journal article·2025-05-26Open access

Identification of Factors Influencing Fluoride Content in Tea Infusions: A Systematic Review

Agata Małyszek, Sylwia Kiryk, Julia Kensy, Agnieszka Kotela, Mateusz Michalak, Jan Kiryk, Maciej Janeczek, Jacek Matys, Maciej Dobrzyński

Applied Sciences· Volume 15· Issue 11

Abstract

Tea is one of the most widely consumed beverages globally and a significant dietary source of fluoride. This systematic review aimed to identify and evaluate the factors influencing fluoride concentration in tea infusions. A comprehensive literature search was conducted in March 2025 across PubMed, Scopus, and Web of Science databases, following PRISMA guidelines (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines and the PICO framework. Eligible studies investigated fluoride release in tea infusions, published in English from the year 2000 onward. Thirty articles met the inclusion criteria, and the risk of bias in the articles was assessed using the Joanna Briggs Institute (JBI) quality checklist. Fluoride concentration in tea infusions varied widely across studies, ranging from 0.008 to over 8 mg/L. Key factors influencing fluoride release included tea type (with black and green teas showing the highest values), leaf form (powdered and bagged teas released more fluoride than loose leaves), brewing time and temperature, water composition, and the presence of additives such as spices. A longer brewing time and higher temperature consistently increased fluoride extraction. Lower pH or water hardness also significantly affected fluoride availability. Regional origin of tea and production methods were additional sources of variation. Fluoride release in tea is influenced by a complex interplay of botanical, environmental, and preparation-related factors. These findings are clinically relevant, particularly for populations at risk of fluoride overexposure. Further standardized research is needed to inform safe consumption guidelines and public health recommendations.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Fluoride Effects and Removal

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biostructure and Animal Physiology, Wrocław University of Environmental and Life Sciences, Kożuchowska 1, 51-631 Wroclaw, Poland

Department of Pediatric Dentistry and Preclinical Dentistry, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland

Faculty of Dentistry, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland

Medical Center of Innovation, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland

Dental Surgery Department, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland

Funding

a subsidy from Wroclaw Medical University

References (74)

Show source-provided references
1. Fluoride Contamination, Consequences and Removal Techniques in Water: A Review10.1039/D1VA00039J ↗
2. The Fluoride Debate: The Pros and Cons of Fluoridation10.3746/pnf.2018.23.3.171 ↗
3. Lubojanski, A., Piesiak-Panczyszyn, D., Zakrzewski, W., Dobrzynski, W., Szymonowicz, M., Rybak, Z., Mielan, B., Wiglusz, R.J., Watras, A., and Dobrzynski, M. (2023). The Safety of Fluoride Compounds and Their Effect on the Human Body—A Narrative Review. Materials, 16.10.3390/ma16031242 ↗
4. Water Fluoridation: A Critical Review of the Physiological Effects of Ingested Fluoride as a Public Health Intervention10.1155/2014/293019 ↗
5. Fluoride agents in the prevention and treatment of dental caries and erosion in children, adolescents and adults—Recommendations of Polish Experts. Update of recommendations: Individual fluoride prevention in children and adolescents—Recommendations of Polish Experts
6. Assessment of the influence of vegetarian diet on the occurrence of erosive and abrasive cavities in hard tooth tissues10.5604/17322693.967066 ↗
8. Nassar, Y., and Brizuela, M. (2025). The Role of Fluoride on Caries Prevention. StatPearls, StatPearls Publishing.
9. Role of Fluoride in Dentistry: A Narrative Review
10. Piszko, P.J., Piszko, A., Kiryk, S., Kiryk, J., Kensy, J., Michalak, M., Matys, J., and Dobrzyński, M. (2025). Fluoride Release from Two Commercially Available Dental Fluoride Gels—In Vitro Study. Gels, 11.10.3390/gels11020135 ↗
11. Jullien, S. (2021). Prophylaxis of Caries with Fluoride for Children under Five Years. BMC Pediatr., 21.10.1186/s12887-021-02702-3 ↗
12. Fluoride and Its Implications on Oral Health: A Review10.4103/jpbs.jpbs_929_23 ↗
13. Toxicity of Fluoride: Critical Evaluation of Evidence for Human Developmental Neurotoxicity in Epidemiological Studies, Animal Experiments and in Vitro Analyses10.1007/s00204-020-02725-2 ↗
14. Assessment of Fluoride Concentration and Daily Intake by Human from Tea and Herbal Infusions10.1016/j.fct.2007.10.039 ↗
15. Waugh, D.T., Potter, W., Limeback, H., and Godfrey, M. (2016). Risk Assessment of Fluoride Intake from Tea in the Republic of Ireland and Its Implications for Public Health and Water Fluoridation. Int. J. Environ. Res. Public. Health, 13.10.3390/ijerph13030259 ↗
16. Fluoride content of solid foods impacts daily intake10.1111/j.1752-7325.2011.00292.x ↗
17. Analysis of Naturally Occurring Fluoride in Commercial Teas and Estimation of Its Daily Intake through Tea Consumption10.1111/1750-3841.13180 ↗
18. Comparison of Fluoride Ion Concentration in Black, Green and White Tea10.47552/ijam.v9i4.1110 ↗
19. Determination of Fluoride Content in Teas and Herbal Products Popular in Poland10.1007/s40201-022-00811-4 ↗
20. Fluoride Levels in Various Black Tea, Herbal and Fruit Infusions Consumed in Turkey10.1016/j.fct.2009.03.036 ↗
21. Comparison of Fluoride Levels (Total and Extracted) in Young, Old Tea Leaves and Market Tea Samples along with Impact of Tea Infusion on Dental Fluorosis in Fluoride Endemic Villages of Nalgonda District, India
22. Fluoride in Drinking Water, Brick Tea Infusion and Human Urine in Two Counties in Inner Mongolia, China10.1016/j.jhazmat.2009.01.094 ↗
23. Fluoride Contents in Tea and Soil from Tea Plantations and the Release of Fluoride into Tea Liquor during Infusion10.1016/S0269-7491(98)00187-0 ↗
24. Critical Factors Determining Fluoride Concentration in Tea Leaves Produced from Anhui Province, China10.1016/j.ecoenv.2016.04.023 ↗
25. Critical Review of Fluoride in Tea Plants (Camellia sinensis): Absorption, Transportation, Tolerance Mechanisms, and Defluorination Measures10.48130/bpr-0024-0010 ↗
26. Monitoring Fluorine Levels in Tea Leaves from Major Producing Areas in China and the Relative Health Risk10.1016/j.jfca.2023.105205 ↗
27. Fluoride Levels in Various Black Tea Commodities: Measurement and Safety Evaluation10.1016/j.fct.2006.01.010 ↗
28. Mazurek, A., Kowalska, G., Włodarczyk-Stasiak, M., Wyrostek, J., and Kowalski, R. (2023). The Influence of the Preparation of Tea Infusion on the Content of Fluoride and the Assessment of Health Risk for the Consumer in Poland. Appl. Sci., 13.10.3390/app13085075 ↗
29. Processing Procedures of Brick Tea and Their Influence on Fluorine Content10.1016/S0278-6915(01)00039-4 ↗
30. Potentiometric Analysis of Fluoride in Commonly Consumed Beverages: Method Development, Evaluation, and Risk Assessment10.1016/j.jfca.2024.106836 ↗
31. Determination of Fluoride Content in Tea Infusion by Using Fluoride Ion-Selective Electrode10.1016/S1452-3981(23)13924-1 ↗
32. Effect of Brewing Time and Water Hardness on Fluoride Release from Different Iranian Teas
33. Fluoride Content in Tea and Its Relationship with Tea Quality10.1021/jf0308354 ↗
34. Does the Addition of Spices Change the Content of Fluoride and Antioxidants in Black Tea Infusions?
35. Evaluation of PICO as a Knowledge Representation for Clinical Questions
36. Homa, K., Zakrzewski, W., Dobrzyński, W., Piszko, P.J., Piszko, A., Matys, J., Wiglusz, R.J., and Dobrzyński, M. (2024). Surface Functionalization of Titanium-Based Implants with a Nanohydroxyapatite Layer and Its Impact on Osteoblasts: A Systematic Review. J. Funct. Biomater., 15.10.3390/jfb15020045 ↗
37. Kensy, J., Dobrzyński, M., Wiench, R., Grzech-Leśniak, K., and Matys, J. (2021). Fibroblasts Adhesion to Laser-Modified Titanium Surfaces—A Systematic Review. Materials, 14.10.3390/ma14237305 ↗
38. Kiryk, J., Kiryk, S., Kensy, J., Świenc, W., Palka, B., Zimoląg-Dydak, M., Dobrzyński, W., Matys, J., and Dobrzyński, M. (2024). Effectiveness of Laser-Assisted Teeth Bleaching: A Systematic Review. Appl. Sci., 14.10.3390/app14209219 ↗
39. Matys, J., Kensy, J., Gedrange, T., Zawiślak, I., Grzech-Leśniak, K., and Dobrzyński, M. (2024). A Molecular Approach for Detecting Bacteria and Fungi in Healthcare Environment Aerosols: A Systematic Review. Int. J. Mol. Sci., 25.10.3390/ijms25084154 ↗
40. Piszko, P.J., Piszko, A., Kiryk, J., Lubojański, A., Dobrzyński, W., Wiglusz, R.J., Matys, J., and Dobrzyński, M. (2024). The Influence of Fluoride Gels on the Physicochemical Properties of Tooth Tissues and Dental Materials—A Systematic Review. Gels, 10.10.3390/gels10020098 ↗
41. Oleniacz-Trawińska, M., Kotela, A., Kensy, J., Kiryk, S., Dobrzyński, W., Kiryk, J., Gerber, H., Fast, M., Matys, J., and Dobrzyński, M. (2025). Evaluation of Factors Affecting Fluoride Release from Compomer Restorative Materials: A Systematic Review. Materials, 18.10.3390/ma18071627 ↗
42. Tokarczuk, D., Tokarczuk, O., Kiryk, J., Kensy, J., Szablińska, M., Dyl, T., Dobrzyński, W., Matys, J., and Dobrzyński, M. (2024). Fluoride Release by Restorative Materials after the Application of Surface Coating Agents: A Systematic Review. Appl. Sci., 14.10.3390/app14114956 ↗
43. Morawska-Wilk, A., Kensy, J., Kiryk, S., Kotela, A., Kiryk, J., Michalak, M., Grychowska, N., Fast, M., Matys, J., and Dobrzyński, M. (2025). Evaluation of Factors Influencing Fluoride Release from Dental Nanocomposite Materials: A Systematic Review. Nanomaterials, 15.10.3390/nano15090651 ↗
44. Struzik, N., Kensy, J., Piszko, P.J., Kiryk, J., Wiśniewska, K., Kiryk, S., Korjat, Ł., Horodniczy, T., Sobierajska, P., and Matys, J. (2024). Contamination in Bone Substitute Materials: A Systematic Review. Appl. Sci., 14.10.3390/app14188266 ↗
45. Method Agreement Analysis: A Review of Correct Methodology10.1016/j.theriogenology.2010.01.003 ↗
46. (2020). Chapter 3: Systematic Reviews of Effectiveness. JBI Manual for Evidence Synthesis, JBI.
47. Single Quadrupole Inductively Coupled Plasma-Mass Spectrometry for the Measurement of Fluorine in Tea Infusions and Its Health Risk Assessment10.1016/j.jfca.2019.103378 ↗
48. da S.; Carasek, E.; Welz, B. Determination of Fluorine in Tea Using High-Resolution Molecular Absorption Spectrometry with Electrothermal Vaporization of the Calcium Mono-Fluoride CaF10.1016/j.talanta.2011.08.044 ↗
49. Determination of Fluoride in Black, Green and Herbal Teas by Ionselective Electrode Using a Standard-Addition Method10.20473/j.djmkg.v38.i2.p91-95 ↗
50. Estimation of Fluoride Concentration in Tea Infusions, Prepared from Different Forms of Tea, Commercially Available in Mathura City10.4103/2231-0762.109371 ↗
51. Potentiometric Determination of Fluoride Release from Three Types of Tea Leaves10.1016/S1452-3981(23)13175-0 ↗
52. Human Exposure Assessment of Fluoride from Tea (Camellia Sinensis L.): A UK Based Issue?10.1016/j.foodres.2013.01.025 ↗
53. Fluoride Content of Soft Drinks, Nectars, Juices, Juice Drinks, Concentrates, Teas and Infusions Marketed in Portugal10.1080/19440049.2013.785636 ↗
54. Levels of Fluoride in the Ethiopian and Imported Black Tea (Camellia sinensis) Infusions Prepared in Tap and Fluoride-Rich Natural Waters10.1515/ijfe-2014-0133 ↗
55. Fluoride Concentrations in Traditional and Herbal Teas: Health Risk Assessment
56. Safety Evaluation of Fluoride Content in Tea Infusions Consumed in the Azores—A Volcanic Region with Water Springs Naturally Enriched in Fluoride10.1007/s12011-017-0947-9 ↗
57. A New Method for Determination of Fluoride Ion in Commodity Tea by Ion-Exclusion Chromatography10.1080/19476337.2018.1441188 ↗
58. Risk Assessment of Fluoride Daily Intake from Preference Beverage10.1016/j.jds.2020.05.023 ↗
59. The Use of Microwave-Induced Plasma Optical Emission Spectrometry for Fluorine Determination and Its Application to Tea Infusions10.1016/j.talanta.2021.122190 ↗
60. Analysis of the Fluoride Levels of Well Water and Tea Consumed by the Moroccan Population in Different Rural Areas10.1016/j.matpr.2022.07.360 ↗
61. The Effects of Color and Brightness of Brewed Black Tea on Its Fluoride Concentration in Türkiye10.33483/jfpau.1298235 ↗
62. Fluoride Content of Iranian Black Tea and Tea Liquor
63. Exposure Assessment of Fluoride Intake Through Commercially Available Black Tea (Camellia sinensis L.) from Areas with High Incidences of Chronic Kidney Disease with Undetermined Origin (CKDu) in Sri Lanka10.1007/s12011-021-02694-2 ↗
64. Intake of Fluoride and Other Hofmeister Ions from Black Tea Consumption in CKDu Prevalent Areas, Sri Lanka10.1007/s11356-022-25076-0 ↗
65. Fluoride Content in Various Types of Tea Used by Tea Stalls in Salem District—An in Vitro Cross Sectional Study10.4103/jomfp.jomfp_346_22 ↗
66. Contents of Fluoride, Lead, Copper, Chromium, Arsenic and Cadmium in Chinese Pu-Erh Tea10.1016/j.foodres.2012.06.014 ↗
67. Determination of Total and Fluoride Bound Aluminium in Tea Infusions by Ion Selective Electrode and Flame Atomic Absorption Spectrometry10.1080/00032710008543140 ↗
68. The Characteristics of Dissolution of Fluoride and Aluminum from Brick Tea10.1007/BF02873802 ↗
69. Assessment and Health Risk of Fluoride from Northeast Indian Tea (Camellia sinensis L.): Fixing up the Maximum Residue Level of Fluoride in Tea10.1016/j.jfca.2023.105928 ↗
70. Prevalence of Brick Tea-Type Fluorosis in the Tibet Autonomous Region10.2188/jea.JE20150037 ↗
71. Skeletal fluorosis related to habitual tea consumption: Long-term follow-up after reduction and discontinuation of tea10.4158/EP171886.CR ↗
72. Johnson, J.E.H., Kearns, A.E., Doran, P.M., Khoo, T.K., and Wermers, R.A. (2007). Fluoride-related bone disease associated with habitual tea consumption. Mayo Clinic Proceedings, Elsevier.10.1016/S0025-6196(11)61192-0 ↗
73. Skeletal fluorosis from instant tea10.1359/jbmr.080101 ↗
74. Skeletal fluorosis from brewed tea10.1210/jc.2010-2891 ↗