards), Koutsoumanis, K., Alvarez-Ordonez, A., Bolton, D., Bover-Cid, S., Chemaly, M., Davies, R., De Cesare, A., Herman, L., and Hilbert, F. (2022). The efficacy and safety of high-pressure processing of food. EFSA J., 20, 7128–7323.10.2903/j.efsa.2022.7128 ↗
29. High-pressure processing of meat: Molecular impacts and industrial applications10.1111/1541-4337.12670 ↗
30. A dynamic approach to predicting bacterial growth in food10.1016/0168-1605(94)90157-0 ↗
31. (2006). Sensory Analysis—General Guidance for the Staff of a Sensory Evaluation Laboratory—Part 1: Staff Responsibilities (Standard No. ISO 13300-1).
32. (2006). Sensory Analysis—General Guidance for the Staff of a Sensory Evaluation Laboratory—Part 2: Recruitment and Training of Panel Leaders (Standard No. ISO 13300-2).
33. (2017). Sensory Analysis—Methodology—General Guidance (Standard No. ISO 6658).
34. (2019). Sensory Analysis—General Guidance for the Application of Sensory Analysis in Quality Control (Standard No. ISO 20613).
35. Effect of orange dietary fibre, oregano essential oil and packaging conditions on shelf-life of bologna sausages10.1016/j.foodcont.2009.07.004 ↗
36. The function and mechanism of lactic acid bacteria in the reduction of toxic substances in food: A review10.1080/10408398.2021.1895059 ↗
37. Heterolactic fermentation by a homofermentative Lactobacillus sp. during glucose limitation in anaerobic continuous culture with complete cell recycle10.1111/j.1365-2672.1991.tb04457.x ↗
38. Evaluation of shelf life of cured, cooked, sliced turkey fillets and cooked pork sausages-‘piroski’- Stored under vacuum and modified atmospheres at+ 4 and+ 10° C10.1016/S0309-1740(01)00224-8 ↗
39. Effect of high pressure treatment on microbial populations of sliced vacuum-packed cooked ham10.1016/j.meatsci.2011.02.029 ↗
40. Xiao, Q., Xu, M., Xu, B., Chen, C., Deng, J., and Li, P. (2021). Combined effect of high-pressure processing with spice extracts on quality of low-salt sausage during refrigerated storage. Foods, 10.10.3390/foods10112610 ↗
41. High pressure processing applied to cooked sausage manufacture: Physical properties and sensory analysis10.1016/S0309-1740(03)00013-5 ↗
42. Packaging alternatives to deliver fresh meat using short- or long-term distribution10.1016/S0963-9969(01)00096-5 ↗
43. Lipid and protein oxidation in charqui meat and jerked beef10.1590/S1516-89132013000100014 ↗
44. Protein oxidation in frankfurters with increasing levels of added rosemary essential oil: Effect on colour and texture deterioration
45. Shelf-life extension of vacuum-packed salt reduced frankfurters and cooked ham through the combined application of high pressure processing and organic acids10.1016/j.fpsl.2018.06.008 ↗
46. Jensen, C., Skibsted, L.H., and Bertelsen, G. (1998). Oxidative stability of chilled meats. Antioxidants in Muscle Foods, Springer.
47. Effects of ascorbic acid and antioxidants on color, lipid oxidation, and volatiles of irradiated ground beef
48. Modified atmosphere packaging and vacuum packaging for long period chilled storage of dry-cured Iberian ham10.1016/j.meatsci.2009.11.013 ↗
49. Development of frankfurter-type sausages with healthy lipid formulation and their nutritional, sensory and stability properties10.1002/ejlt.201400157 ↗
50. High pressure treatment of dry-cured Iberian ham. Effect on radical formation, lipid oxidation and colour10.1007/s00217-004-0939-9 ↗
51. Effects of high pressure processing on lipid oxidation: A review10.1016/j.ifset.2013.10.012 ↗
52. Shelf-life prediction of chicken nuggets based on the thickness level of composite plastic packaging using accelerated shelf-life testing (ASLT) method by the Arrhenius equation approach10.1088/1755-1315/1297/1/012078 ↗
53. Influence of HPP conditions on selected beef quality attributes and their stability during chilled storage10.1016/j.meatsci.2010.10.022 ↗
54. Spoilage microbiota associated with the storage of raw meat in different conditions10.1016/j.ijfoodmicro.2012.05.020 ↗
55. Bacterial spoilers of food: Behavior, fitness, and functional properties10.1016/j.fm.2014.03.009 ↗
56. The effect of modified atmosphere and vacuum packaging on the spoilage microorganisms of beef meat
57. High-pressure processing of meat, meat products and seafood10.1007/s12393-010-9028-y ↗
58. Zhu, Y., Li, X., Da, C., Liang, P., Jin, S., and Tang, C. (2022). Effects of Cyclocarya paliurus (Batal.) extracts on oxidative stability and sensory quality in meat products (Frankfurters). Foods, 11.10.3390/foods11223721 ↗
59. Shelf-life extension of cooked ham model product by high hydrostatic pressure and natural preservatives10.1016/j.ifset.2011.07.009 ↗
60. Efficiency of high hydrostatic pressure at 600 MPa against food-borne microorganisms by challenge tests on convenience meat products10.1016/j.lwt.2008.12.001 ↗
61. Effects of high hydrostatic pressure and varying concentrations of sodium nitrite from traditional vegetable-based sources on the growth of Listeria monocytogenes on ready-to-eat (RTE) sliced ham
62. Effects of high pressure treatment on the quality of cooked pork ham prepared with different levels of curing ingredients10.1080/08957950601091087 ↗
63. Effects of high-pressure processing technique on the quality and shelf life of Chinese style sausages