journal article·2025-01-16Open access

Comprehensive Evaluation of the Nutritional Quality of Stored Watermelon Seed Oils

Marta Siol, Beata Witkowska, Diana Mańko-Jurkowska, Sina Makouie, Joanna Bryś

Applied Sciences· Volume 15· Issue 2

Abstract

The appropriate use of food industry by-products such as watermelon seeds could reduce the problem of food waste, following the “zero waste” concept. Research in recent years suggests that these unused waste products could be a source of nutrients and bioactive compounds. Accordingly, the present study aimed to evaluate the nutritional potential and selected quality parameters of watermelon seed oils. Four commercial oils (three unrefined cold-pressed and one refined pressed) and one self-extracted oil were considered. The oils were analyzed over three months of storage after opening/extraction to determine their fatty acid (FA) composition and distribution, hydrolytic and oxidative stability, and selected health indices. Linoleic acid was the predominant FA, ranging from 52.9% (refined oil) to 62.2% (self-extracted oil). Refined oil demonstrated superior oxidative stability, with the lowest acid value (AV) and peroxide value (PV) throughout the storage period, adhering to the Codex Alimentarius standards. Unrefined oils, particularly WO3, showed significantly higher AVs and PVs after storage, indicating greater susceptibility to hydrolytic and oxidative changes. Health indices were favorable for all oils, with self-extracted oil exhibiting the highest health-promoting index (7.07) and hypocholesterolemic/hypercholesterolemic ratio (7.18). Oxidative stability showed that self-extracted oil had significantly higher stability (76.6 min) than other tested oils, despite having the highest PUFA content. In turn, refinement has a significant effect on the AVs and PVs and the oxidative stability of oil, achieving the lowest PUFA level (53.61%). These results emphasize the potential of watermelon seed oil as a health-promoting product and emphasize the role of production and storage conditions in maintaining its quality.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Advances in Cucurbitaceae Research · Phytochemicals and Antioxidant Activities · Seed and Plant Biochemistry

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, 02-776 Warsaw, Poland

Faculty of Food Technology, Warsaw University of Life Sciences, 02-776 Warsaw, Poland

References (61)

Show source-provided references
1. Antioxidant, Antibacterial Properties and the Light Barrier Assessment of Raw and Purified Melanins Isolated from Citrullus lanatus (Watermelon) Seeds10.2478/hepo-2018-0008 ↗
2. Recovery of High Value-Added Compounds from Pineapple, Melon, Watermelon and Pumpkin Processing by-Products: An Overview10.1016/j.foodres.2020.109086 ↗
3. (2023). FAOSTAT Statistical Database, Food and Agriculture Organization of the United Nations.
4. (2024, September 18). Zero Waste International Allince. Available online: https://zwia.org/zero-waste-definition.
5. Bioactive Compounds and Antioxidant Activities of Different Watermelon (Citrullus lanatus (Thunb.) Mansfeld) Cultivars as Affected by Fruit Sampling Area10.1016/j.jfca.2010.06.005 ↗
6. Utilization of Watermelon Rinds and Sharlyn Melon Peels as a Natural Source of Dietary Fiber and Antioxidants in Cake10.1016/j.aoas.2013.01.012 ↗
7. Utilization of Watermelon Peel as a Pectin Source and the Effect of Ultrasound Treatment on Pectin Film Properties10.1016/j.lwt.2021.111569 ↗
8. Phytochemical and Antibacterial Properties of the Seed of Watermelon (Citrullus lanatus)
9. Phytochemicals and Antioxidants in Watermelon (Citrullus lanatus) Genotypes under Hot Arid Region10.56093/ijas.v85i3.47179 ↗
10. Effects of Methanolic Extract of Citrullus lanatus Seed on Experimentally Induced Prostatic Hyperplasia10.9734/EJMP/2011/588 ↗
11. In Vitro Antimicrobial Activities of Chloroformic, Hexane and Ethanolic Extracts of Citrullus lanatus var. Citroides (Wild Melon)
12. Antidiabetic Activity and Chemical Composition of Sanabi Melon Seed Oil10.1155/2018/5434156 ↗
13. Hepatoprotective Effects of Aqueous Extract of Watermelon (Citrullus lanatus) Seeds on Ethanol-Induced Oxidative Damage in Wister Rats10.4103/ssajm.ssajm_18_18 ↗
14. Characterization and Functional Properties of Watermelon (Citrullus lanatus) Seed Protein Isolates and Salt Assisted Protein Concentrates10.1007/s10068-011-0122-6 ↗
15. Evaluation of the Medicinal Properties and Possible Nutrient Composition of Citrullus lanatus (Watermelon) Seeds10.3923/rjmp.2019.129.135 ↗
16. A Review on Food Values of Selected Tropical Fruits’ Seeds10.1080/10942912.2014.980946 ↗
17. Chemistry and Technology of Melon Seeds
18. Nutritional Assessment of Defatted Oil Meals of Melon (Colocynthis citrullus L.) and Flutes Pumpkin (Telfaria occidentalis Hook) by Chick Assay10.1002/jsfa.2740380307 ↗
19. Characteristics and Composition of Melon Seed Oil
20. Characterization of Two Moroccan Watermelon Seeds Oil Varieties by Three Different Extraction Methods10.1051/ocl/2020010 ↗
21. Influence of Moisture Content and Temperature on Mechanical Extraction of Oil from Watermelon (Citrullus lanatus) Seeds
22. Use of GC and PDSC Methods to Characterize Human Milk Fat Substitutes Obtained from Lard and Milk Thistle Oil Mixtures10.1007/s10973-017-6452-8 ↗
23. (2001). Vegetable and Animal Oils and Fats. Preparation of Fatty Acid Methyl Esters. (Standard No. PN-EN ISO 5509:2001).
24. Chen, J., and Liu, H. (2020). Nutritional Indices for Assessing Fatty Acids: A Mini-Review. Int. J. Mol. Sci., 21.10.3390/ijms21165695 ↗
25. Wirkowska-Wojdyła, M., Ostrowska-Ligęza, E., Górska, A., Brzezińska, R., and Piasecka, I. (2024). Assessment of the Nutritional Potential and Resistance to Oxidation of Sea Buckthorn and Rosehip Oils. Appl. Sci., 14.10.3390/app14051867 ↗
26. Effect of Genotype, Feeding System and Slaughter Weight on the Quality of Light Lambs: II. Fatty Acid Composition of Meat10.1016/S0301-6226(02)00059-3 ↗
27. Bandara, R.R., Louis-Gavet, C., Bryś, J., Mańko-Jurkowska, D., Górska, A., Brzezińska, R., Siol, M., Makouie, S., Palani, B.K., and Obranović, M. (2024). Enzymatic Interesterification of Coconut and Hemp Oil Mixtures to Obtain Modified Structured Lipids. Foods, 13.10.3390/foods13172722 ↗
28. (2009). Acid Value Official Methods and Recommended Practices of the AOCS (Standard No. AOCS Official Method Te 1a-64).
29. Firestone, D. (2009). AOCS Official Method Cd 8b-90, AOCS Press. Peroxide Value Acetic Acid-Isooctane Method Official Methods and Recommended Practices of the AOCS, 6th ed.
30. Siol, M., Dudek, A., Bryś, J., Mańko-Jurkowska, D., Gruczyńska-Sękowska, E., Makouie, S., Palani, B.K., Obranović, M., and Koczoń, P. (2024). Chromatographic and Thermal Characteristics, and Hydrolytic and Oxidative Stability of Commercial Pomegranate Seed Oil. Foods, 13.10.3390/foods13091370 ↗
31. Study on Effect of Destroyed Seeds Content on the Quality of Cold Pressed Rapeseed Oil
32. Fatty Acid Composition and Oxidative Characteristics of Novel Edible Oils in Poland10.1080/19476337.2016.1190406 ↗
33. Fatty Acid Profile of Milk from Saanen and Swedish Landrace Goats10.1016/j.foodchem.2018.02.041 ↗
34. Coronary heart disease: Seven dietary factors10.1016/0140-6736(91)91846-M ↗
35. Phytochemical Content, Oxidative Stability, and Nutritional Properties of Unconventional Cold-Pressed Edible Oils10.12691/jfnr-6-7-9 ↗
36. Górska, A., Piasecka, I., Wirkowska-Wojdyła, M., Bryś, J., Kienc, K., Brzezińska, R., and Ostrowska-Ligęza, E. (2023). Berry Seeds—A By-Product of the Fruit Industry as a Source of Oils with Beneficial Nutritional Characteristics. Appl. Sci., 13.10.3390/app13085114 ↗
37. Ratusz, K., Symoniuk, E., Wroniak, M., and Rudzińska, M. (2018). Bioactive Compounds, Nutritional Quality and Oxidative Stability of Cold-Pressed Camelina (Camelina sativa L.) Oils. Appl. Sci., 8.10.3390/app8122606 ↗
38. Triacylglycerols in Edible Oils: Determination, Characterization, Quantitation, Chemometric Approach and Evaluation of Adulterations10.1016/j.chroma.2017.08.002 ↗
39. The relations between minor components and antioxidant capacity of five fruits and vegetables seed oils in China10.5650/jos.ess19005 ↗
40. Montesano, D., Blasi, F., Simonetti, M., Santini, A., and Cossignani, L. (2018). Chemical and Nutritional Characterization of Seed Oil from Cucurbita maxima L. (var. Berrettina) Pumpkin. Foods, 7.10.3390/foods7030030 ↗
41. (2009). Codex Alimentarius 2009. Codex Standard for Named Vegetable Oils. (Standard No. Codex-ALINORM 09/32/17).
42. Induced Changes in Olive Oil Subjected to Various Chemical Refining Steps: A Comparative Study of Quality Indices, Fatty Acids, Bioactive Minor Components, and Oxidation Stability Kinetic Parameters
43. Quality Assessment of Selected Refined and Cold-Pressed Rapeseed Oils Available on Polish Market
44. Seed Storability in Three Varieties of Papaya in Relation to Seed Moisture, Packaging Material and Storage Temperature10.15258/sst.2008.36.3.21 ↗
45. Quality Parameters for Cold Pressed Edible Argan Oils
46. Symoniuk, E., Wroniak, M., Napiórkowska, K., Brzezińska, R., and Ratusz, K. (2022). Oxidative Stability and Antioxidant Activity of Selected Cold-Pressed Oils and Oils Mixtures. Foods, 11.10.3390/foods11111597 ↗
47. Cheikhyoussef, N., Kandawa-Schulz, M., Böck, R., de Koning, C., Cheikhyoussef, A., and Hussein, A.A. (2017). Characterization of Acanthosicyos horridus and Citrullus lanatus Seed Oils: Two Melon Seed Oils from Namibia Used in Food and Cosmetics Applications. 3 Biotech, 7.10.1007/s13205-017-0922-3 ↗
48. Watermelon Seed Oil: Its Extraction, Analytical Studies, Modification and Utilization in Cosmetic Industries
49. Some Physicochemical Properties of the Petroleum Ether-Extracted Watermelon (Citrullus lanatus) Seed Oil10.3923/ajsr.2015.519.525 ↗
50. Production of Biodiesel from Watermelon (Citrullus lanatus) Seed Oil
51. Vegetable Oils Oxidation: Mechanisms, Consequences and Protective Strategies10.1080/87559129.2022.2026378 ↗
52. Domínguez, R., Pateiro, M., Gagaoua, M., Barba, F.J., Zhang, W., and Lorenzo, J.M. (2019). A Comprehensive Review on Lipid Oxidation in Meat and Meat Products. Antioxidants, 8.10.3390/antiox8100429 ↗
53. (2017). Vegetable and Animal Oils and Fats. Determination of Peroxide Number (Reference Method). (Standard No. PN-EN ISO 3960:2017-03).
54. Chemical Investigation of Nigella sativa L. Seed Oil Produced in Morocco
55. Functional and Physicochemical Properties of Watermelon (Citrullus lanatus) Seed and Seed-Oil10.9790/5736-0222931 ↗
56. Variety of Watermelon and Method of Drying Affect the Chemical and Functional Characteristics of Oils Extracted from Watermelon Seeds10.22259/2637-5583.0302004 ↗
57. Górska, A., Mańko-Jurkowska, D., Bryś, J., and Górska, A. (2021). Lipid Fraction Properties of Homemade Raw Cat Foods and Selected Commercial Cat Foods. Appl. Sci., 11.10.3390/app112210905 ↗
58. Oxidative Stability, Thermal Decomposition, and Oxidation Onset Prediction of Carrot, Flax, Hemp, and Canola Seed Oils in Relation to Oil Composition and Positional Distribution of Fatty Acids10.1002/ejlt.201500208 ↗
59. The Influence of Triacylglycerol Structure on the Oxidative Stability of Polyunsaturated Oils10.1002/lite.200800051 ↗
60. Piasecka, I., Górska, A., Ostrowska-Ligęza, E., and Kalisz, S. (2021). The Study of Thermal Properties of Blackberry, Chokeberry and Raspberry Seeds and Oils. Appl. Sci., 11.10.3390/app11167704 ↗
61. Impact of Selected Chemical Characteristics of Cold-Pressed Oils on their Oxidative Stability Determined Using the Rancimat and Pressure Differential Scanning Calorimetry Method10.1007/s12161-017-1081-1 ↗